Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3

Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3

The terminal portion of the pol gene and the entire env gene of the human endogenous retrovirus ERV3 was screened for polymorphic nucleotides. For this purpose fragments amplified from the desired regions of ERV3 were subjected to single strand conformational analysis (SSCP analysis). Using this app...

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Main Authors: H. B. Rasmussen, J. Clausen
Format: Article
Language:English
Published: Hindawi Limited 1998-01-01
Series:Disease Markers
Online Access:http://dx.doi.org/10.1155/1998/958379
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spelling doaj-dd3b2212abb24c139a7136f8336114b52020-11-24T22:18:49ZengHindawi LimitedDisease Markers0278-02401875-86301998-01-0114312713310.1155/1998/958379Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3H. B. Rasmussen0J. Clausen1Department of Life Science and Chemistry, Roskilde University, PO Box 260, DK-4000 Roskilde, DenmarkDepartment of Life Science and Chemistry, Roskilde University, PO Box 260, DK-4000 Roskilde, DenmarkThe terminal portion of the pol gene and the entire env gene of the human endogenous retrovirus ERV3 was screened for polymorphic nucleotides. For this purpose fragments amplified from the desired regions of ERV3 were subjected to single strand conformational analysis (SSCP analysis). Using this approach, we detected 13 polymorphic nucleotides, namely four in the pol gene and nine in the env gene. Three of the nucleotide substitutions were synonymous (not affecting the amino acid code). One of the non-synonymous nucleotide substitutions changed an arginine codon to a termination codon. The alleles at the different polymorphic sites could be arranged into five ERV3 haplotypes, two of which were new.http://dx.doi.org/10.1155/1998/958379
collection DOAJ
language English
format Article
sources DOAJ
author H. B. Rasmussen
J. Clausen
spellingShingle H. B. Rasmussen
J. Clausen
Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3
Disease Markers
author_facet H. B. Rasmussen
J. Clausen
author_sort H. B. Rasmussen
title Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3
title_short Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3
title_full Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3
title_fullStr Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3
title_full_unstemmed Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3
title_sort large number of polymorphic nucleotides and a termination codon in the env gene of the endogenous human retrovirus erv3
publisher Hindawi Limited
series Disease Markers
issn 0278-0240
1875-8630
publishDate 1998-01-01
description The terminal portion of the pol gene and the entire env gene of the human endogenous retrovirus ERV3 was screened for polymorphic nucleotides. For this purpose fragments amplified from the desired regions of ERV3 were subjected to single strand conformational analysis (SSCP analysis). Using this approach, we detected 13 polymorphic nucleotides, namely four in the pol gene and nine in the env gene. Three of the nucleotide substitutions were synonymous (not affecting the amino acid code). One of the non-synonymous nucleotide substitutions changed an arginine codon to a termination codon. The alleles at the different polymorphic sites could be arranged into five ERV3 haplotypes, two of which were new.
url http://dx.doi.org/10.1155/1998/958379
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AT jclausen largenumberofpolymorphicnucleotidesandaterminationcodonintheenvgeneoftheendogenoushumanretroviruserv3
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