Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation
Abstract Cigar tobacco leaves (CTLs) contain abundant bacteria and fungi that are vital to leaf quality during fermentation. In this study, artificial fermentation was used for the fermentation of CTLs since it was more controllable and efficient than natural aging. The bacterial and fungal communit...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wiley
2021-04-01
|
Series: | MicrobiologyOpen |
Subjects: | |
Online Access: | https://doi.org/10.1002/mbo3.1171 |
id |
doaj-de01a6924517451b938b14eacac61b4d |
---|---|
record_format |
Article |
spelling |
doaj-de01a6924517451b938b14eacac61b4d2021-05-15T00:45:46ZengWileyMicrobiologyOpen2045-88272021-04-01102n/an/a10.1002/mbo3.1171Microbial community and metabolic function analysis of cigar tobacco leaves during fermentationFang Liu0Zhiyong Wu1Xiaoping Zhang2Gaolei Xi3Zhe Zhao4Miao Lai5Mingqin Zhao6College of Tobacco Science Henan Agricultural University Zhengzhou ChinaCollege of Tobacco Science Henan Agricultural University Zhengzhou ChinaCollege of Tobacco Science Henan Agricultural University Zhengzhou ChinaChina Tobacco Henan Ind Co Ltd, Ctr Technol Zhengzhou ChinaCollege of Tobacco Science Henan Agricultural University Zhengzhou ChinaCollege of Tobacco Science Henan Agricultural University Zhengzhou ChinaCollege of Tobacco Science Henan Agricultural University Zhengzhou ChinaAbstract Cigar tobacco leaves (CTLs) contain abundant bacteria and fungi that are vital to leaf quality during fermentation. In this study, artificial fermentation was used for the fermentation of CTLs since it was more controllable and efficient than natural aging. The bacterial and fungal community structure and composition in unfermented and fermented CTLs were determined to understand the effects of microbes on the characteristics of CTLs during artificial fermentation. The relationship between the chemical contents and alterations in the microbial composition was evaluated, and the functions of bacteria and fungi in fermented CTLs were predicted to determine the possible metabolic pathways. After artificial fermentation, the bacterial and fungal community structure significantly changed in CTLs. The total nitrate and nicotine contents were most readily affected by the bacterial and fungal communities, respectively. FAPROTAX software predictions of the bacterial community revealed increases in functions related to compound transformation after fermentation. FUNGuild predictions of the fungal community revealed an increase in the content of saprotrophic fungi after fermentation. These data provide information regarding the artificial fermentation mechanism of CTLs and will inform safety and quality improvements.https://doi.org/10.1002/mbo3.1171artificial fermentationchemical compositionscigar tobacco leavesfunction predictionmicrobial community |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fang Liu Zhiyong Wu Xiaoping Zhang Gaolei Xi Zhe Zhao Miao Lai Mingqin Zhao |
spellingShingle |
Fang Liu Zhiyong Wu Xiaoping Zhang Gaolei Xi Zhe Zhao Miao Lai Mingqin Zhao Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation MicrobiologyOpen artificial fermentation chemical compositions cigar tobacco leaves function prediction microbial community |
author_facet |
Fang Liu Zhiyong Wu Xiaoping Zhang Gaolei Xi Zhe Zhao Miao Lai Mingqin Zhao |
author_sort |
Fang Liu |
title |
Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation |
title_short |
Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation |
title_full |
Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation |
title_fullStr |
Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation |
title_full_unstemmed |
Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation |
title_sort |
microbial community and metabolic function analysis of cigar tobacco leaves during fermentation |
publisher |
Wiley |
series |
MicrobiologyOpen |
issn |
2045-8827 |
publishDate |
2021-04-01 |
description |
Abstract Cigar tobacco leaves (CTLs) contain abundant bacteria and fungi that are vital to leaf quality during fermentation. In this study, artificial fermentation was used for the fermentation of CTLs since it was more controllable and efficient than natural aging. The bacterial and fungal community structure and composition in unfermented and fermented CTLs were determined to understand the effects of microbes on the characteristics of CTLs during artificial fermentation. The relationship between the chemical contents and alterations in the microbial composition was evaluated, and the functions of bacteria and fungi in fermented CTLs were predicted to determine the possible metabolic pathways. After artificial fermentation, the bacterial and fungal community structure significantly changed in CTLs. The total nitrate and nicotine contents were most readily affected by the bacterial and fungal communities, respectively. FAPROTAX software predictions of the bacterial community revealed increases in functions related to compound transformation after fermentation. FUNGuild predictions of the fungal community revealed an increase in the content of saprotrophic fungi after fermentation. These data provide information regarding the artificial fermentation mechanism of CTLs and will inform safety and quality improvements. |
topic |
artificial fermentation chemical compositions cigar tobacco leaves function prediction microbial community |
url |
https://doi.org/10.1002/mbo3.1171 |
work_keys_str_mv |
AT fangliu microbialcommunityandmetabolicfunctionanalysisofcigartobaccoleavesduringfermentation AT zhiyongwu microbialcommunityandmetabolicfunctionanalysisofcigartobaccoleavesduringfermentation AT xiaopingzhang microbialcommunityandmetabolicfunctionanalysisofcigartobaccoleavesduringfermentation AT gaoleixi microbialcommunityandmetabolicfunctionanalysisofcigartobaccoleavesduringfermentation AT zhezhao microbialcommunityandmetabolicfunctionanalysisofcigartobaccoleavesduringfermentation AT miaolai microbialcommunityandmetabolicfunctionanalysisofcigartobaccoleavesduringfermentation AT mingqinzhao microbialcommunityandmetabolicfunctionanalysisofcigartobaccoleavesduringfermentation |
_version_ |
1721440774576406528 |