Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™

Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™

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Method of identification ofT- and B-lymphocytes in chickens and their subpopulations by flow cytofluorometric analysis using BD FACSVerseTM was developed. Method of lymphocytic gate extraction with control of leukocyte common antigen CD45 and monocytic antigen expression was determined. Antibody pan...

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Main Authors: M. A. Volkova, Ir. A. Chvala
Format: Article
Language:English
Published: Da Vinci Media 2018-04-01
Series:Ветеринария сегодня
Subjects:
flow cytofluorometric analysis
immunophenotyping of chicken lymphocytes
Online Access:https://veterinary.arriah.ru/jour/article/view/194
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spelling doaj-de83649460cd415ab800a4f7af1c71ab2021-07-30T06:29:22ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-01024347194Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™M. A. Volkova0Ir. A. Chvala1FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirMethod of identification ofT- and B-lymphocytes in chickens and their subpopulations by flow cytofluorometric analysis using BD FACSVerseTM was developed. Method of lymphocytic gate extraction with control of leukocyte common antigen CD45 and monocytic antigen expression was determined. Antibody panels for immunophenotyping lymphocytes when testing chicken cell mediated immune response were selected and tested. They enable to determine a relative number of T- and B-lymphocytes, T-helpers and T-cytotoxic cell populations in blood and chicken lymphoid organs. The paper demonstrates specificity and reproducibility of the developed method of identification of chicken lymphocyte subpopulation.https://veterinary.arriah.ru/jour/article/view/194flow cytofluorometric analysisimmunophenotyping of chicken lymphocytes
collection DOAJ
language English
format Article
sources DOAJ
author M. A. Volkova
Ir. A. Chvala
spellingShingle M. A. Volkova
Ir. A. Chvala
Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™
Ветеринария сегодня
flow cytofluorometric analysis
immunophenotyping of chicken lymphocytes
author_facet M. A. Volkova
Ir. A. Chvala
author_sort M. A. Volkova
title Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™
title_short Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™
title_full Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™
title_fullStr Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™
title_full_unstemmed Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™
title_sort identification of t and в lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer bd facsverse™
publisher Da Vinci Media
series Ветеринария сегодня
issn 2304-196X
2658-6959
publishDate 2018-04-01
description Method of identification ofT- and B-lymphocytes in chickens and their subpopulations by flow cytofluorometric analysis using BD FACSVerseTM was developed. Method of lymphocytic gate extraction with control of leukocyte common antigen CD45 and monocytic antigen expression was determined. Antibody panels for immunophenotyping lymphocytes when testing chicken cell mediated immune response were selected and tested. They enable to determine a relative number of T- and B-lymphocytes, T-helpers and T-cytotoxic cell populations in blood and chicken lymphoid organs. The paper demonstrates specificity and reproducibility of the developed method of identification of chicken lymphocyte subpopulation.
topic flow cytofluorometric analysis
immunophenotyping of chicken lymphocytes
url https://veterinary.arriah.ru/jour/article/view/194
work_keys_str_mv AT mavolkova identificationoftandvlymphocytesinchickensandtheirsubpopulationsbyflowcytofluorometricanalysisusingflowcytometerbdfacsverse
AT irachvala identificationoftandvlymphocytesinchickensandtheirsubpopulationsbyflowcytofluorometricanalysisusingflowcytometerbdfacsverse
_version_ 1714390422291218432

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