The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis
There is increasing evidence that epoxiconazole exposure can affect reproductive function, but few studies have investigated adverse effects on spermatogenesis. The nematode Caenorhabditis elegans (C. elegans) was used in our study to assess effects of epoxiconazole on spermatogenesis in male nemato...
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doaj-de2e438471ac44679de1187f868d231c2020-11-24T22:15:04ZengMDPI AGInternational Journal of Environmental Research and Public Health1660-46012016-10-01131099310.3390/ijerph13100993ijerph13100993The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on SpermatogenesisYunhui Li0Minhui Zhang1Shaojun Li2Rongrong Lv3Pan Chen4Ran Liu5Geyu Liang6Lihong Yin7Key Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, ChinaKey Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, ChinaDepartment of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USAKey Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, ChinaDepartment of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USAKey Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, ChinaKey Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, ChinaKey Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, ChinaThere is increasing evidence that epoxiconazole exposure can affect reproductive function, but few studies have investigated adverse effects on spermatogenesis. The nematode Caenorhabditis elegans (C. elegans) was used in our study to assess effects of epoxiconazole on spermatogenesis in male nematodes after 48 h of exposure to concentrations of 0.1, 1.0, or 10.0 μg/L. The results demonstrated that epoxiconazole exposure affected spermatogenesis, decreasing the number of total germ cells, mitotic cells, meiotic cells and spermatids, spermatid diameter, and cross-sectional area, and inducing mitotic germ cell proliferation arrest, premature entry into meiosis, and sperm activation inhibition; however, sperm transfer showed no abnormal changes. In addition, the results showed that epoxiconazole activated the transforming growth factor-β (TGFβ) signaling pathway and increased the expression levels of gene daf-1, daf-3, daf-4, daf-5 and daf-7 in nematodes. We therefore propose that epoxiconazole acts by activating the TGFβ signaling pathway, leading to the impairment of spermatogenesis and the consequent decline in male fertility.http://www.mdpi.com/1660-4601/13/10/993epoxiconazolespermatogenesisTGF-βCaenorhabditis elegans |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yunhui Li Minhui Zhang Shaojun Li Rongrong Lv Pan Chen Ran Liu Geyu Liang Lihong Yin |
spellingShingle |
Yunhui Li Minhui Zhang Shaojun Li Rongrong Lv Pan Chen Ran Liu Geyu Liang Lihong Yin The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis International Journal of Environmental Research and Public Health epoxiconazole spermatogenesis TGF-β Caenorhabditis elegans |
author_facet |
Yunhui Li Minhui Zhang Shaojun Li Rongrong Lv Pan Chen Ran Liu Geyu Liang Lihong Yin |
author_sort |
Yunhui Li |
title |
The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis |
title_short |
The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis |
title_full |
The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis |
title_fullStr |
The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis |
title_full_unstemmed |
The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis |
title_sort |
use of the nematode caenorhabditis elegans to evaluate the adverse effects of epoxiconazole exposure on spermatogenesis |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1660-4601 |
publishDate |
2016-10-01 |
description |
There is increasing evidence that epoxiconazole exposure can affect reproductive function, but few studies have investigated adverse effects on spermatogenesis. The nematode Caenorhabditis elegans (C. elegans) was used in our study to assess effects of epoxiconazole on spermatogenesis in male nematodes after 48 h of exposure to concentrations of 0.1, 1.0, or 10.0 μg/L. The results demonstrated that epoxiconazole exposure affected spermatogenesis, decreasing the number of total germ cells, mitotic cells, meiotic cells and spermatids, spermatid diameter, and cross-sectional area, and inducing mitotic germ cell proliferation arrest, premature entry into meiosis, and sperm activation inhibition; however, sperm transfer showed no abnormal changes. In addition, the results showed that epoxiconazole activated the transforming growth factor-β (TGFβ) signaling pathway and increased the expression levels of gene daf-1, daf-3, daf-4, daf-5 and daf-7 in nematodes. We therefore propose that epoxiconazole acts by activating the TGFβ signaling pathway, leading to the impairment of spermatogenesis and the consequent decline in male fertility. |
topic |
epoxiconazole spermatogenesis TGF-β Caenorhabditis elegans |
url |
http://www.mdpi.com/1660-4601/13/10/993 |
work_keys_str_mv |
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