α-Glucosidase Inhibitors from Vauquelinia corymbosa
The α-glucosidase inhibitory activity of an aqueous extract and compounds from the aerial parts of V. corymbosa was demonstrated with yeast and rat small intestinal α-glucosidases. The aqueous extract inhibited yeast α-glucosidase with a half maximal inhibitory concentration (IC50) of 28.6 μg/mL....
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doaj-de04968a87d848b0ac2ef22ce7075a292020-11-24T22:13:55ZengMDPI AGMolecules1420-30492015-08-01208153301534210.3390/molecules200815330molecules200815330α-Glucosidase Inhibitors from Vauquelinia corymbosaLaura Flores-Bocanegra0Araceli Pérez-Vásquez1Mariana Torres-Piedra2Robert Bye3Edelmira Linares4Rachel Mata5Facultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoFacultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoFacultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoInstituto de Biología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoInstituto de Biología, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoFacultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoThe α-glucosidase inhibitory activity of an aqueous extract and compounds from the aerial parts of V. corymbosa was demonstrated with yeast and rat small intestinal α-glucosidases. The aqueous extract inhibited yeast α-glucosidase with a half maximal inhibitory concentration (IC50) of 28.6 μg/mL. Bioassay-guided fractionation of the extract led to the isolation of several compounds, including one cyanogenic glycoside [prunasin (1)], five flavonoids [(−)-epi-catechin (2), hyperoside (3), isoquercetin (4), quercitrin (5) and quercetin-3-O-(6′′-benzoyl)-β-galactoside (6)] and two simple aromatic compounds [picein (7) and methylarbutin (8)]. The most active compound was 6 with IC50 values of 30 μM in the case of yeast α-glucosidase, and 437 μM in the case of the mammalian enzyme. According to the kinetic analyses performed with rat and yeast enzymes, this compound behaved as mixed-type inhibitor; the calculated inhibition constants (Ki) were 212 and 50 μM, respectively. Molecular docking analyses with yeast and mammalian α-glucosidases revealed that compound 6 bind differently to these enzymes. Altogether, the results of this work suggest that preparations of V. corymbosa might delay glucose absorption in vivo.http://www.mdpi.com/1420-3049/20/8/15330diabetesyeast and rat α-glucosidasesVauquelinia corymbosa |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laura Flores-Bocanegra Araceli Pérez-Vásquez Mariana Torres-Piedra Robert Bye Edelmira Linares Rachel Mata |
spellingShingle |
Laura Flores-Bocanegra Araceli Pérez-Vásquez Mariana Torres-Piedra Robert Bye Edelmira Linares Rachel Mata α-Glucosidase Inhibitors from Vauquelinia corymbosa Molecules diabetes yeast and rat α-glucosidases Vauquelinia corymbosa |
author_facet |
Laura Flores-Bocanegra Araceli Pérez-Vásquez Mariana Torres-Piedra Robert Bye Edelmira Linares Rachel Mata |
author_sort |
Laura Flores-Bocanegra |
title |
α-Glucosidase Inhibitors from Vauquelinia corymbosa |
title_short |
α-Glucosidase Inhibitors from Vauquelinia corymbosa |
title_full |
α-Glucosidase Inhibitors from Vauquelinia corymbosa |
title_fullStr |
α-Glucosidase Inhibitors from Vauquelinia corymbosa |
title_full_unstemmed |
α-Glucosidase Inhibitors from Vauquelinia corymbosa |
title_sort |
α-glucosidase inhibitors from vauquelinia corymbosa |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2015-08-01 |
description |
The α-glucosidase inhibitory activity of an aqueous extract and compounds from the aerial parts of V. corymbosa was demonstrated with yeast and rat small intestinal α-glucosidases. The aqueous extract inhibited yeast α-glucosidase with a half maximal inhibitory concentration (IC50) of 28.6 μg/mL. Bioassay-guided fractionation of the extract led to the isolation of several compounds, including one cyanogenic glycoside [prunasin (1)], five flavonoids [(−)-epi-catechin (2), hyperoside (3), isoquercetin (4), quercitrin (5) and quercetin-3-O-(6′′-benzoyl)-β-galactoside (6)] and two simple aromatic compounds [picein (7) and methylarbutin (8)]. The most active compound was 6 with IC50 values of 30 μM in the case of yeast α-glucosidase, and 437 μM in the case of the mammalian enzyme. According to the kinetic analyses performed with rat and yeast enzymes, this compound behaved as mixed-type inhibitor; the calculated inhibition constants (Ki) were 212 and 50 μM, respectively. Molecular docking analyses with yeast and mammalian α-glucosidases revealed that compound 6 bind differently to these enzymes. Altogether, the results of this work suggest that preparations of V. corymbosa might delay glucose absorption in vivo. |
topic |
diabetes yeast and rat α-glucosidases Vauquelinia corymbosa |
url |
http://www.mdpi.com/1420-3049/20/8/15330 |
work_keys_str_mv |
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