3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study
Type 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medic...
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doaj-de7a79078c7d43fdbd727c974be8f8d92020-11-25T00:15:19ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/4326454326453D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo StudyDi Wu0Jian Wan1Yan Huang2Yibing Guo3Tianxin Xu4Mingyan Zhu5Xiangjun Fan6Shajun Zhu7Changchun Ling8Xiaohong Li9Jingjing Lu10Hui Zhu11Pengcheng Zhou12Yuhua Lu13Zhiwei Wang14Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaSurgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaSurgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaSurgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaSurgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaSurgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaType 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medicine methods such as a tissue engineered pancreas seem to provide a useful method. In order to construct a microenvironment similar to the native pancreas that is suitable for not only cell growth but also cellular function exertion, a decellularized mouse pancreas was used as a natural 3D scaffold in this experiment. MIN-6 β cells were planted in the bioscaffold. The cell engraftment was verified by HE staining and SEM. Immunostaining procedures were performed to confirm the normal function of the engrafted cells. qRT-PCR demonstrated that insulin gene expression of the recellularized pancreas was upregulated compared with conventional plate-cultured cells. In vivo experiment was also accomplished to further evaluate the function of the recellularized bioscaffold and the result was inspiring. And beyond doubt this will bring new hope for type 1 diabetic patients.http://dx.doi.org/10.1155/2015/432645 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Di Wu Jian Wan Yan Huang Yibing Guo Tianxin Xu Mingyan Zhu Xiangjun Fan Shajun Zhu Changchun Ling Xiaohong Li Jingjing Lu Hui Zhu Pengcheng Zhou Yuhua Lu Zhiwei Wang |
spellingShingle |
Di Wu Jian Wan Yan Huang Yibing Guo Tianxin Xu Mingyan Zhu Xiangjun Fan Shajun Zhu Changchun Ling Xiaohong Li Jingjing Lu Hui Zhu Pengcheng Zhou Yuhua Lu Zhiwei Wang 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study BioMed Research International |
author_facet |
Di Wu Jian Wan Yan Huang Yibing Guo Tianxin Xu Mingyan Zhu Xiangjun Fan Shajun Zhu Changchun Ling Xiaohong Li Jingjing Lu Hui Zhu Pengcheng Zhou Yuhua Lu Zhiwei Wang |
author_sort |
Di Wu |
title |
3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title_short |
3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title_full |
3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title_fullStr |
3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title_full_unstemmed |
3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title_sort |
3d culture of min-6 cells on decellularized pancreatic scaffold: in vitro and in vivo study |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Type 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medicine methods such as a tissue engineered pancreas seem to provide a useful method. In order to construct a microenvironment similar to the native pancreas that is suitable for not only cell growth but also cellular function exertion, a decellularized mouse pancreas was used as a natural 3D scaffold in this experiment. MIN-6 β cells were planted in the bioscaffold. The cell engraftment was verified by HE staining and SEM. Immunostaining procedures were performed to confirm the normal function of the engrafted cells. qRT-PCR demonstrated that insulin gene expression of the recellularized pancreas was upregulated compared with conventional plate-cultured cells. In vivo experiment was also accomplished to further evaluate the function of the recellularized bioscaffold and the result was inspiring. And beyond doubt this will bring new hope for type 1 diabetic patients. |
url |
http://dx.doi.org/10.1155/2015/432645 |
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