Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced aging
Copper (Cu) pollution in water and agricultural soil has always been a worldwide concern. This research aims to investigate the health effects of copper exposure on Caenorhabditis elegans (C. elegans) under the existing environmental quality standards (1 mg/L and 2 mg/L) via lifespan, reproduction,...
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doaj-dde301fe92d9418b9c2fcfa1e9def12a2021-08-02T04:38:27ZengElsevierEcotoxicology and Environmental Safety0147-65132021-10-01222112494Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced agingYing Zhang0Chao Zhao1Hu Zhang2Ran Liu3Shizhi Wang4Yuepu Pu5Lihong Yin6Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, ChinaKey Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, ChinaKey Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, ChinaKey Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, ChinaKey Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, ChinaKey Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, ChinaCorrespondence to: School of Public Health, Southeast University, 87 Dingjiaqiao Street, Nanjing 210009, China.; Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, ChinaCopper (Cu) pollution in water and agricultural soil has always been a worldwide concern. This research aims to investigate the health effects of copper exposure on Caenorhabditis elegans (C. elegans) under the existing environmental quality standards (1 mg/L and 2 mg/L) via lifespan, reproduction, biological markers and transcriptome analysis. The results showed that copper of these two environmental standards shorten the lifespan of nematodes, reduced the brood size, reduced the frequency of pharyngeal pumps and prolonged defecation time as aging-related behaviors, and increased the levels of aging-related markers ROS, MDA and H2O2. There was a certain effect trend for the two exposure concentrations. Further, the possible molecular mechanism of copper-induced aging and reproductive effects on C. elegans was explored. Differential gene expression analysis was performed, and 2332 genes (567 up- and 1765 down-regulated genes) in the 1 mg/L group, 2449 DEGs (724 up- and 1725 down-regulated genes) in the 2 mg/L group in response to copper treatment. The top 20 regulated genes were vit (vit-1, vit-3, vit-4) genes, col genes (col-35, col-72, col-114, col-123, col-164, col-183, col-185), eea-1, him-18 and grl-20, which suggested that cuticle collagen synthesis and yolk expression were disrupted by copper. Analysis of KEGG pathway showed copper exposure widely affects longevity regulation pathways, thereby promoting aging. In summary, the sequencing results extensively and deeply reveal the health hazards of environmentally relevant doses of copper exposure to C. elegans, and behavioral testing verified that copper promoted aging of C. elegans.http://www.sciencedirect.com/science/article/pii/S0147651321006060Copper-induced agingLifespanCaenorhabditis elegansDifferentially expressed genes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ying Zhang Chao Zhao Hu Zhang Ran Liu Shizhi Wang Yuepu Pu Lihong Yin |
spellingShingle |
Ying Zhang Chao Zhao Hu Zhang Ran Liu Shizhi Wang Yuepu Pu Lihong Yin Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced aging Ecotoxicology and Environmental Safety Copper-induced aging Lifespan Caenorhabditis elegans Differentially expressed genes |
author_facet |
Ying Zhang Chao Zhao Hu Zhang Ran Liu Shizhi Wang Yuepu Pu Lihong Yin |
author_sort |
Ying Zhang |
title |
Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced aging |
title_short |
Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced aging |
title_full |
Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced aging |
title_fullStr |
Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced aging |
title_full_unstemmed |
Integrating transcriptomics and behavior tests reveals how the C. elegans responds to copper induced aging |
title_sort |
integrating transcriptomics and behavior tests reveals how the c. elegans responds to copper induced aging |
publisher |
Elsevier |
series |
Ecotoxicology and Environmental Safety |
issn |
0147-6513 |
publishDate |
2021-10-01 |
description |
Copper (Cu) pollution in water and agricultural soil has always been a worldwide concern. This research aims to investigate the health effects of copper exposure on Caenorhabditis elegans (C. elegans) under the existing environmental quality standards (1 mg/L and 2 mg/L) via lifespan, reproduction, biological markers and transcriptome analysis. The results showed that copper of these two environmental standards shorten the lifespan of nematodes, reduced the brood size, reduced the frequency of pharyngeal pumps and prolonged defecation time as aging-related behaviors, and increased the levels of aging-related markers ROS, MDA and H2O2. There was a certain effect trend for the two exposure concentrations. Further, the possible molecular mechanism of copper-induced aging and reproductive effects on C. elegans was explored. Differential gene expression analysis was performed, and 2332 genes (567 up- and 1765 down-regulated genes) in the 1 mg/L group, 2449 DEGs (724 up- and 1725 down-regulated genes) in the 2 mg/L group in response to copper treatment. The top 20 regulated genes were vit (vit-1, vit-3, vit-4) genes, col genes (col-35, col-72, col-114, col-123, col-164, col-183, col-185), eea-1, him-18 and grl-20, which suggested that cuticle collagen synthesis and yolk expression were disrupted by copper. Analysis of KEGG pathway showed copper exposure widely affects longevity regulation pathways, thereby promoting aging. In summary, the sequencing results extensively and deeply reveal the health hazards of environmentally relevant doses of copper exposure to C. elegans, and behavioral testing verified that copper promoted aging of C. elegans. |
topic |
Copper-induced aging Lifespan Caenorhabditis elegans Differentially expressed genes |
url |
http://www.sciencedirect.com/science/article/pii/S0147651321006060 |
work_keys_str_mv |
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