Semen bacterial contamination: Antibiotics susceptibility pattern and standard sperm parameters in men of Birjand, 2017

Background and Aim: Urinary tract infections can be one of the most important causes of infertility in men. Identification of semen bacterial contamination and using of appropriate antibiotic treatment can cause to improvement sperm parameters. The aim of this study was to evaluate the frequency of...

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Main Authors: Mohamad Reza Doostabadi, Diba Abazari, Masoud Yousefi, Ali Hosseininejad-Mohebati, Sedigheh Solymani, Mohsen Foadoddini
Format: Article
Language:fas
Published: Birjand University of Medical Sciences and Health Services 2020-06-01
Series:Journal of Birjand University of Medical Sciences
Subjects:
Online Access:http://journal.bums.ac.ir/article-1-2740-en.html
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spelling doaj-dec3cecabc684fb38fc66cf366ccfad52020-11-25T02:36:55ZfasBirjand University of Medical Sciences and Health ServicesJournal of Birjand University of Medical Sciences1607-21972423-61522020-06-01272172181Semen bacterial contamination: Antibiotics susceptibility pattern and standard sperm parameters in men of Birjand, 2017Mohamad Reza Doostabadi0Diba Abazari1Masoud Yousefi2Ali Hosseininejad-Mohebati3Sedigheh Solymani4Mohsen Foadoddini5 Yazd Reproductive Sciences Institute, Yazd University of Medical Science, Yazd, Iran Medical Doctor (M.D), Birjand University of Medical Sciences, Birjand, Iran. Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran. Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran. Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran. Background and Aim: Urinary tract infections can be one of the most important causes of infertility in men. Identification of semen bacterial contamination and using of appropriate antibiotic treatment can cause to improvement sperm parameters. The aim of this study was to evaluate the frequency of bacterial contamination of semen and its effect on standard sperm parameters. Materials and Methods: In this descriptive-analytical study, the semen analysis of 40 men referring to Birjand laboratories was performed by using the CASA model spermogram device. The Eosin-Nigrosin staining was used to evaluate of sperm viability. After semen samples were cultured on microbial culture media, bacterial contamination was assessed by using conventional microbiological methods. The determination of antibiotic susceptibility of bacterial isolates was performed by the disk diffusion method. Results: In the present study, 30% of semen 12 samples were detected with bacterial contamination, the most frequent of which was related to Escherichia coli (41.7%). Statistical analysis showed that there was a significant relationship between bacterial contamination of semen and history of urinary tract infection (UTI) (P=0.001). There was a significant decrease in the motility, viability and sperm count in subjects with bacterial semen contamination (P<0.05). Nitrofurantoin, Gentamicin and third-generation of Cephalosporins were reported as the most effective treatment options for reducing bacterial contamination in semen. Conclusion: The bacterial contamination of semen can significantly reduce the motility, viability and sperm count. Due to the high prevalence of bacterial contamination in semen and its significant association with sperm factors, the microbial screening of infertile couples without clinical symptoms is essential.http://journal.bums.ac.ir/article-1-2740-en.htmlantibiotic resistancebacterial contaminationinfertilitysemen fluidsperm
collection DOAJ
language fas
format Article
sources DOAJ
author Mohamad Reza Doostabadi
Diba Abazari
Masoud Yousefi
Ali Hosseininejad-Mohebati
Sedigheh Solymani
Mohsen Foadoddini
spellingShingle Mohamad Reza Doostabadi
Diba Abazari
Masoud Yousefi
Ali Hosseininejad-Mohebati
Sedigheh Solymani
Mohsen Foadoddini
Semen bacterial contamination: Antibiotics susceptibility pattern and standard sperm parameters in men of Birjand, 2017
Journal of Birjand University of Medical Sciences
antibiotic resistance
bacterial contamination
infertility
semen fluid
sperm
author_facet Mohamad Reza Doostabadi
Diba Abazari
Masoud Yousefi
Ali Hosseininejad-Mohebati
Sedigheh Solymani
Mohsen Foadoddini
author_sort Mohamad Reza Doostabadi
title Semen bacterial contamination: Antibiotics susceptibility pattern and standard sperm parameters in men of Birjand, 2017
title_short Semen bacterial contamination: Antibiotics susceptibility pattern and standard sperm parameters in men of Birjand, 2017
title_full Semen bacterial contamination: Antibiotics susceptibility pattern and standard sperm parameters in men of Birjand, 2017
title_fullStr Semen bacterial contamination: Antibiotics susceptibility pattern and standard sperm parameters in men of Birjand, 2017
title_full_unstemmed Semen bacterial contamination: Antibiotics susceptibility pattern and standard sperm parameters in men of Birjand, 2017
title_sort semen bacterial contamination: antibiotics susceptibility pattern and standard sperm parameters in men of birjand, 2017
publisher Birjand University of Medical Sciences and Health Services
series Journal of Birjand University of Medical Sciences
issn 1607-2197
2423-6152
publishDate 2020-06-01
description Background and Aim: Urinary tract infections can be one of the most important causes of infertility in men. Identification of semen bacterial contamination and using of appropriate antibiotic treatment can cause to improvement sperm parameters. The aim of this study was to evaluate the frequency of bacterial contamination of semen and its effect on standard sperm parameters. Materials and Methods: In this descriptive-analytical study, the semen analysis of 40 men referring to Birjand laboratories was performed by using the CASA model spermogram device. The Eosin-Nigrosin staining was used to evaluate of sperm viability. After semen samples were cultured on microbial culture media, bacterial contamination was assessed by using conventional microbiological methods. The determination of antibiotic susceptibility of bacterial isolates was performed by the disk diffusion method. Results: In the present study, 30% of semen 12 samples were detected with bacterial contamination, the most frequent of which was related to Escherichia coli (41.7%). Statistical analysis showed that there was a significant relationship between bacterial contamination of semen and history of urinary tract infection (UTI) (P=0.001). There was a significant decrease in the motility, viability and sperm count in subjects with bacterial semen contamination (P<0.05). Nitrofurantoin, Gentamicin and third-generation of Cephalosporins were reported as the most effective treatment options for reducing bacterial contamination in semen. Conclusion: The bacterial contamination of semen can significantly reduce the motility, viability and sperm count. Due to the high prevalence of bacterial contamination in semen and its significant association with sperm factors, the microbial screening of infertile couples without clinical symptoms is essential.
topic antibiotic resistance
bacterial contamination
infertility
semen fluid
sperm
url http://journal.bums.ac.ir/article-1-2740-en.html
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