Comparative verification study of silica gel-coated TLC and HPTLC plates’ performances in separation of opium alkaloids on the basis of their physicochemical properties
Abstract Background Thin layer chromatography (TLC) has been a conventional analytical method which is currently used for separation and detection of drugs in biological and non-biological samples. High-performance thin layer chromatography (HPTLC) has been claimed to have several advantages over TL...
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doaj-de5e0379030f41d2b240ef0f7befc9232020-11-24T21:39:40ZengSpringerOpenJournal of Analytical Science and Technology2093-33712017-11-01811710.1186/s40543-017-0131-zComparative verification study of silica gel-coated TLC and HPTLC plates’ performances in separation of opium alkaloids on the basis of their physicochemical propertiesKimia Esmaiili0Seyed Vahid Shetab-Boushehri1Department of Toxicology and Pharmacology, School of Pharmacy, International Campus, and Razi Drug Research Center, Iran University of Medical SciencesDepartment of Toxicology and Pharmacology, School of Pharmacy, International Campus, and Razi Drug Research Center, Iran University of Medical SciencesAbstract Background Thin layer chromatography (TLC) has been a conventional analytical method which is currently used for separation and detection of drugs in biological and non-biological samples. High-performance thin layer chromatography (HPTLC) has been claimed to have several advantages over TLC due to its smaller adsorbent particle size. Opium is the dried latex obtained from the opium poppy containing several alkaloids. Its precise and accurate detection in biological and non-biological samples is a matter of great importance in forensic and clinical toxicology. Methods In the present work, quality of commercial silica gel-coated TLC and HPTLC plates from the two best-known international manufacturers (Merck and Macherey Nagel) in separation of opium alkaloids were investigated and compared in the fluorescence quenching mode to propose the best plate for separation and identification of opium alkaloids. Particle size, zeta potential, specific surface, density, and scanning electron photomicrographs of silica gels, development time for 10 cm migration distance, number of theoretical plates (N), and resolution (R) were compared between all plates. Results Merck TLC plate showed the best separation of opium alkaloids. Although spherical-particle silica-coated Merck HPTLC plate showed narrower particle size distribution, it did not show better separation than Merck TLC plates. Conclusions On the basis of performance and price of TLC and HPTLC plates and great importance of precise and accurate detection of opium in forensic and clinical toxicology, the present work proposes conventional verified analytical TLC plates (for example Merck-TLC plate) for detection of opium alkaloids in biological and non-biological samples.http://link.springer.com/article/10.1186/s40543-017-0131-zOpium alkaloidsTLCHPTLCPhysicochemical properties |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kimia Esmaiili Seyed Vahid Shetab-Boushehri |
spellingShingle |
Kimia Esmaiili Seyed Vahid Shetab-Boushehri Comparative verification study of silica gel-coated TLC and HPTLC plates’ performances in separation of opium alkaloids on the basis of their physicochemical properties Journal of Analytical Science and Technology Opium alkaloids TLC HPTLC Physicochemical properties |
author_facet |
Kimia Esmaiili Seyed Vahid Shetab-Boushehri |
author_sort |
Kimia Esmaiili |
title |
Comparative verification study of silica gel-coated TLC and HPTLC plates’ performances in separation of opium alkaloids on the basis of their physicochemical properties |
title_short |
Comparative verification study of silica gel-coated TLC and HPTLC plates’ performances in separation of opium alkaloids on the basis of their physicochemical properties |
title_full |
Comparative verification study of silica gel-coated TLC and HPTLC plates’ performances in separation of opium alkaloids on the basis of their physicochemical properties |
title_fullStr |
Comparative verification study of silica gel-coated TLC and HPTLC plates’ performances in separation of opium alkaloids on the basis of their physicochemical properties |
title_full_unstemmed |
Comparative verification study of silica gel-coated TLC and HPTLC plates’ performances in separation of opium alkaloids on the basis of their physicochemical properties |
title_sort |
comparative verification study of silica gel-coated tlc and hptlc plates’ performances in separation of opium alkaloids on the basis of their physicochemical properties |
publisher |
SpringerOpen |
series |
Journal of Analytical Science and Technology |
issn |
2093-3371 |
publishDate |
2017-11-01 |
description |
Abstract Background Thin layer chromatography (TLC) has been a conventional analytical method which is currently used for separation and detection of drugs in biological and non-biological samples. High-performance thin layer chromatography (HPTLC) has been claimed to have several advantages over TLC due to its smaller adsorbent particle size. Opium is the dried latex obtained from the opium poppy containing several alkaloids. Its precise and accurate detection in biological and non-biological samples is a matter of great importance in forensic and clinical toxicology. Methods In the present work, quality of commercial silica gel-coated TLC and HPTLC plates from the two best-known international manufacturers (Merck and Macherey Nagel) in separation of opium alkaloids were investigated and compared in the fluorescence quenching mode to propose the best plate for separation and identification of opium alkaloids. Particle size, zeta potential, specific surface, density, and scanning electron photomicrographs of silica gels, development time for 10 cm migration distance, number of theoretical plates (N), and resolution (R) were compared between all plates. Results Merck TLC plate showed the best separation of opium alkaloids. Although spherical-particle silica-coated Merck HPTLC plate showed narrower particle size distribution, it did not show better separation than Merck TLC plates. Conclusions On the basis of performance and price of TLC and HPTLC plates and great importance of precise and accurate detection of opium in forensic and clinical toxicology, the present work proposes conventional verified analytical TLC plates (for example Merck-TLC plate) for detection of opium alkaloids in biological and non-biological samples. |
topic |
Opium alkaloids TLC HPTLC Physicochemical properties |
url |
http://link.springer.com/article/10.1186/s40543-017-0131-z |
work_keys_str_mv |
AT kimiaesmaiili comparativeverificationstudyofsilicagelcoatedtlcandhptlcplatesperformancesinseparationofopiumalkaloidsonthebasisoftheirphysicochemicalproperties AT seyedvahidshetabboushehri comparativeverificationstudyofsilicagelcoatedtlcandhptlcplatesperformancesinseparationofopiumalkaloidsonthebasisoftheirphysicochemicalproperties |
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1725929921236172800 |