High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo
The present study investigated the transcriptomic response of porcine dendritic cells (DC) to innate stimulation in vitro and in vivo. The aim was to identify DC subset-specialization, suitable Toll-like receptor (TLR) ligands targeting plasmacytoid DC (pDC), and the DC activation profile during hig...
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doaj-ddeee9c6c0ad410db58641c88a3721f52020-11-25T03:04:15ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-07-011110.3389/fimmu.2020.01429548859High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivoGaël Auray0Gaël Auray1Stephanie C. Talker2Stephanie C. Talker3Irene Keller4Sylvie Python5Markus Gerber6Matthias Liniger7Matthias Liniger8Llilianne Ganges9Rémy Bruggmann10Nicolas Ruggli11Nicolas Ruggli12Artur Summerfield13Artur Summerfield14Institute of Virology and Immunology, Mittelhäusern, SwitzerlandDepartment of Infectious Diseases and Pathobiology, University of Bern, Bern, SwitzerlandInstitute of Virology and Immunology, Mittelhäusern, SwitzerlandDepartment of Infectious Diseases and Pathobiology, University of Bern, Bern, SwitzerlandDepartment for Biomedical Research and Swiss Institute of Bioinformatics, University of Bern, Bern, SwitzerlandInstitute of Virology and Immunology, Mittelhäusern, SwitzerlandInstitute of Virology and Immunology, Mittelhäusern, SwitzerlandInstitute of Virology and Immunology, Mittelhäusern, SwitzerlandDepartment of Infectious Diseases and Pathobiology, University of Bern, Bern, SwitzerlandOIE Reference Laboratory for Classical Swine Fever, IRTA-CReSA, Barcelona, SpainInterfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, Bern, SwitzerlandInstitute of Virology and Immunology, Mittelhäusern, SwitzerlandDepartment of Infectious Diseases and Pathobiology, University of Bern, Bern, SwitzerlandInstitute of Virology and Immunology, Mittelhäusern, SwitzerlandDepartment of Infectious Diseases and Pathobiology, University of Bern, Bern, SwitzerlandThe present study investigated the transcriptomic response of porcine dendritic cells (DC) to innate stimulation in vitro and in vivo. The aim was to identify DC subset-specialization, suitable Toll-like receptor (TLR) ligands targeting plasmacytoid DC (pDC), and the DC activation profile during highly and low virulent classical swine fever virus (CSFV, strain Eystrup and Pinar del Rio, respectively) infection, chosen as model for a virus causing a severe immunopathology. After identification of porcine conventional DC (cDC) 1, cDC2, pDC and a monocyte-derived subset in lymphoid tissues, we characterized DC activation using transcriptomics, and focused on chemokines, interferons, cytokines, as well as on co-stimulatory and inhibitory molecules. We demonstrate that porcine pDC provide important signals for Th1 and interferon responses, with CpG triggering the strongest responses in pDC. DC isolated early after infection of pigs with either of the two CSFV strains showed prominent upregulation of CCL5, CXCL9, CXCL10, CXCL11, and XCL1, as well as of the cytokines TNFSF13B, IL6, IL7, IL12B, IL15, IL27. Transcription of IL12B and many interferon genes were mostly restricted to pDC. Interestingly, the infection was associated with a prominent induction of inhibitory and cell death receptors. When comparing low and highly virulent CSFV strains, the latter induced a stronger inflammatory and antiviral response but a weaker cell cycle response, and reduced antigen presentation functions of DC. Taken together, we provide high-resolution information on DC activation in pigs, as well as information on how DC modulation could be linked to CSFV immunopathology.https://www.frontiersin.org/article/10.3389/fimmu.2020.01429/fulldendritic cellstoll like receptortranscriptomics analysisporcine (pig) modelclassical swine fever |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gaël Auray Gaël Auray Stephanie C. Talker Stephanie C. Talker Irene Keller Sylvie Python Markus Gerber Matthias Liniger Matthias Liniger Llilianne Ganges Rémy Bruggmann Nicolas Ruggli Nicolas Ruggli Artur Summerfield Artur Summerfield |
spellingShingle |
Gaël Auray Gaël Auray Stephanie C. Talker Stephanie C. Talker Irene Keller Sylvie Python Markus Gerber Matthias Liniger Matthias Liniger Llilianne Ganges Rémy Bruggmann Nicolas Ruggli Nicolas Ruggli Artur Summerfield Artur Summerfield High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo Frontiers in Immunology dendritic cells toll like receptor transcriptomics analysis porcine (pig) model classical swine fever |
author_facet |
Gaël Auray Gaël Auray Stephanie C. Talker Stephanie C. Talker Irene Keller Sylvie Python Markus Gerber Matthias Liniger Matthias Liniger Llilianne Ganges Rémy Bruggmann Nicolas Ruggli Nicolas Ruggli Artur Summerfield Artur Summerfield |
author_sort |
Gaël Auray |
title |
High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo |
title_short |
High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo |
title_full |
High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo |
title_fullStr |
High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo |
title_full_unstemmed |
High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo |
title_sort |
high-resolution profiling of innate immune responses by porcine dendritic cell subsets in vitro and in vivo |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2020-07-01 |
description |
The present study investigated the transcriptomic response of porcine dendritic cells (DC) to innate stimulation in vitro and in vivo. The aim was to identify DC subset-specialization, suitable Toll-like receptor (TLR) ligands targeting plasmacytoid DC (pDC), and the DC activation profile during highly and low virulent classical swine fever virus (CSFV, strain Eystrup and Pinar del Rio, respectively) infection, chosen as model for a virus causing a severe immunopathology. After identification of porcine conventional DC (cDC) 1, cDC2, pDC and a monocyte-derived subset in lymphoid tissues, we characterized DC activation using transcriptomics, and focused on chemokines, interferons, cytokines, as well as on co-stimulatory and inhibitory molecules. We demonstrate that porcine pDC provide important signals for Th1 and interferon responses, with CpG triggering the strongest responses in pDC. DC isolated early after infection of pigs with either of the two CSFV strains showed prominent upregulation of CCL5, CXCL9, CXCL10, CXCL11, and XCL1, as well as of the cytokines TNFSF13B, IL6, IL7, IL12B, IL15, IL27. Transcription of IL12B and many interferon genes were mostly restricted to pDC. Interestingly, the infection was associated with a prominent induction of inhibitory and cell death receptors. When comparing low and highly virulent CSFV strains, the latter induced a stronger inflammatory and antiviral response but a weaker cell cycle response, and reduced antigen presentation functions of DC. Taken together, we provide high-resolution information on DC activation in pigs, as well as information on how DC modulation could be linked to CSFV immunopathology. |
topic |
dendritic cells toll like receptor transcriptomics analysis porcine (pig) model classical swine fever |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2020.01429/full |
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