Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen
Abstract CRISPR-based genome perturbation provides a new avenue to conveniently change DNA sequences, transcription, and epigenetic modifications in genetic screens. However, it remains challenging to assay the complex molecular readouts after perturbation at high resolution and at scale. By introdu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-06-01
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| Series: | Genome Biology |
| Online Access: | http://link.springer.com/article/10.1186/s13059-020-02044-w |