Transcriptomic Analysis of Spleen Revealed Mechanism of Dexamethasone-Induced Immune Suppression in Chicks
Stress-induced immunosuppression is a common problem in the poultry industry, but the specific mechanism of its effect on the immune function of chicken has not been clarified. In this study, 7-day-old Gushi cocks were selected as subjects, and a stress-induced immunosuppression model was successful...
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doaj-dec6f2c827294503a9aef35b77d186262020-11-25T03:13:30ZengMDPI AGGenes2073-44252020-05-011151351310.3390/genes11050513Transcriptomic Analysis of Spleen Revealed Mechanism of Dexamethasone-Induced Immune Suppression in ChicksYujie Guo0Aru Su1Huihui Tian2Minxi Zhai3Wenting Li4Yadong Tian5Kui Li6Guirong Sun7Ruirui Jiang8Ruili Han9Fengbin Yan10Xiangtao Kang11College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, ChinaStress-induced immunosuppression is a common problem in the poultry industry, but the specific mechanism of its effect on the immune function of chicken has not been clarified. In this study, 7-day-old Gushi cocks were selected as subjects, and a stress-induced immunosuppression model was successfully established via daily injection of 2.0 mg/kg (body weight) dexamethasone. We characterized the spleen transcriptome in the control (B_S) and model (D_S) groups, and 515 significant differentially expressed genes (SDEGs) (Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced (FPKM) > 1, adjusted <i>p</i>-value (padj) < 0.05 and Fold change (|FC|) ≥ 2) were identified. The cytokine-cytokine receptor interaction signaling pathway was identified as being highly activated during stress-induced immunosuppression, including the following SDEGs—<i>CXCL13L2, CSF3R, CSF2RB, CCR9, CCR10, IL1R1, IL8L1, IL8L2, GHR, KIT, OSMR, TNFRSF13B, TNFSF13B</i>, and <i>TGFBR2L</i>. At the same time, immune-related SDEGs including <i>CCR9</i>, <i>CCR10, DMB1, TNFRSF13B, TNFRSF13C </i>and<i> TNFSF13B</i> were significantly enriched in the intestinal immune network for the IgA production signaling pathway. The SDEG protein-protein interaction module analysis showed that <i>CXCR5, CCR8L, CCR9, CCR10, IL8L2, IL8L1, TNFSF13B, TNFRSF13B</i> and <i>TNFRSF13C</i> may play an important role in stress-induced immunosuppression. These findings provide a background for further research on stress-induced immunosuppression. Thus, we can better understand the molecular genetic mechanism of chicken stress-induced immunosuppression.https://www.mdpi.com/2073-4425/11/5/513chickenspleenstressimmunosuppressionRNA-seq |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yujie Guo Aru Su Huihui Tian Minxi Zhai Wenting Li Yadong Tian Kui Li Guirong Sun Ruirui Jiang Ruili Han Fengbin Yan Xiangtao Kang |
spellingShingle |
Yujie Guo Aru Su Huihui Tian Minxi Zhai Wenting Li Yadong Tian Kui Li Guirong Sun Ruirui Jiang Ruili Han Fengbin Yan Xiangtao Kang Transcriptomic Analysis of Spleen Revealed Mechanism of Dexamethasone-Induced Immune Suppression in Chicks Genes chicken spleen stress immunosuppression RNA-seq |
author_facet |
Yujie Guo Aru Su Huihui Tian Minxi Zhai Wenting Li Yadong Tian Kui Li Guirong Sun Ruirui Jiang Ruili Han Fengbin Yan Xiangtao Kang |
author_sort |
Yujie Guo |
title |
Transcriptomic Analysis of Spleen Revealed Mechanism of Dexamethasone-Induced Immune Suppression in Chicks |
title_short |
Transcriptomic Analysis of Spleen Revealed Mechanism of Dexamethasone-Induced Immune Suppression in Chicks |
title_full |
Transcriptomic Analysis of Spleen Revealed Mechanism of Dexamethasone-Induced Immune Suppression in Chicks |
title_fullStr |
Transcriptomic Analysis of Spleen Revealed Mechanism of Dexamethasone-Induced Immune Suppression in Chicks |
title_full_unstemmed |
Transcriptomic Analysis of Spleen Revealed Mechanism of Dexamethasone-Induced Immune Suppression in Chicks |
title_sort |
transcriptomic analysis of spleen revealed mechanism of dexamethasone-induced immune suppression in chicks |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2020-05-01 |
description |
Stress-induced immunosuppression is a common problem in the poultry industry, but the specific mechanism of its effect on the immune function of chicken has not been clarified. In this study, 7-day-old Gushi cocks were selected as subjects, and a stress-induced immunosuppression model was successfully established via daily injection of 2.0 mg/kg (body weight) dexamethasone. We characterized the spleen transcriptome in the control (B_S) and model (D_S) groups, and 515 significant differentially expressed genes (SDEGs) (Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced (FPKM) > 1, adjusted <i>p</i>-value (padj) < 0.05 and Fold change (|FC|) ≥ 2) were identified. The cytokine-cytokine receptor interaction signaling pathway was identified as being highly activated during stress-induced immunosuppression, including the following SDEGs—<i>CXCL13L2, CSF3R, CSF2RB, CCR9, CCR10, IL1R1, IL8L1, IL8L2, GHR, KIT, OSMR, TNFRSF13B, TNFSF13B</i>, and <i>TGFBR2L</i>. At the same time, immune-related SDEGs including <i>CCR9</i>, <i>CCR10, DMB1, TNFRSF13B, TNFRSF13C </i>and<i> TNFSF13B</i> were significantly enriched in the intestinal immune network for the IgA production signaling pathway. The SDEG protein-protein interaction module analysis showed that <i>CXCR5, CCR8L, CCR9, CCR10, IL8L2, IL8L1, TNFSF13B, TNFRSF13B</i> and <i>TNFRSF13C</i> may play an important role in stress-induced immunosuppression. These findings provide a background for further research on stress-induced immunosuppression. Thus, we can better understand the molecular genetic mechanism of chicken stress-induced immunosuppression. |
topic |
chicken spleen stress immunosuppression RNA-seq |
url |
https://www.mdpi.com/2073-4425/11/5/513 |
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