Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3
The terminal portion of the pol gene and the entire env gene of the human endogenous retrovirus ERV3 was screened for polymorphic nucleotides. For this purpose fragments amplified from the desired regions of ERV3 were subjected to single strand conformational analysis (SSCP analysis). Using this app...
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1998-01-01
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Series: | Disease Markers |
Online Access: | http://dx.doi.org/10.1155/1998/958379 |
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doaj-dd3b2212abb24c139a7136f8336114b52020-11-24T22:18:49ZengHindawi LimitedDisease Markers0278-02401875-86301998-01-0114312713310.1155/1998/958379Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3H. B. Rasmussen0J. Clausen1Department of Life Science and Chemistry, Roskilde University, PO Box 260, DK-4000 Roskilde, DenmarkDepartment of Life Science and Chemistry, Roskilde University, PO Box 260, DK-4000 Roskilde, DenmarkThe terminal portion of the pol gene and the entire env gene of the human endogenous retrovirus ERV3 was screened for polymorphic nucleotides. For this purpose fragments amplified from the desired regions of ERV3 were subjected to single strand conformational analysis (SSCP analysis). Using this approach, we detected 13 polymorphic nucleotides, namely four in the pol gene and nine in the env gene. Three of the nucleotide substitutions were synonymous (not affecting the amino acid code). One of the non-synonymous nucleotide substitutions changed an arginine codon to a termination codon. The alleles at the different polymorphic sites could be arranged into five ERV3 haplotypes, two of which were new.http://dx.doi.org/10.1155/1998/958379 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
H. B. Rasmussen J. Clausen |
spellingShingle |
H. B. Rasmussen J. Clausen Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3 Disease Markers |
author_facet |
H. B. Rasmussen J. Clausen |
author_sort |
H. B. Rasmussen |
title |
Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3 |
title_short |
Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3 |
title_full |
Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3 |
title_fullStr |
Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3 |
title_full_unstemmed |
Large Number of Polymorphic Nucleotides and a Termination Codon in the env Gene of the Endogenous Human Retrovirus ERV3 |
title_sort |
large number of polymorphic nucleotides and a termination codon in the env gene of the endogenous human retrovirus erv3 |
publisher |
Hindawi Limited |
series |
Disease Markers |
issn |
0278-0240 1875-8630 |
publishDate |
1998-01-01 |
description |
The terminal portion of the pol gene and the entire env gene of the human endogenous retrovirus ERV3 was screened for polymorphic nucleotides. For this purpose fragments amplified from the desired regions of ERV3 were subjected to single strand conformational analysis (SSCP analysis). Using this approach, we detected 13 polymorphic nucleotides, namely four in the pol gene and nine in the env gene. Three of the nucleotide substitutions were synonymous (not affecting the amino acid code). One of the non-synonymous nucleotide substitutions changed an arginine codon to a termination codon. The alleles at the different polymorphic sites could be arranged into five ERV3 haplotypes, two of which were new. |
url |
http://dx.doi.org/10.1155/1998/958379 |
work_keys_str_mv |
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_version_ |
1725781453397032960 |