Screening and Identification of Differentially Expressed Genes Expressed among Left and Right Colon Adenocarcinoma
Purpose. Colon adenocarcinoma (COAD) is the third most common malignancy globally and is further categorized as left colon adenocarcinoma (LCOAD) or right colon adenocarcinoma (RCOAD) depending on the location of the primary tumor. The therapeutic outcome and long-term prognosis for patients with CO...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2020-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2020/8465068 |
id |
doaj-dd25b3d6da28416eb2cab845a218a15e |
---|---|
record_format |
Article |
spelling |
doaj-dd25b3d6da28416eb2cab845a218a15e2020-11-24T21:39:51ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/84650688465068Screening and Identification of Differentially Expressed Genes Expressed among Left and Right Colon AdenocarcinomaJing Han0Xue Zhang1Yang Yang2Li Feng3Gui-Ying Wang4Nan Zhang5Department of Medical Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, ChinaDepartment of Medical Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, ChinaSecond Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, ChinaDepartment of Medical Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, ChinaSecond Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, ChinaPurpose. Colon adenocarcinoma (COAD) is the third most common malignancy globally and is further categorized as left colon adenocarcinoma (LCOAD) or right colon adenocarcinoma (RCOAD) depending on the location of the primary tumor. The therapeutic outcome and long-term prognosis for patients with COAD are less than satisfactory, and this may be associated with tumor location. Therefore, it is important to investigate the genetic differences in COAD at different sites. Patients and Methods. Public data associated with COAD were downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified using R software (version 3.5.3), and functional annotation of DEGs was performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. A protein-protein interaction network was constructed, hub genes were identified and analyzed, and data mining using Gene Expression Profiling Interactive Analysis (GEPIA) was conducted. Results. A total of 286 DEGs were identified between LCOAD and RCOAD. Additionally, 10 hub genes associated with COAD at different locations were screened, namely, CDKN2A, IGF1R, MDM2, SMAD3, SLC2A1, GRM5, PLCB4, FGFR1, UBE2V2, and TNFRSF10B. The expression of cyclin-dependent kinase inhibitor 2A (CDKN2A) and solute carrier family 2 member 1 (SLC2A1) was significantly associated with pathological stage P<0.05. COAD patients with high expression levels of CDKN2A exhibited poorer overall survival (OS) times than those with low expression levels P<0.05. Conclusion. CDKN2A expression was significantly different between LCOAD and RCOAD and was closely related to the prognosis of COAD. It is of great value for further understanding of the pathogenesis of LCOAD and RCOAD.http://dx.doi.org/10.1155/2020/8465068 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jing Han Xue Zhang Yang Yang Li Feng Gui-Ying Wang Nan Zhang |
spellingShingle |
Jing Han Xue Zhang Yang Yang Li Feng Gui-Ying Wang Nan Zhang Screening and Identification of Differentially Expressed Genes Expressed among Left and Right Colon Adenocarcinoma BioMed Research International |
author_facet |
Jing Han Xue Zhang Yang Yang Li Feng Gui-Ying Wang Nan Zhang |
author_sort |
Jing Han |
title |
Screening and Identification of Differentially Expressed Genes Expressed among Left and Right Colon Adenocarcinoma |
title_short |
Screening and Identification of Differentially Expressed Genes Expressed among Left and Right Colon Adenocarcinoma |
title_full |
Screening and Identification of Differentially Expressed Genes Expressed among Left and Right Colon Adenocarcinoma |
title_fullStr |
Screening and Identification of Differentially Expressed Genes Expressed among Left and Right Colon Adenocarcinoma |
title_full_unstemmed |
Screening and Identification of Differentially Expressed Genes Expressed among Left and Right Colon Adenocarcinoma |
title_sort |
screening and identification of differentially expressed genes expressed among left and right colon adenocarcinoma |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
Purpose. Colon adenocarcinoma (COAD) is the third most common malignancy globally and is further categorized as left colon adenocarcinoma (LCOAD) or right colon adenocarcinoma (RCOAD) depending on the location of the primary tumor. The therapeutic outcome and long-term prognosis for patients with COAD are less than satisfactory, and this may be associated with tumor location. Therefore, it is important to investigate the genetic differences in COAD at different sites. Patients and Methods. Public data associated with COAD were downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified using R software (version 3.5.3), and functional annotation of DEGs was performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. A protein-protein interaction network was constructed, hub genes were identified and analyzed, and data mining using Gene Expression Profiling Interactive Analysis (GEPIA) was conducted. Results. A total of 286 DEGs were identified between LCOAD and RCOAD. Additionally, 10 hub genes associated with COAD at different locations were screened, namely, CDKN2A, IGF1R, MDM2, SMAD3, SLC2A1, GRM5, PLCB4, FGFR1, UBE2V2, and TNFRSF10B. The expression of cyclin-dependent kinase inhibitor 2A (CDKN2A) and solute carrier family 2 member 1 (SLC2A1) was significantly associated with pathological stage P<0.05. COAD patients with high expression levels of CDKN2A exhibited poorer overall survival (OS) times than those with low expression levels P<0.05. Conclusion. CDKN2A expression was significantly different between LCOAD and RCOAD and was closely related to the prognosis of COAD. It is of great value for further understanding of the pathogenesis of LCOAD and RCOAD. |
url |
http://dx.doi.org/10.1155/2020/8465068 |
work_keys_str_mv |
AT jinghan screeningandidentificationofdifferentiallyexpressedgenesexpressedamongleftandrightcolonadenocarcinoma AT xuezhang screeningandidentificationofdifferentiallyexpressedgenesexpressedamongleftandrightcolonadenocarcinoma AT yangyang screeningandidentificationofdifferentiallyexpressedgenesexpressedamongleftandrightcolonadenocarcinoma AT lifeng screeningandidentificationofdifferentiallyexpressedgenesexpressedamongleftandrightcolonadenocarcinoma AT guiyingwang screeningandidentificationofdifferentiallyexpressedgenesexpressedamongleftandrightcolonadenocarcinoma AT nanzhang screeningandidentificationofdifferentiallyexpressedgenesexpressedamongleftandrightcolonadenocarcinoma |
_version_ |
1716682016581222400 |