Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing

A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the micro...

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Main Authors:	Weiming Wang, Hang Liu, Yan Yu, Fengyu Cong, Jun Yu
Format:	Article
Language:	English
Published:	MDPI AG 2020-04-01
Series:	Sensors
Subjects:	FOA lens yeast activity yeast concentration portable microscope
Online Access:	https://www.mdpi.com/1424-8220/20/7/2092

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record_format	Article
spelling	doaj-de177a623df54836bf15b11caafc5b162020-11-25T02:43:22ZengMDPI AGSensors1424-82202020-04-01202092209210.3390/s20072092Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image ProcessingWeiming Wang0Hang Liu1Yan Yu2Fengyu Cong3Jun Yu4Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian 116024, ChinaFaculty of Electronic Information and Electrical Engineering, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian 116024, ChinaFaculty of Electronic Information and Electrical Engineering, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian 116024, ChinaFaculty of Electronic Information and Electrical Engineering, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian 116024, ChinaFaculty of Electronic Information and Electrical Engineering, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian 116024, ChinaA fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the microscope based on the FOA. To further validate the FOA microscope’s imaging capability, yeast activity and concentration were investigated by simple image processing. The results showed that the percentages of live and dead yeast cells correctly identified were 92.1% and 84.8%, except for the clusters, which agreed well with the manual counting methods. Then, the performances of the portable microscopes based on the FOA and lens were compared and the factors that affect the FOA microscope imaging performance were analyzed.https://www.mdpi.com/1424-8220/20/7/2092FOAlensyeast activityyeast concentrationportable microscope
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Weiming Wang Hang Liu Yan Yu Fengyu Cong Jun Yu
spellingShingle	Weiming Wang Hang Liu Yan Yu Fengyu Cong Jun Yu Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing Sensors FOA lens yeast activity yeast concentration portable microscope
author_facet	Weiming Wang Hang Liu Yan Yu Fengyu Cong Jun Yu
author_sort	Weiming Wang
title	Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title_short	Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title_full	Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title_fullStr	Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title_full_unstemmed	Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title_sort	rapid yeast cell viability analysis by using a portable microscope based on the fiber optic array and simple image processing
publisher	MDPI AG
series	Sensors
issn	1424-8220
publishDate	2020-04-01
description	A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the microscope based on the FOA. To further validate the FOA microscope’s imaging capability, yeast activity and concentration were investigated by simple image processing. The results showed that the percentages of live and dead yeast cells correctly identified were 92.1% and 84.8%, except for the clusters, which agreed well with the manual counting methods. Then, the performances of the portable microscopes based on the FOA and lens were compared and the factors that affect the FOA microscope imaging performance were analyzed.
topic	FOA lens yeast activity yeast concentration portable microscope
url	https://www.mdpi.com/1424-8220/20/7/2092
work_keys_str_mv	AT weimingwang rapidyeastcellviabilityanalysisbyusingaportablemicroscopebasedonthefiberopticarrayandsimpleimageprocessing AT hangliu rapidyeastcellviabilityanalysisbyusingaportablemicroscopebasedonthefiberopticarrayandsimpleimageprocessing AT yanyu rapidyeastcellviabilityanalysisbyusingaportablemicroscopebasedonthefiberopticarrayandsimpleimageprocessing AT fengyucong rapidyeastcellviabilityanalysisbyusingaportablemicroscopebasedonthefiberopticarrayandsimpleimageprocessing AT junyu rapidyeastcellviabilityanalysisbyusingaportablemicroscopebasedonthefiberopticarrayandsimpleimageprocessing
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Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing

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