F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and blaCTX-M-55/-14/-65 in Escherichia coli from chickens in China
The purpose of this study was to examine the occurrence of fosfomycin-resistant Escherichia coli from chickens and to characterize the plasmids carrying fosA3. A total of 661 E. coli isolates of chicken origin collected from 2009 to 2011 were screened for plasmid-mediated fosfomycin resistance deter...
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doaj-ddaca9ec075f49c8812b5f3178d9f7b72020-11-24T22:13:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2014-12-01510.3389/fmicb.2014.00688112927F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and blaCTX-M-55/-14/-65 in Escherichia coli from chickens in ChinaXiaoyun eYang0Wuling eLiu1Yiyun eLiu2Jing eWang3Luchao eLv4Xiaojie eChen5Dandan eHe6Tong eYang7Jianxia eHou8Yinjuan eTan9Li eXing10Zhenling eZeng11Jian-Hua eLiu12South China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversityThe purpose of this study was to examine the occurrence of fosfomycin-resistant Escherichia coli from chickens and to characterize the plasmids carrying fosA3. A total of 661 E. coli isolates of chicken origin collected from 2009 to 2011 were screened for plasmid-mediated fosfomycin resistance determinants by PCR. Plasmids were characterized using PCR-based replicon typing, plasmid multilocus sequence typing, and restriction fragment length polymorphisms. Associated addiction systems and resistance genes were identified by PCR. PCR-mapping was used for analysis of the genetic context of fosA3. Fosfomycin resistance was detected in 58 isolates that also carried the fosA3 gene. Fifty-seven, 17, and 52 FosA3-producers also harbored blaCTX-M, rmtB, and floR genes, respectively. Most of the 58 fosA3-carrying isolates were clonally unrelated, and all fosA3 genes were located on plasmids belonged to F33:A-:B- (n=18), IncN-F33:A-:B- (n=7), IncHI2/ST3 (n=10), IncI1/ST71 (n=3), IncI1/ST108 (n=3), and others. The genetic structures, IS26-ISEcp1-blaCTX-M-55-orf477-blaTEM-1-IS26-fosA3-1758bp-IS26 and ISEcp1-blaCTX-M-65- IS903-iroN-IS26-fosA3-536bp-IS26 were located on highly similar F33:A-:B- plasmids. In addition, blaCTX-M-14-fosA3-IS26 was frequently present on similar IncHI2/ST3 plasmids. IncFII plasmids had a significantly higher frequency of addiction systems (mean 3.5) than other plasmids. Our results showed a surprisingly high prevalence of fosA3 gene in E. coli isolates recovered from chicken in China. The spread of fosA3 can be attributed to horizontal dissemination of several epidemic plasmids, especially F33:A-:B- plasmids. Since coselection by other antimicrobials is the major driving force for the diffusion of the fosA3 gene, a strict antibiotic use policy is urgently needed in China.http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00688/fullEscherichia coliFosfomycinPlasmidsPoultryCTX-Manimal reservoirs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoyun eYang Wuling eLiu Yiyun eLiu Jing eWang Luchao eLv Xiaojie eChen Dandan eHe Tong eYang Jianxia eHou Yinjuan eTan Li eXing Zhenling eZeng Jian-Hua eLiu |
spellingShingle |
Xiaoyun eYang Wuling eLiu Yiyun eLiu Jing eWang Luchao eLv Xiaojie eChen Dandan eHe Tong eYang Jianxia eHou Yinjuan eTan Li eXing Zhenling eZeng Jian-Hua eLiu F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and blaCTX-M-55/-14/-65 in Escherichia coli from chickens in China Frontiers in Microbiology Escherichia coli Fosfomycin Plasmids Poultry CTX-M animal reservoirs |
author_facet |
Xiaoyun eYang Wuling eLiu Yiyun eLiu Jing eWang Luchao eLv Xiaojie eChen Dandan eHe Tong eYang Jianxia eHou Yinjuan eTan Li eXing Zhenling eZeng Jian-Hua eLiu |
author_sort |
Xiaoyun eYang |
title |
F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and blaCTX-M-55/-14/-65 in Escherichia coli from chickens in China |
title_short |
F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and blaCTX-M-55/-14/-65 in Escherichia coli from chickens in China |
title_full |
F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and blaCTX-M-55/-14/-65 in Escherichia coli from chickens in China |
title_fullStr |
F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and blaCTX-M-55/-14/-65 in Escherichia coli from chickens in China |
title_full_unstemmed |
F33: A-: B-, IncHI2/ST3, and IncI1/ST71 plasmids drive the dissemination of fosA3 and blaCTX-M-55/-14/-65 in Escherichia coli from chickens in China |
title_sort |
f33: a-: b-, inchi2/st3, and inci1/st71 plasmids drive the dissemination of fosa3 and blactx-m-55/-14/-65 in escherichia coli from chickens in china |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2014-12-01 |
description |
The purpose of this study was to examine the occurrence of fosfomycin-resistant Escherichia coli from chickens and to characterize the plasmids carrying fosA3. A total of 661 E. coli isolates of chicken origin collected from 2009 to 2011 were screened for plasmid-mediated fosfomycin resistance determinants by PCR. Plasmids were characterized using PCR-based replicon typing, plasmid multilocus sequence typing, and restriction fragment length polymorphisms. Associated addiction systems and resistance genes were identified by PCR. PCR-mapping was used for analysis of the genetic context of fosA3. Fosfomycin resistance was detected in 58 isolates that also carried the fosA3 gene. Fifty-seven, 17, and 52 FosA3-producers also harbored blaCTX-M, rmtB, and floR genes, respectively. Most of the 58 fosA3-carrying isolates were clonally unrelated, and all fosA3 genes were located on plasmids belonged to F33:A-:B- (n=18), IncN-F33:A-:B- (n=7), IncHI2/ST3 (n=10), IncI1/ST71 (n=3), IncI1/ST108 (n=3), and others. The genetic structures, IS26-ISEcp1-blaCTX-M-55-orf477-blaTEM-1-IS26-fosA3-1758bp-IS26 and ISEcp1-blaCTX-M-65- IS903-iroN-IS26-fosA3-536bp-IS26 were located on highly similar F33:A-:B- plasmids. In addition, blaCTX-M-14-fosA3-IS26 was frequently present on similar IncHI2/ST3 plasmids. IncFII plasmids had a significantly higher frequency of addiction systems (mean 3.5) than other plasmids. Our results showed a surprisingly high prevalence of fosA3 gene in E. coli isolates recovered from chicken in China. The spread of fosA3 can be attributed to horizontal dissemination of several epidemic plasmids, especially F33:A-:B- plasmids. Since coselection by other antimicrobials is the major driving force for the diffusion of the fosA3 gene, a strict antibiotic use policy is urgently needed in China. |
topic |
Escherichia coli Fosfomycin Plasmids Poultry CTX-M animal reservoirs |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00688/full |
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