Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE Study
Breastfeeding provides defense against infectious disease during early life. The mechanisms underlying this protection are complex but likely include the vast array of immune cells and components, such as immunoglobulins, in milk. Simply characterizing the concentrations of these bioactives, however...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2021-02-01
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Series: | Frontiers in Immunology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2020.614372/full |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michelle K. McGuire Arlo Z. Randall Antti E. Seppo Kirsi M. Järvinen Courtney L. Meehan Debela Gindola Janet E. Williams Daniel W. Sellen Elizabeth W. Kamau-Mbuthia Egidioh W. Kamundia Samwel Mbugua Sophie E. Moore Sophie E. Moore Andrew M. Prentice James A. Foster Gloria E. Otoo Juan M. Rodríguez Rossina G. Pareja Lars Bode Lars Bode Mark A. McGuire Joseph J. Campo |
spellingShingle |
Michelle K. McGuire Arlo Z. Randall Antti E. Seppo Kirsi M. Järvinen Courtney L. Meehan Debela Gindola Janet E. Williams Daniel W. Sellen Elizabeth W. Kamau-Mbuthia Egidioh W. Kamundia Samwel Mbugua Sophie E. Moore Sophie E. Moore Andrew M. Prentice James A. Foster Gloria E. Otoo Juan M. Rodríguez Rossina G. Pareja Lars Bode Lars Bode Mark A. McGuire Joseph J. Campo Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE Study Frontiers in Immunology human milk immunoglobulins IgA IgG pathogen protein array |
author_facet |
Michelle K. McGuire Arlo Z. Randall Antti E. Seppo Kirsi M. Järvinen Courtney L. Meehan Debela Gindola Janet E. Williams Daniel W. Sellen Elizabeth W. Kamau-Mbuthia Egidioh W. Kamundia Samwel Mbugua Sophie E. Moore Sophie E. Moore Andrew M. Prentice James A. Foster Gloria E. Otoo Juan M. Rodríguez Rossina G. Pareja Lars Bode Lars Bode Mark A. McGuire Joseph J. Campo |
author_sort |
Michelle K. McGuire |
title |
Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE Study |
title_short |
Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE Study |
title_full |
Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE Study |
title_fullStr |
Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE Study |
title_full_unstemmed |
Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE Study |
title_sort |
multipathogen analysis of iga and igg antigen specificity for selected pathogens in milk produced by women from diverse geographical regions: the inspire study |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2021-02-01 |
description |
Breastfeeding provides defense against infectious disease during early life. The mechanisms underlying this protection are complex but likely include the vast array of immune cells and components, such as immunoglobulins, in milk. Simply characterizing the concentrations of these bioactives, however, provides only limited information regarding their potential relationships with disease risk in the recipient infant. Rather, understanding pathogen and antigen specificity profiles of milk-borne immunoglobulins might lead to a more complete understanding of how maternal immunity impacts infant health and wellbeing. Milk produced by women living in 11 geographically dispersed populations was applied to a protein microarray containing antigens from 16 pathogens, including diarrheagenic E. coli, Shigella spp., Salmonella enterica serovar Typhi, Staphylococcus aureus, Streptococcus pneumoniae, Mycobacterium tuberculosis and other pathogens of global health concern, and specific IgA and IgG binding was measured. Our analysis identified novel disease-specific antigen responses and suggests that some IgA and IgG responses vary substantially within and among populations. Patterns of antibody reactivity analyzed by principal component analysis and differential reactivity analysis were associated with either lower-to-middle-income countries (LMICs) or high-income countries (HICs). Antibody levels were generally higher in LMICs than HICs, particularly for Shigella and diarrheagenic E. coli antigens, although sets of S. aureus, S. pneumoniae, and some M. tuberculosis antigens were more reactive in HICs. Differential responses were typically specific to canonical immunodominant antigens, but a set of nondifferential but highly reactive antibodies were specific to antigens possibly universally recognized by antibodies in human milk. This approach provides a promising means to understand how breastfeeding and human milk protect (or do not protect) infants from environmentally relevant pathogens. Furthermore, this approach might lead to interventions to boost population-specific immunity in at-risk breastfeeding mothers and their infants. |
topic |
