Evaluation of Predation Capability of Periodontopathogens Bacteria by Bdellovibrio Bacteriovorus HD100. An in Vitro Study
Treatment options against periodontitis attempt to completely remove oral microbiota even if several species in dental plaque demonstrate protective features. Predatory bacteria that selectively predate solely on Gram-negative bacteria might be a viable therapeutic alternative. Therefore, the aim of...
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doaj-dda6a14bb4184dd08764fa15a11b5ebd2020-11-24T21:20:55ZengMDPI AGMaterials1996-19442019-06-011212200810.3390/ma12122008ma12122008Evaluation of Predation Capability of Periodontopathogens Bacteria by Bdellovibrio Bacteriovorus HD100. An in Vitro StudyRomeo Patini0Paola Cattani1Simona Marchetti2Gaetano Isola3Gianluca Quaranta4Patrizia Gallenzi5Fondazione Policlinico Universitario A. Gemelli IRCCS, Institute of Dentistry and Maxillofacial Surgery, Università Cattolica del Sacro Cuore, 00168 Roma, ItalyFondazione Policlinico Universitario A. Gemelli IRCCS, Institute of Microbiology and Virology, Università Cattolica del Sacro Cuore, 00168 Roma, ItalyFondazione Policlinico Universitario A. Gemelli IRCCS, Institute of Microbiology and Virology, Università Cattolica del Sacro Cuore, 00168 Roma, ItalyDepartment of General Surgery and Surgical-Medical Specialties, Via Plebiscito 628, University of Catania, 95124 Catania, ItalyFondazione Policlinico Universitario A. Gemelli IRCCS, Institute of Microbiology and Virology, Università Cattolica del Sacro Cuore, 00168 Roma, ItalyFondazione Policlinico Universitario A. Gemelli IRCCS, Institute of Dentistry and Maxillofacial Surgery, Università Cattolica del Sacro Cuore, 00168 Roma, ItalyTreatment options against periodontitis attempt to completely remove oral microbiota even if several species in dental plaque demonstrate protective features. Predatory bacteria that selectively predate solely on Gram-negative bacteria might be a viable therapeutic alternative. Therefore, the aim of this study is to in vitro evaluate the susceptibility of some oral pathogens to predation by <i>B. bacteriovorus</i> HD100 in liquid suspension. Cultures of prey cell were prepared in brain heart infusion broth (BHI) broth incubating overnight at the appropriate conditions for each organism to reach log phase of growth. Predatory activity was assessed by measuring optical density at 600 nm after 12, 24, 48 and 72 hours. Statistical analysis was performed using the Mann−Whitney U test and p values less than 0.05 were considered statistically significant. The study demonstrated that <i>B. bacteriovorus</i> is able to predate on aerobic species and on microaerophilic ones (p < 0.05) but also that its predatory capacity is strongly compromised by the conditions of anaerobiosis. <i>B. bacteriovorus</i>, in fact, was unable to predate the anaerobic species involved in the present study (<i>F. nucleatum</i> and <i>P. gingivalis</i>). The findings of the study suggest that <i>B. bacteriovorus</i> is able to tolerate microaerophilic conditions and that in anaerobiosis it cannot exert its predatory capacity. Such evidence could lead to its use as an agent to prevent recolonization of the periodontal pocket following therapy. Further studies are needed to investigate the activity of <i>B. bacteriovorus</i> against recently recognized periodontopathogens, alone or organized in biofilms of multi-species communities.https://www.mdpi.com/1996-1944/12/12/2008Local antimicrobial therapymicrobiologyperiodontal medicineperiodontitisplaque control |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Romeo Patini Paola Cattani Simona Marchetti Gaetano Isola Gianluca Quaranta Patrizia Gallenzi |
spellingShingle |
Romeo Patini Paola Cattani Simona Marchetti Gaetano Isola Gianluca Quaranta Patrizia Gallenzi Evaluation of Predation Capability of Periodontopathogens Bacteria by Bdellovibrio Bacteriovorus HD100. An in Vitro Study Materials Local antimicrobial therapy microbiology periodontal medicine periodontitis plaque control |
author_facet |
Romeo Patini Paola Cattani Simona Marchetti Gaetano Isola Gianluca Quaranta Patrizia Gallenzi |
author_sort |
Romeo Patini |
title |
Evaluation of Predation Capability of Periodontopathogens Bacteria by Bdellovibrio Bacteriovorus HD100. An in Vitro Study |
title_short |
Evaluation of Predation Capability of Periodontopathogens Bacteria by Bdellovibrio Bacteriovorus HD100. An in Vitro Study |
title_full |
Evaluation of Predation Capability of Periodontopathogens Bacteria by Bdellovibrio Bacteriovorus HD100. An in Vitro Study |
title_fullStr |
Evaluation of Predation Capability of Periodontopathogens Bacteria by Bdellovibrio Bacteriovorus HD100. An in Vitro Study |
title_full_unstemmed |
Evaluation of Predation Capability of Periodontopathogens Bacteria by Bdellovibrio Bacteriovorus HD100. An in Vitro Study |
title_sort |
evaluation of predation capability of periodontopathogens bacteria by bdellovibrio bacteriovorus hd100. an in vitro study |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2019-06-01 |
description |
Treatment options against periodontitis attempt to completely remove oral microbiota even if several species in dental plaque demonstrate protective features. Predatory bacteria that selectively predate solely on Gram-negative bacteria might be a viable therapeutic alternative. Therefore, the aim of this study is to in vitro evaluate the susceptibility of some oral pathogens to predation by <i>B. bacteriovorus</i> HD100 in liquid suspension. Cultures of prey cell were prepared in brain heart infusion broth (BHI) broth incubating overnight at the appropriate conditions for each organism to reach log phase of growth. Predatory activity was assessed by measuring optical density at 600 nm after 12, 24, 48 and 72 hours. Statistical analysis was performed using the Mann−Whitney U test and p values less than 0.05 were considered statistically significant. The study demonstrated that <i>B. bacteriovorus</i> is able to predate on aerobic species and on microaerophilic ones (p < 0.05) but also that its predatory capacity is strongly compromised by the conditions of anaerobiosis. <i>B. bacteriovorus</i>, in fact, was unable to predate the anaerobic species involved in the present study (<i>F. nucleatum</i> and <i>P. gingivalis</i>). The findings of the study suggest that <i>B. bacteriovorus</i> is able to tolerate microaerophilic conditions and that in anaerobiosis it cannot exert its predatory capacity. Such evidence could lead to its use as an agent to prevent recolonization of the periodontal pocket following therapy. Further studies are needed to investigate the activity of <i>B. bacteriovorus</i> against recently recognized periodontopathogens, alone or organized in biofilms of multi-species communities. |
topic |
Local antimicrobial therapy microbiology periodontal medicine periodontitis plaque control |
url |
https://www.mdpi.com/1996-1944/12/12/2008 |
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1726002219920130048 |