Force measurements of TCR/pMHC recognition at T cell surface.
The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends on the nature of the pep...
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2011-01-01
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doaj-deb8d1e6c388403ebda5c97934e266522020-11-25T01:35:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2234410.1371/journal.pone.0022344Force measurements of TCR/pMHC recognition at T cell surface.Pierre-Henri PuechDamien NevoltrisPhilippe RobertLaurent LimozinClaude BoyerPierre BongrandThe rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends on the nature of the peptide but the rupture force does not. When CD8 is present, no effect of the nature of the peptide is observed. CD8 is proposed to act as a time and distance lock, enabling the shorter TCR molecule to bridge the pMHC and have time to finely read the peptide. Ultimately, such experiments could help the dissection of the sequential steps by which the TCR reads the peptide/MHC complex in order to control T cell activation.http://europepmc.org/articles/PMC3142151?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pierre-Henri Puech Damien Nevoltris Philippe Robert Laurent Limozin Claude Boyer Pierre Bongrand |
spellingShingle |
Pierre-Henri Puech Damien Nevoltris Philippe Robert Laurent Limozin Claude Boyer Pierre Bongrand Force measurements of TCR/pMHC recognition at T cell surface. PLoS ONE |
author_facet |
Pierre-Henri Puech Damien Nevoltris Philippe Robert Laurent Limozin Claude Boyer Pierre Bongrand |
author_sort |
Pierre-Henri Puech |
title |
Force measurements of TCR/pMHC recognition at T cell surface. |
title_short |
Force measurements of TCR/pMHC recognition at T cell surface. |
title_full |
Force measurements of TCR/pMHC recognition at T cell surface. |
title_fullStr |
Force measurements of TCR/pMHC recognition at T cell surface. |
title_full_unstemmed |
Force measurements of TCR/pMHC recognition at T cell surface. |
title_sort |
force measurements of tcr/pmhc recognition at t cell surface. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends on the nature of the peptide but the rupture force does not. When CD8 is present, no effect of the nature of the peptide is observed. CD8 is proposed to act as a time and distance lock, enabling the shorter TCR molecule to bridge the pMHC and have time to finely read the peptide. Ultimately, such experiments could help the dissection of the sequential steps by which the TCR reads the peptide/MHC complex in order to control T cell activation. |
url |
http://europepmc.org/articles/PMC3142151?pdf=render |
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