Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants
In this study, an attempt was made to investigate in vitro morphogenetic competence of three shrub species from the Thymelaeaceae family. The studied plant material originated from Russia, Greece and China, and the effectiveness of in vitro shoot formation and rhizogenesis of Daphne caucasica, D. ja...
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Polish Botanical Society
2012-12-01
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doaj-ddd567cf9b2c4d1b914588a7ddc1ba8a2020-11-25T03:07:49ZengPolish Botanical SocietyActa Agrobotanica2300-357X2012-12-01651212810.5586/aa.2012.0391015Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plantsEwa Hanus-Fajerska0Alina Wiszniewska1Przemysław Czaicki2Agricultural University of CracowAgricultural University of CracowAgricultural University of CracowIn this study, an attempt was made to investigate in vitro morphogenetic competence of three shrub species from the Thymelaeaceae family. The studied plant material originated from Russia, Greece and China, and the effectiveness of in vitro shoot formation and rhizogenesis of Daphne caucasica, D. jasminea, and D. tangutica was verified. The multiplication coefficient was compared for different propagation media. Medium composed of WPM mineral salts, MS microelements and a set of vitamins, supplemented with 1.0 mg dm-3 2iP, 0.1 mg dm-3 NAA, and 0.65 g dm-3 calcium gluconate, was appropriate for micropropagation of the tested genotypes. Shoot propagation in medium containing B5 vitamins and microelements was not as effective as on WPM/MS medium. The rooting phase, especially in D. tangutica, needs further optimization in order to reduce the costs associated with acclimatization of microplantlets obtained to in vivo conditions. After stabilization, the plants were successfully cultivated under greenhouse conditions.https://pbsociety.org.pl/journals/index.php/aa/article/view/1322regeneration capacitymicropropagationrooting efficacyacclimatizationDaphne sp. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ewa Hanus-Fajerska Alina Wiszniewska Przemysław Czaicki |
spellingShingle |
Ewa Hanus-Fajerska Alina Wiszniewska Przemysław Czaicki Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants Acta Agrobotanica regeneration capacity micropropagation rooting efficacy acclimatization Daphne sp. |
author_facet |
Ewa Hanus-Fajerska Alina Wiszniewska Przemysław Czaicki |
author_sort |
Ewa Hanus-Fajerska |
title |
Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants |
title_short |
Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants |
title_full |
Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants |
title_fullStr |
Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants |
title_full_unstemmed |
Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants |
title_sort |
effectiveness of daphne l. (thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants |
publisher |
Polish Botanical Society |
series |
Acta Agrobotanica |
issn |
2300-357X |
publishDate |
2012-12-01 |
description |
In this study, an attempt was made to investigate in vitro morphogenetic competence of three shrub species from the Thymelaeaceae family. The studied plant material originated from Russia, Greece and China, and the effectiveness of in vitro shoot formation and rhizogenesis of Daphne caucasica, D. jasminea, and D. tangutica was verified. The multiplication coefficient was compared for different propagation media. Medium composed of WPM mineral salts, MS microelements and a set of vitamins, supplemented with 1.0 mg dm-3 2iP, 0.1 mg dm-3 NAA, and 0.65 g dm-3 calcium gluconate, was appropriate for micropropagation of the tested genotypes. Shoot propagation in medium containing B5 vitamins and microelements was not as effective as on WPM/MS medium. The rooting phase, especially in D. tangutica, needs further optimization in order to reduce the costs associated with acclimatization of microplantlets obtained to in vivo conditions. After stabilization, the plants were successfully cultivated under greenhouse conditions. |
topic |
regeneration capacity micropropagation rooting efficacy acclimatization Daphne sp. |
url |
https://pbsociety.org.pl/journals/index.php/aa/article/view/1322 |
work_keys_str_mv |
AT ewahanusfajerska effectivenessofdaphnelthymelaeaceaeinvitropropagationrootingofmicroshootsandacclimatizationofplants AT alinawiszniewska effectivenessofdaphnelthymelaeaceaeinvitropropagationrootingofmicroshootsandacclimatizationofplants AT przemysławczaicki effectivenessofdaphnelthymelaeaceaeinvitropropagationrootingofmicroshootsandacclimatizationofplants |
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1724668983387357184 |