The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i>
The <i>velvet</i> regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus <i>Aspergillus nidulans</i>. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in <i>A</i>. <i>nidulans</i>...
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doaj-dd806e9f189a4fae8368d60e07ea37072020-11-25T02:06:05ZengMDPI AGGenes2073-44252020-01-0111110310.3390/genes11010103genes11010103The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i>Min-Ju Kim0Mi-Kyung Lee1Huy Quang Pham2Myeong Ju Gu3Bohan Zhu4Sung-Hun Son5Dongyup Hahn6Jae-Ho Shin7Jae-Hyuk Yu8Hee-Soo Park9Kap-Hoon Han10School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaBiological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 56212, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaDepartment of Pharmaceutical Engineering, Woosuk University, Wanju 55338, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaDepartments of Bacteriology and Genetics, University of Wisconsin, Madison, WI 53706, USASchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaDepartment of Pharmaceutical Engineering, Woosuk University, Wanju 55338, KoreaThe <i>velvet</i> regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus <i>Aspergillus nidulans</i>. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in <i>A</i>. <i>nidulans</i>. During ascospore maturation, the deletion of <i>vosA</i> causes a rapid decrease in spore viability. The absence of <i>vosA</i> also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of <i>vosA</i> leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of <i>vosA</i> causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of <i>vosA</i> results in the downregulation of various genes involved in primary metabolism. In addition, <i>vosA</i> deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the <i>velvet</i> regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in <i>A. nidulans</i>.https://www.mdpi.com/2073-4425/11/1/103vosasexual developmentascospores<i>aspergillus nidulans</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Min-Ju Kim Mi-Kyung Lee Huy Quang Pham Myeong Ju Gu Bohan Zhu Sung-Hun Son Dongyup Hahn Jae-Ho Shin Jae-Hyuk Yu Hee-Soo Park Kap-Hoon Han |
spellingShingle |
Min-Ju Kim Mi-Kyung Lee Huy Quang Pham Myeong Ju Gu Bohan Zhu Sung-Hun Son Dongyup Hahn Jae-Ho Shin Jae-Hyuk Yu Hee-Soo Park Kap-Hoon Han The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i> Genes vosa sexual development ascospores <i>aspergillus nidulans</i> |
author_facet |
Min-Ju Kim Mi-Kyung Lee Huy Quang Pham Myeong Ju Gu Bohan Zhu Sung-Hun Son Dongyup Hahn Jae-Ho Shin Jae-Hyuk Yu Hee-Soo Park Kap-Hoon Han |
author_sort |
Min-Ju Kim |
title |
The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i> |
title_short |
The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i> |
title_full |
The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i> |
title_fullStr |
The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i> |
title_full_unstemmed |
The <i>velvet</i> Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in <i>Aspergillus nidulans</i> |
title_sort |
<i>velvet</i> regulator vosa governs survival and secondary metabolism of sexual spores in <i>aspergillus nidulans</i> |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2020-01-01 |
description |
The <i>velvet</i> regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus <i>Aspergillus nidulans</i>. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in <i>A</i>. <i>nidulans</i>. During ascospore maturation, the deletion of <i>vosA</i> causes a rapid decrease in spore viability. The absence of <i>vosA</i> also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of <i>vosA</i> leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of <i>vosA</i> causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of <i>vosA</i> results in the downregulation of various genes involved in primary metabolism. In addition, <i>vosA</i> deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the <i>velvet</i> regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in <i>A. nidulans</i>. |
topic |
vosa sexual development ascospores <i>aspergillus nidulans</i> |
url |
https://www.mdpi.com/2073-4425/11/1/103 |
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