Mapping-by-sequencing using NGS-based 3′-MACE-Seq reveals a new mutant allele of the essential nodulation gene Sym33 (IPD3) in pea (Pisum sativum L.)

Mapping-by-sequencing using NGS-based 3′-MACE-Seq reveals a new mutant allele of the essential nodulation gene Sym33 (IPD3) in pea (Pisum sativum L.)

Large collections of pea symbiotic mutants were accumulated in the 1990s, but the causal genes for a large portion of the mutations are still not identified due to the complexity of the task. We applied a Mapping-by-Sequencing approach including Bulk Segregant Analysis and Massive Analysis of cDNA E...

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Bibliographic Details
Main Authors: Aleksandr I. Zhernakov, Oksana Y. Shtark, Olga A. Kulaeva, Jaroslava V. Fedorina, Alexey M. Afonin, Anna B. Kitaeva, Viktor E. Tsyganov, Fabian Afonso-Grunz, Klaus Hoffmeier, Björn Rotter, Peter Winter, Igor A. Tikhonovich, Vladimir A. Zhukov
Format: Article
Language:English
Published: PeerJ Inc. 2019-04-01
Series:PeerJ
Subjects:
Mapping-by-sequencing
Next generation sequencing
RNA-Seq
Massive analysis of cDNA Ends
Pea
Symbiotic genes
Online Access:https://peerj.com/articles/6662.pdf

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