Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry.
To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are...
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2012-01-01
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doaj-ddbf2847645a4682b7eb857b51f154462020-11-25T02:20:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3851510.1371/journal.pone.0038515Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry.Kaveh EmamiVahid AskariMatthias UllrichKhwajah MohinudeenArga Chandrashekar AnilLidita KhandeparkerJ Grant BurgessEhsan MesbahiTo evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.http://europepmc.org/articles/PMC3369924?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kaveh Emami Vahid Askari Matthias Ullrich Khwajah Mohinudeen Arga Chandrashekar Anil Lidita Khandeparker J Grant Burgess Ehsan Mesbahi |
spellingShingle |
Kaveh Emami Vahid Askari Matthias Ullrich Khwajah Mohinudeen Arga Chandrashekar Anil Lidita Khandeparker J Grant Burgess Ehsan Mesbahi Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry. PLoS ONE |
author_facet |
Kaveh Emami Vahid Askari Matthias Ullrich Khwajah Mohinudeen Arga Chandrashekar Anil Lidita Khandeparker J Grant Burgess Ehsan Mesbahi |
author_sort |
Kaveh Emami |
title |
Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry. |
title_short |
Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry. |
title_full |
Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry. |
title_fullStr |
Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry. |
title_full_unstemmed |
Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry. |
title_sort |
characterization of bacteria in ballast water using maldi-tof mass spectrometry. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time. |
url |
http://europepmc.org/articles/PMC3369924?pdf=render |
work_keys_str_mv |
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