Nucleic Acid Testing of SARS-CoV-2
The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. The polymerase chain reaction (PCR)-based method including reverse transcription-polymerase chain reaction (RT-PCR) is the most widely used assay...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-06-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/22/11/6150 |
id |
doaj-de8fd891c591482091c4498a7826f130 |
---|---|
record_format |
Article |
spelling |
doaj-de8fd891c591482091c4498a7826f1302021-06-30T23:31:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-01226150615010.3390/ijms22116150Nucleic Acid Testing of SARS-CoV-2Hee Min Yoo0Il-Hwan Kim1Seil Kim2Microbiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, KoreaMicrobiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, KoreaMicrobiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, KoreaThe coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. The polymerase chain reaction (PCR)-based method including reverse transcription-polymerase chain reaction (RT-PCR) is the most widely used assay for the detection of SARS-CoV-2 RNA. Along with the RT-PCR method, digital PCR has emerged as a powerful tool to quantify nucleic acid of the virus with high accuracy and sensitivity. Non-PCR based techniques such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription recombinase polymerase amplification (RT-RPA) are considered to be rapid and simple nucleic acid detection methods and were reviewed in this paper. Non-conventional molecular diagnostic methods including next-generation sequencing (NGS), CRISPR-based assays and nanotechnology are improving the accuracy and sensitivity of COVID-19 diagnosis. In this review, we also focus on standardization of SARS-CoV-2 nucleic acid testing and the activity of the National Metrology Institutes (NMIs) and highlight resources such as reference materials (RM) that provide the values of specified properties. Finally, we summarize the useful resources for convenient COVID-19 molecular diagnostics.https://www.mdpi.com/1422-0067/22/11/6150SARS-CoV-2PCRisothermal amplificationgenome sequencingnucleic acid testingreference materials |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hee Min Yoo Il-Hwan Kim Seil Kim |
spellingShingle |
Hee Min Yoo Il-Hwan Kim Seil Kim Nucleic Acid Testing of SARS-CoV-2 International Journal of Molecular Sciences SARS-CoV-2 PCR isothermal amplification genome sequencing nucleic acid testing reference materials |
author_facet |
Hee Min Yoo Il-Hwan Kim Seil Kim |
author_sort |
Hee Min Yoo |
title |
Nucleic Acid Testing of SARS-CoV-2 |
title_short |
Nucleic Acid Testing of SARS-CoV-2 |
title_full |
Nucleic Acid Testing of SARS-CoV-2 |
title_fullStr |
Nucleic Acid Testing of SARS-CoV-2 |
title_full_unstemmed |
Nucleic Acid Testing of SARS-CoV-2 |
title_sort |
nucleic acid testing of sars-cov-2 |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-06-01 |
description |
The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. The polymerase chain reaction (PCR)-based method including reverse transcription-polymerase chain reaction (RT-PCR) is the most widely used assay for the detection of SARS-CoV-2 RNA. Along with the RT-PCR method, digital PCR has emerged as a powerful tool to quantify nucleic acid of the virus with high accuracy and sensitivity. Non-PCR based techniques such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription recombinase polymerase amplification (RT-RPA) are considered to be rapid and simple nucleic acid detection methods and were reviewed in this paper. Non-conventional molecular diagnostic methods including next-generation sequencing (NGS), CRISPR-based assays and nanotechnology are improving the accuracy and sensitivity of COVID-19 diagnosis. In this review, we also focus on standardization of SARS-CoV-2 nucleic acid testing and the activity of the National Metrology Institutes (NMIs) and highlight resources such as reference materials (RM) that provide the values of specified properties. Finally, we summarize the useful resources for convenient COVID-19 molecular diagnostics. |
topic |
SARS-CoV-2 PCR isothermal amplification genome sequencing nucleic acid testing reference materials |
url |
https://www.mdpi.com/1422-0067/22/11/6150 |
work_keys_str_mv |
AT heeminyoo nucleicacidtestingofsarscov2 AT ilhwankim nucleicacidtestingofsarscov2 AT seilkim nucleicacidtestingofsarscov2 |
_version_ |
1721351162418954240 |