Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium

Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains pr...

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Main Authors: Yalda Basim, Ghasemali Mohebali, Sahand Jorfi, Ramin Nabizadeh, Mehdi Ahmadi Moghadam, Ata Ghadiri, Nematollah Jaafarzadeh Haghighi Fard
Format: Article
Language:English
Published: Kerman University of Medical Sciences 2020-06-01
Series:Environmental Health Engineering and Management
Subjects:
Online Access:http://ehemj.com/article-1-615-en.html
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spelling doaj-dda8f8de1d3345afa66a38ef865674b52020-11-25T03:41:06ZengKerman University of Medical SciencesEnvironmental Health Engineering and Management2423-37652423-43112020-06-017212713310.34172/EHEM.2020.15Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortiumYalda Basim0Ghasemali Mohebali1Sahand Jorfi2Ramin Nabizadeh3Mehdi Ahmadi Moghadam4Ata Ghadiri5Nematollah Jaafarzadeh Haghighi Fard6Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranMicrobiology and Biotechnology Research Group, Research Institute of Petroleum Industry, Tehran, IranEnvironmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, IranEnvironmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranEnvironmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID). Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research. Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained.http://ehemj.com/article-1-615-en.htmloil-contaminated soilsbiodegradationbacterial diversitytotal petroleum hydrocarbonsindigenous bacterial consortium
collection DOAJ
language English
format Article
sources DOAJ
author Yalda Basim
Ghasemali Mohebali
Sahand Jorfi
Ramin Nabizadeh
Mehdi Ahmadi Moghadam
Ata Ghadiri
Nematollah Jaafarzadeh Haghighi Fard
spellingShingle Yalda Basim
Ghasemali Mohebali
Sahand Jorfi
Ramin Nabizadeh
Mehdi Ahmadi Moghadam
Ata Ghadiri
Nematollah Jaafarzadeh Haghighi Fard
Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
Environmental Health Engineering and Management
oil-contaminated soils
biodegradation
bacterial diversity
total petroleum hydrocarbons
indigenous bacterial consortium
author_facet Yalda Basim
Ghasemali Mohebali
Sahand Jorfi
Ramin Nabizadeh
Mehdi Ahmadi Moghadam
Ata Ghadiri
Nematollah Jaafarzadeh Haghighi Fard
author_sort Yalda Basim
title Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
title_short Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
title_full Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
title_fullStr Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
title_full_unstemmed Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
title_sort biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
publisher Kerman University of Medical Sciences
series Environmental Health Engineering and Management
issn 2423-3765
2423-4311
publishDate 2020-06-01
description Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID). Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research. Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained.
topic oil-contaminated soils
biodegradation
bacterial diversity
total petroleum hydrocarbons
indigenous bacterial consortium
url http://ehemj.com/article-1-615-en.html
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AT ghasemalimohebali biodegradationoftotalpetroleumhydrocarbonsincontaminatedsoilsusingindigenousbacterialconsortium
AT sahandjorfi biodegradationoftotalpetroleumhydrocarbonsincontaminatedsoilsusingindigenousbacterialconsortium
AT raminnabizadeh biodegradationoftotalpetroleumhydrocarbonsincontaminatedsoilsusingindigenousbacterialconsortium
AT mehdiahmadimoghadam biodegradationoftotalpetroleumhydrocarbonsincontaminatedsoilsusingindigenousbacterialconsortium
AT ataghadiri biodegradationoftotalpetroleumhydrocarbonsincontaminatedsoilsusingindigenousbacterialconsortium
AT nematollahjaafarzadehhaghighifard biodegradationoftotalpetroleumhydrocarbonsincontaminatedsoilsusingindigenousbacterialconsortium
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