Parecoxib improves atherosclerotic plaque stability by suppressing inflammation and inhibiting matrix metalloproteinases production
With the aging population, coronary syndrome is one of the leading causes of mortality. Atherosclerosis is the pathophysiological basis of coronary syndrome, which is caused by plaque rupture and predisposed or aggravated by many perioperative complications. Parecoxib is one of the most widely used...
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doaj-de093428fac24fed9fcdfa103ce345e22021-05-20T07:44:58ZengElsevierBiomedicine & Pharmacotherapy0753-33222021-06-01138111423Parecoxib improves atherosclerotic plaque stability by suppressing inflammation and inhibiting matrix metalloproteinases productionChao Gong0Yu Qi1Yang Xu2Xiruo Tang3Feng Liang4Lianhua Chen5Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, ChinaDepartment of Cardiology, Nanjing Drum Tower Hospital, State Key Laboratory of Pharmaceutical Biotechnology, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, ChinaDepartment of Thoracic Surgery, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, ChinaShanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaShanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaShanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China; Corresponding author.With the aging population, coronary syndrome is one of the leading causes of mortality. Atherosclerosis is the pathophysiological basis of coronary syndrome, which is caused by plaque rupture and predisposed or aggravated by many perioperative complications. Parecoxib is one of the most widely used nonsteroidal anti-inflammatory perioperative drugs. This study aims to evaluate the potential benefits of parecoxib on atherosclerosis progression. Apolipoprotein E-deficient (Apo E-/-) mice were intraperitoneally injected by parecoxib (par group) or saline (control group) and, meanwhile, were given a western diet for 12 weeks. The aorta and aortic root were examined by oil red O (ORO) staining for atherosclerotic lesions. The expression level of matrix metalloproteinases (MMPs), was investigated using immunofluorescence and western blot. Macrophage inflammation was investigated by Q-PCR. Parecoxib treatment increased the number of vascular smooth muscle cells (VSMC) and amount of collagen, while and decreased the number of macrophages in murine aortic walls. The expression of MMP1, 2, 9, and 13 as well as IL- 1β and IL-6 were also decreased in the par group. However, there was no statistical difference in lipid infiltration between the two groups. Parecoxib could improve plaque stability by suppressing inflammation and inhibiting MMPs production.http://www.sciencedirect.com/science/article/pii/S0753332221002080ParecoxibAtherosclerotic plaqueMatrix metalloproteinasesOil red O |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chao Gong Yu Qi Yang Xu Xiruo Tang Feng Liang Lianhua Chen |
spellingShingle |
Chao Gong Yu Qi Yang Xu Xiruo Tang Feng Liang Lianhua Chen Parecoxib improves atherosclerotic plaque stability by suppressing inflammation and inhibiting matrix metalloproteinases production Biomedicine & Pharmacotherapy Parecoxib Atherosclerotic plaque Matrix metalloproteinases Oil red O |
author_facet |
Chao Gong Yu Qi Yang Xu Xiruo Tang Feng Liang Lianhua Chen |
author_sort |
Chao Gong |
title |
Parecoxib improves atherosclerotic plaque stability by suppressing inflammation and inhibiting matrix metalloproteinases production |
title_short |
Parecoxib improves atherosclerotic plaque stability by suppressing inflammation and inhibiting matrix metalloproteinases production |
title_full |
Parecoxib improves atherosclerotic plaque stability by suppressing inflammation and inhibiting matrix metalloproteinases production |
title_fullStr |
Parecoxib improves atherosclerotic plaque stability by suppressing inflammation and inhibiting matrix metalloproteinases production |
title_full_unstemmed |
Parecoxib improves atherosclerotic plaque stability by suppressing inflammation and inhibiting matrix metalloproteinases production |
title_sort |
parecoxib improves atherosclerotic plaque stability by suppressing inflammation and inhibiting matrix metalloproteinases production |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2021-06-01 |
description |
With the aging population, coronary syndrome is one of the leading causes of mortality. Atherosclerosis is the pathophysiological basis of coronary syndrome, which is caused by plaque rupture and predisposed or aggravated by many perioperative complications. Parecoxib is one of the most widely used nonsteroidal anti-inflammatory perioperative drugs. This study aims to evaluate the potential benefits of parecoxib on atherosclerosis progression. Apolipoprotein E-deficient (Apo E-/-) mice were intraperitoneally injected by parecoxib (par group) or saline (control group) and, meanwhile, were given a western diet for 12 weeks. The aorta and aortic root were examined by oil red O (ORO) staining for atherosclerotic lesions. The expression level of matrix metalloproteinases (MMPs), was investigated using immunofluorescence and western blot. Macrophage inflammation was investigated by Q-PCR. Parecoxib treatment increased the number of vascular smooth muscle cells (VSMC) and amount of collagen, while and decreased the number of macrophages in murine aortic walls. The expression of MMP1, 2, 9, and 13 as well as IL- 1β and IL-6 were also decreased in the par group. However, there was no statistical difference in lipid infiltration between the two groups. Parecoxib could improve plaque stability by suppressing inflammation and inhibiting MMPs production. |
topic |
Parecoxib Atherosclerotic plaque Matrix metalloproteinases Oil red O |
url |
http://www.sciencedirect.com/science/article/pii/S0753332221002080 |
work_keys_str_mv |
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