Molecular characterization of two type I acyl-CoA: diacylglycerol acyltransferase genes in maize
Type I acyl-CoA: diacylglycerol acyltransferase (DGAT1) is a rate-limiting enzyme that catalyses the final step of the Kennedy pathway in triacylglycerol biosynthesis. In this study, two genes encoding type I diacylglycerol acyltransferase, ZmDGAT1a and ZmDGAT1b, were cloned from a high-oil maize in...
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2016-05-01
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doaj-de2c4f491f584e788de2b1ae46cb044d2020-11-25T01:15:34ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302016-05-0130345346110.1080/13102818.2016.11570361157036Molecular characterization of two type I acyl-CoA: diacylglycerol acyltransferase genes in maizeQingzhi Li0Lizong Hu1Jinjie Guo2Tongwen Yang3Long Chen4Zhoukou Normal UniversityZhoukou Normal UniversityHebei Agricultural UniversityZhoukou Normal UniversityZhoukou Normal UniversityType I acyl-CoA: diacylglycerol acyltransferase (DGAT1) is a rate-limiting enzyme that catalyses the final step of the Kennedy pathway in triacylglycerol biosynthesis. In this study, two genes encoding type I diacylglycerol acyltransferase, ZmDGAT1a and ZmDGAT1b, were cloned from a high-oil maize inbred line and confirmed by Southern blotting. The complete coding sequences were obtained. Comparison between genomic DNA and coding sequences revealed that both ZmDGAT1a and ZmDGAT1b comprised 16 exons and 15 introns with a coding region of 1485 and 1488 bp, respectively. ZmDGAT1a and ZmDGAT1b shared similar molecular mass, isoelectric point, and transmembrane domains. ZmDGAT1b was expressed in all sampled tissues that were examined by using reverse transcription-polymerase chain reaction (RT-PCR). In contrast, ZmDGAT1a expression was limited to the silks, tassels, and seeds of different ages. This suggests that the two genes do not have a complementary relationship. The ZmDGAT1a and ZmDGAT1b proteins were subsequently found to be localized in the endoplasmic reticulum (ER). The data provides an important basis for further studies of the functions of DGAT1 genes and for genetic modification of the oil content and composition of corn seeds.http://dx.doi.org/10.1080/13102818.2016.1157036maizediacylglycerol acyltransferaseexpression patternevolutiongene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qingzhi Li Lizong Hu Jinjie Guo Tongwen Yang Long Chen |
spellingShingle |
Qingzhi Li Lizong Hu Jinjie Guo Tongwen Yang Long Chen Molecular characterization of two type I acyl-CoA: diacylglycerol acyltransferase genes in maize Biotechnology & Biotechnological Equipment maize diacylglycerol acyltransferase expression pattern evolution gene |
author_facet |
Qingzhi Li Lizong Hu Jinjie Guo Tongwen Yang Long Chen |
author_sort |
Qingzhi Li |
title |
Molecular characterization of two type I acyl-CoA: diacylglycerol acyltransferase genes in maize |
title_short |
Molecular characterization of two type I acyl-CoA: diacylglycerol acyltransferase genes in maize |
title_full |
Molecular characterization of two type I acyl-CoA: diacylglycerol acyltransferase genes in maize |
title_fullStr |
Molecular characterization of two type I acyl-CoA: diacylglycerol acyltransferase genes in maize |
title_full_unstemmed |
Molecular characterization of two type I acyl-CoA: diacylglycerol acyltransferase genes in maize |
title_sort |
molecular characterization of two type i acyl-coa: diacylglycerol acyltransferase genes in maize |
publisher |
Taylor & Francis Group |
series |
Biotechnology & Biotechnological Equipment |
issn |
1310-2818 1314-3530 |
publishDate |
2016-05-01 |
description |
Type I acyl-CoA: diacylglycerol acyltransferase (DGAT1) is a rate-limiting enzyme that catalyses the final step of the Kennedy pathway in triacylglycerol biosynthesis. In this study, two genes encoding type I diacylglycerol acyltransferase, ZmDGAT1a and ZmDGAT1b, were cloned from a high-oil maize inbred line and confirmed by Southern blotting. The complete coding sequences were obtained. Comparison between genomic DNA and coding sequences revealed that both ZmDGAT1a and ZmDGAT1b comprised 16 exons and 15 introns with a coding region of 1485 and 1488 bp, respectively. ZmDGAT1a and ZmDGAT1b shared similar molecular mass, isoelectric point, and transmembrane domains. ZmDGAT1b was expressed in all sampled tissues that were examined by using reverse transcription-polymerase chain reaction (RT-PCR). In contrast, ZmDGAT1a expression was limited to the silks, tassels, and seeds of different ages. This suggests that the two genes do not have a complementary relationship. The ZmDGAT1a and ZmDGAT1b proteins were subsequently found to be localized in the endoplasmic reticulum (ER). The data provides an important basis for further studies of the functions of DGAT1 genes and for genetic modification of the oil content and composition of corn seeds. |
topic |
maize diacylglycerol acyltransferase expression pattern evolution gene |
url |
http://dx.doi.org/10.1080/13102818.2016.1157036 |
work_keys_str_mv |
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