The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i>

The <i>velvet</i> regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus <i>Aspergillus nidulans</i>. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in <i>A</i>. <i>nidulans</i>...

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Main Authors: Min-Ju Kim, Mi-Kyung Lee, Huy Quang Pham, Myeong Ju Gu, Bohan Zhu, Sung-Hun Son, Dongyup Hahn, Jae-Ho Shin, Jae-Hyuk Yu, Hee-Soo Park, Kap-Hoon Han
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Genes
Subjects:
Online Access:https://www.mdpi.com/2073-4425/11/1/103
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spelling doaj-dd806e9f189a4fae8368d60e07ea37072020-11-25T02:06:05ZengMDPI AGGenes2073-44252020-01-0111110310.3390/genes11010103genes11010103The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i>Min-Ju Kim0Mi-Kyung Lee1Huy Quang Pham2Myeong Ju Gu3Bohan Zhu4Sung-Hun Son5Dongyup Hahn6Jae-Ho Shin7Jae-Hyuk Yu8Hee-Soo Park9Kap-Hoon Han10School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaBiological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 56212, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaDepartment of Pharmaceutical Engineering, Woosuk University, Wanju 55338, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaDepartments of Bacteriology and Genetics, University of Wisconsin, Madison, WI 53706, USASchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaDepartment of Pharmaceutical Engineering, Woosuk University, Wanju 55338, KoreaThe <i>velvet</i> regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus <i>Aspergillus nidulans</i>. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in <i>A</i>. <i>nidulans</i>. During ascospore maturation, the deletion of <i>vosA</i> causes a rapid decrease in spore viability. The absence of <i>vosA</i> also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of <i>vosA</i> leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of <i>vosA</i> causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of <i>vosA</i> results in the downregulation of various genes involved in primary metabolism. In addition, <i>vosA</i> deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the <i>velvet</i> regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in <i>A. nidulans</i>.https://www.mdpi.com/2073-4425/11/1/103vosasexual developmentascospores<i>aspergillus nidulans</i>
collection DOAJ
language English
format Article
sources DOAJ
author Min-Ju Kim
Mi-Kyung Lee
Huy Quang Pham
Myeong Ju Gu
Bohan Zhu
Sung-Hun Son
Dongyup Hahn
Jae-Ho Shin
Jae-Hyuk Yu
Hee-Soo Park
Kap-Hoon Han
spellingShingle Min-Ju Kim
Mi-Kyung Lee
Huy Quang Pham
Myeong Ju Gu
Bohan Zhu
Sung-Hun Son
Dongyup Hahn
Jae-Ho Shin
Jae-Hyuk Yu
Hee-Soo Park
Kap-Hoon Han
The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i>
Genes
vosa
sexual development
ascospores
<i>aspergillus nidulans</i>
author_facet Min-Ju Kim
Mi-Kyung Lee
Huy Quang Pham
Myeong Ju Gu
Bohan Zhu
Sung-Hun Son
Dongyup Hahn
Jae-Ho Shin
Jae-Hyuk Yu
Hee-Soo Park
Kap-Hoon Han
author_sort Min-Ju Kim
title The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i>
title_short The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i>
title_full The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i>
title_fullStr The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i>
title_full_unstemmed The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i>
title_sort <i>velvet</i> regulator vosa governs survival and secondary metabolism of sexual spores in <i>aspergillus nidulans</i>
publisher MDPI AG
series Genes
issn 2073-4425
publishDate 2020-01-01
description The <i>velvet</i> regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus <i>Aspergillus nidulans</i>. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in <i>A</i>. <i>nidulans</i>. During ascospore maturation, the deletion of <i>vosA</i> causes a rapid decrease in spore viability. The absence of <i>vosA</i> also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of <i>vosA</i> leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of <i>vosA</i> causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of <i>vosA</i> results in the downregulation of various genes involved in primary metabolism. In addition, <i>vosA</i> deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the <i>velvet</i> regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in <i>A. nidulans</i>.
topic vosa
sexual development
ascospores
<i>aspergillus nidulans</i>
url https://www.mdpi.com/2073-4425/11/1/103
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