human milk immunoglobulins IgA IgG pathogen protein array |
url |
https://www.frontiersin.org/articles/10.3389/fimmu.2020.614372/full |
work_keys_str_mv |
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doaj-de55355df58b4dc59f59100a6ff601df2021-02-11T06:34:51ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-02-011110.3389/fimmu.2020.614372614372Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE StudyMichelle K. McGuire0Arlo Z. Randall1Antti E. Seppo2Kirsi M. Järvinen3Courtney L. Meehan4Debela Gindola5Janet E. Williams6Daniel W. Sellen7Elizabeth W. Kamau-Mbuthia8Egidioh W. Kamundia9Samwel Mbugua10Sophie E. Moore11Sophie E. Moore12Andrew M. Prentice13James A. Foster14Gloria E. Otoo15Juan M. Rodríguez16Rossina G. Pareja17Lars Bode18Lars Bode19Mark A. McGuire20Joseph J. Campo21Margaret Ritchie School of Family and Consumer Sciences, University of Idaho, Moscow, ID, United StatesAntigen Discovery Incorporated, Irvine, CA, United StatesDepartment of Pediatrics, University of Rochester, Rochester, NY, United StatesDepartment of Pediatrics, University of Rochester, Rochester, NY, United StatesDepartment of Anthropology, Washington State University, Pullman, WA, United StatesDepartment of Anthropology, Hawassa University, Awasa, EthiopiaDepartment of Animal and Veterinary Science, University of Idaho, Moscow, ID, United StatesDalla Lana School of Public Health, University of Toronto, Toronto, ON, CanadaDepartment of Human Nutrition, Egerton University, Nakuru, KenyaDepartment of Human Nutrition, Egerton University, Nakuru, KenyaDepartment of Human Nutrition, Egerton University, Nakuru, KenyaDepartment of Women and Children’s Health, King’s College London, London, United Kingdom0MRC Unit, The Gambia at the London School of Hygiene and Tropical Medicine, Banjul, Gambia0MRC Unit, The Gambia at the London School of Hygiene and Tropical Medicine, Banjul, Gambia1Department of Biological Sciences, University of Idaho, Moscow, ID, United States2Department of Nutrition and Food Science, University of Ghana, Accra, Ghana3Department of Nutrition and Food Science, Complutense University of Madrid, Madrid, Spain4Nutrition Research Institute, Lima, Peru5Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence, University of California, San Diego, La Jolla, CA, United States6Department of Pediatrics, University of California, San Diego, La Jolla, CA, United StatesDepartment of Animal and Veterinary Science, University of Idaho, Moscow, ID, United StatesAntigen Discovery Incorporated, Irvine, CA, United StatesBreastfeeding provides defense against infectious disease during early life. The mechanisms underlying this protection are complex but likely include the vast array of immune cells and components, such as immunoglobulins, in milk. Simply characterizing the concentrations of these bioactives, however, provides only limited information regarding their potential relationships with disease risk in the recipient infant. Rather, understanding pathogen and antigen specificity profiles of milk-borne immunoglobulins might lead to a more complete understanding of how maternal immunity impacts infant health and wellbeing. Milk produced by women living in 11 geographically dispersed populations was applied to a protein microarray containing antigens from 16 pathogens, including diarrheagenic E. coli, Shigella spp., Salmonella enterica serovar Typhi, Staphylococcus aureus, Streptococcus pneumoniae, Mycobacterium tuberculosis and other pathogens of global health concern, and specific IgA and IgG binding was measured. Our analysis identified novel disease-specific antigen responses and suggests that some IgA and IgG responses vary substantially within and among populations. Patterns of antibody reactivity analyzed by principal component analysis and differential reactivity analysis were associated with either lower-to-middle-income countries (LMICs) or high-income countries (HICs). Antibody levels were generally higher in LMICs than HICs, particularly for Shigella and diarrheagenic E. coli antigens, although sets of S. aureus, S. pneumoniae, and some M. tuberculosis antigens were more reactive in HICs. Differential responses were typically specific to canonical immunodominant antigens, but a set of nondifferential but highly reactive antibodies were specific to antigens possibly universally recognized by antibodies in human milk. This approach provides a promising means to understand how breastfeeding and human milk protect (or do not protect) infants from environmentally relevant pathogens. Furthermore, this approach might lead to interventions to boost population-specific immunity in at-risk breastfeeding mothers and their infants.https://www.frontiersin.org/articles/10.3389/fimmu.2020.614372/fullhuman milkimmunoglobulinsIgAIgGpathogenprotein array |