Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis
Peptidoglycan (PG), a polymer cross-linked by d-amino acid-containing peptides, is an essential component of the bacterial cell wall. We found that a fluorescent d-alanine analog (FDAA) incorporates chiefly at one of the two poles in Mycobacterium smegmatis but that polar dominance varies as a funct...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2017-09-01
|
Series: | mBio |
Online Access: | http://mbio.asm.org/cgi/content/full/8/5/e01183-17 |
id |
doaj-de4915c0aecf4598b70aa31443a9ba9a |
---|---|
record_format |
Article |
spelling |
doaj-de4915c0aecf4598b70aa31443a9ba9a2021-07-02T07:27:12ZengAmerican Society for MicrobiologymBio2150-75112017-09-0185e01183-1710.1128/mBio.01183-17Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosisHelene BotellaGuangli YangOuathek OuerfelliSabine EhrtCarl F. NathanJulien VaubourgeixChristina L. StallingsPeptidoglycan (PG), a polymer cross-linked by d-amino acid-containing peptides, is an essential component of the bacterial cell wall. We found that a fluorescent d-alanine analog (FDAA) incorporates chiefly at one of the two poles in Mycobacterium smegmatis but that polar dominance varies as a function of the cell cycle in Mycobacterium tuberculosis: immediately after cytokinesis, FDAAs are incorporated chiefly at one of the two poles, but just before cytokinesis, FDAAs are incorporated comparably at both. These observations suggest that mycobacterial PG-synthesizing enzymes are localized in functional compartments at the poles and septum and that the capacity for PG synthesis matures at the new pole in M. tuberculosis. Deeper knowledge of the biology of mycobacterial PG synthesis may help in discovering drugs that disable previously unappreciated steps in the process.http://mbio.asm.org/cgi/content/full/8/5/e01183-17 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Helene Botella Guangli Yang Ouathek Ouerfelli Sabine Ehrt Carl F. Nathan Julien Vaubourgeix Christina L. Stallings |
spellingShingle |
Helene Botella Guangli Yang Ouathek Ouerfelli Sabine Ehrt Carl F. Nathan Julien Vaubourgeix Christina L. Stallings Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis mBio |
author_facet |
Helene Botella Guangli Yang Ouathek Ouerfelli Sabine Ehrt Carl F. Nathan Julien Vaubourgeix Christina L. Stallings |
author_sort |
Helene Botella |
title |
Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis |
title_short |
Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis |
title_full |
Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis |
title_fullStr |
Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis |
title_full_unstemmed |
Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis |
title_sort |
distinct spatiotemporal dynamics of peptidoglycan synthesis between mycobacterium smegmatis and mycobacterium tuberculosis |
publisher |
American Society for Microbiology |
series |
mBio |
issn |
2150-7511 |
publishDate |
2017-09-01 |
description |
Peptidoglycan (PG), a polymer cross-linked by d-amino acid-containing peptides, is an essential component of the bacterial cell wall. We found that a fluorescent d-alanine analog (FDAA) incorporates chiefly at one of the two poles in Mycobacterium smegmatis but that polar dominance varies as a function of the cell cycle in Mycobacterium tuberculosis: immediately after cytokinesis, FDAAs are incorporated chiefly at one of the two poles, but just before cytokinesis, FDAAs are incorporated comparably at both. These observations suggest that mycobacterial PG-synthesizing enzymes are localized in functional compartments at the poles and septum and that the capacity for PG synthesis matures at the new pole in M. tuberculosis. Deeper knowledge of the biology of mycobacterial PG synthesis may help in discovering drugs that disable previously unappreciated steps in the process. |
url |
http://mbio.asm.org/cgi/content/full/8/5/e01183-17 |
work_keys_str_mv |
AT helenebotella distinctspatiotemporaldynamicsofpeptidoglycansynthesisbetweenmycobacteriumsmegmatisandmycobacteriumtuberculosis AT guangliyang distinctspatiotemporaldynamicsofpeptidoglycansynthesisbetweenmycobacteriumsmegmatisandmycobacteriumtuberculosis AT ouathekouerfelli distinctspatiotemporaldynamicsofpeptidoglycansynthesisbetweenmycobacteriumsmegmatisandmycobacteriumtuberculosis AT sabineehrt distinctspatiotemporaldynamicsofpeptidoglycansynthesisbetweenmycobacteriumsmegmatisandmycobacteriumtuberculosis AT carlfnathan distinctspatiotemporaldynamicsofpeptidoglycansynthesisbetweenmycobacteriumsmegmatisandmycobacteriumtuberculosis AT julienvaubourgeix distinctspatiotemporaldynamicsofpeptidoglycansynthesisbetweenmycobacteriumsmegmatisandmycobacteriumtuberculosis AT christinalstallings distinctspatiotemporaldynamicsofpeptidoglycansynthesisbetweenmycobacteriumsmegmatisandmycobacteriumtuberculosis |
_version_ |
1721336078687797248 |