Characterization of microRNAs of Beta macrocarpa and their responses to Beet necrotic yellow vein virus infection.
Plant microRNAs (miRNAs) are a class of non-coding RNAs that play important roles in plant development, defense, and symptom development. Here, 547 known miRNAs representing 129 miRNA families, and 282 potential novel miRNAs were identified in Beta macrocarpa using small RNA deep sequencing. A phylo...
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doaj-dd9fd783045748859baaf225c6aab01d2020-11-25T01:14:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011210e018650010.1371/journal.pone.0186500Characterization of microRNAs of Beta macrocarpa and their responses to Beet necrotic yellow vein virus infection.Jun-Ying LiuHui-Yan FanYing WangYong-Liang ZhangDa-Wei LiJia-Lin YuCheng-Gui HanPlant microRNAs (miRNAs) are a class of non-coding RNAs that play important roles in plant development, defense, and symptom development. Here, 547 known miRNAs representing 129 miRNA families, and 282 potential novel miRNAs were identified in Beta macrocarpa using small RNA deep sequencing. A phylogenetic analysis was performed, and 8 Beta lineage-specific miRNAs were identified. Through a differential expression analysis, miRNAs associated with Beet necrotic yellow vein virus (BNYVV) infection were identified and confirmed using a microarray analysis and stem-loop RT-qPCR. In total, 103 known miRNAs representing 38 miRNA families, and 45 potential novel miRNAs were differentially regulated, with at least a two-fold change, in BNYVV-infected plants compared with that of the mock-inoculated control. Targets of these differentially expressed miRNAs were also predicted by degradome sequencing. These differentially expressed miRNAs were involved in hormone biosynthesis and signal transduction pathways, and enhanced axillary bud development and plant defenses. This work is the first to describe miRNAs of the plant genus Beta and may offer a reference for miRNA research in other species in the genus. It provides valuable information on the pathogenicity mechanisms of BNYVV.http://europepmc.org/articles/PMC5643120?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun-Ying Liu Hui-Yan Fan Ying Wang Yong-Liang Zhang Da-Wei Li Jia-Lin Yu Cheng-Gui Han |
spellingShingle |
Jun-Ying Liu Hui-Yan Fan Ying Wang Yong-Liang Zhang Da-Wei Li Jia-Lin Yu Cheng-Gui Han Characterization of microRNAs of Beta macrocarpa and their responses to Beet necrotic yellow vein virus infection. PLoS ONE |
author_facet |
Jun-Ying Liu Hui-Yan Fan Ying Wang Yong-Liang Zhang Da-Wei Li Jia-Lin Yu Cheng-Gui Han |
author_sort |
Jun-Ying Liu |
title |
Characterization of microRNAs of Beta macrocarpa and their responses to Beet necrotic yellow vein virus infection. |
title_short |
Characterization of microRNAs of Beta macrocarpa and their responses to Beet necrotic yellow vein virus infection. |
title_full |
Characterization of microRNAs of Beta macrocarpa and their responses to Beet necrotic yellow vein virus infection. |
title_fullStr |
Characterization of microRNAs of Beta macrocarpa and their responses to Beet necrotic yellow vein virus infection. |
title_full_unstemmed |
Characterization of microRNAs of Beta macrocarpa and their responses to Beet necrotic yellow vein virus infection. |
title_sort |
characterization of micrornas of beta macrocarpa and their responses to beet necrotic yellow vein virus infection. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Plant microRNAs (miRNAs) are a class of non-coding RNAs that play important roles in plant development, defense, and symptom development. Here, 547 known miRNAs representing 129 miRNA families, and 282 potential novel miRNAs were identified in Beta macrocarpa using small RNA deep sequencing. A phylogenetic analysis was performed, and 8 Beta lineage-specific miRNAs were identified. Through a differential expression analysis, miRNAs associated with Beet necrotic yellow vein virus (BNYVV) infection were identified and confirmed using a microarray analysis and stem-loop RT-qPCR. In total, 103 known miRNAs representing 38 miRNA families, and 45 potential novel miRNAs were differentially regulated, with at least a two-fold change, in BNYVV-infected plants compared with that of the mock-inoculated control. Targets of these differentially expressed miRNAs were also predicted by degradome sequencing. These differentially expressed miRNAs were involved in hormone biosynthesis and signal transduction pathways, and enhanced axillary bud development and plant defenses. This work is the first to describe miRNAs of the plant genus Beta and may offer a reference for miRNA research in other species in the genus. It provides valuable information on the pathogenicity mechanisms of BNYVV. |
url |
http://europepmc.org/articles/PMC5643120?pdf=render |
work_keys_str_mv |
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