Fully Automated Microsystem for Unmediated Electrochemical Characterization, Visualization and Monitoring of Bacteria on Solid Media; <i>E. coli</i> K-12: A Case Study

• Main Authors: Cesar A. Hernandez, Valerio Beni, Johann F. Osma
• Format: Article
• Language: English
• Published: MDPI AG 2019-11-01
• Series: Biosensors
• Subjects: bio-electrochemical systems; microsystem; bacteria; direct electron transfer; cyclic voltammetry; electrochemical impedance spectrometry; microscopy; automated system
• Online Access: https://www.mdpi.com/2079-6374/9/4/131

In this paper, we present a non-fluidic microsystem for the simultaneous visualization and electrochemical evaluation of confined, growing bacteria on solid media. Using a completely automated platform, real-time monitoring of bacterial and image-based computer characterization of growth were performed. Electrochemical tests, using <i>Escherichia coli</i> K-12 as the model microorganism, revealed the development of a faradaic process at the bacteria&#8722;microelectrode interface inside the microsystem, as implied by cyclic voltammetry and electrochemical impedance spectrometry measurements. The electrochemical information was used to determine the moment in which bacteria colonized the electrode-enabled area of the microsystem. This microsystem shows potential advantages for long-term electrochemical monitoring of the extracellular environment of cell culture and has been designed using readily available technologies that can be easily integrated in routine protocols. Complementarily, these methods can help elucidate fundamental questions of the electron transfer of bacterial cultures and are potentially feasible to be integrated into current characterization techniques.