Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen

Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen

Abstract CRISPR-based genome perturbation provides a new avenue to conveniently change DNA sequences, transcription, and epigenetic modifications in genetic screens. However, it remains challenging to assay the complex molecular readouts after perturbation at high resolution and at scale. By introdu...

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Bibliographic Details
Main Authors:	Qingkai Song, Ke Ni, Min Liu, Yini Li, Lixia Wang, Yingying Wang, Yingzheng Liu, Zhenxing Yu, Yinyao Qi, Zhike Lu, Lijia Ma
Format:	Article
Language:	English
Published:	BMC 2020-06-01
Series:	Genome Biology
Online Access:	http://link.springer.com/article/10.1186/s13059-020-02044-w

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