Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated Goats
Current eradication strategies of tuberculosis (TB) in goats mainly rely on the single intradermal tuberculin test (SIT) and single intradermal cervical comparative tuberculin tests (SICCTs). TB vaccination has been proposed as a cost-effective option in high-prevalence herds or countries where econ...
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doaj-ded050bd201e4f37a2389a2dc000c01e2020-11-25T03:34:23ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-07-01710.3389/fvets.2020.00374540953Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated GoatsClaudia Arrieta-Villegas0José Antonio Infantes-Lorenzo1Javier Bezos2Javier Bezos3Miriam Grasa4Enric Vidal5Irene Mercader6Mahavir Singh7Mariano Domingo8Mariano Domingo9Lucía de Juan10Lucía de Juan11Bernat Pérez de Val12IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus Universitat Autònoma de Barcelona, Barcelona, SpainServicio de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Investigación Carlos III, Madrid, SpainVISAVET Health Surveillance Center, Universidad Complutense de Madrid, Madrid, SpainDepartamento de Sanidad Animal, Universidad Complutense de Madrid, Madrid, SpainAgrupació de Defensa Sanitària de Cabrum i Oví Lleter de Catalunya, Barbens, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus Universitat Autònoma de Barcelona, Barcelona, SpainDepartament d'Agricultura, Ramaderia, Pesca i Alimentació de la Generalitat de Catalunya, Barcelona, SpainLionex Diagnostics and Therapeutics GmbH, Braunschweig, GermanyIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus Universitat Autònoma de Barcelona, Barcelona, SpainDepartament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona (UAB), Barcelona, SpainVISAVET Health Surveillance Center, Universidad Complutense de Madrid, Madrid, SpainDepartamento de Sanidad Animal, Universidad Complutense de Madrid, Madrid, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus Universitat Autònoma de Barcelona, Barcelona, SpainCurrent eradication strategies of tuberculosis (TB) in goats mainly rely on the single intradermal tuberculin test (SIT) and single intradermal cervical comparative tuberculin tests (SICCTs). TB vaccination has been proposed as a cost-effective option in high-prevalence herds or countries where economic compensation for the slaughter of positive animals is not affordable. However, TB vaccination compromises the efficiency of tuberculin-based diagnostic tests. In this study, the performance of a new diagnostic platform, based on the P22 antigenic complex, was assessed for skin test (ST), interferon-gamma release assay (IGRA), and serology under different TB scenarios. The sensitivity (Se) of diagnostic tests was assessed in TB-infected goats from the same farm (herd A, N = 77). The specificity (Sp) was assessed in two TB-negative farms (both vaccinated against paratuberculosis): one TB unvaccinated (herd B, N = 77) and another vaccinated with bacille Calmette-Guérin (BCG) (herd C, N = 68). The single (s) P22-IGRA showed the highest Se among IGRA tests (91%), and the comparative (c) P22-ST showed the highest Sp (100% in herd B and 98% in herd C). Combined interpretation of techniques enabled the best diagnostic performances. Combining the SICCT + sP22-IGRA improved Se (97%) compared to SICCT + tuberculin-based IGRA (95%), with a reduction of Sp (95 and 100%, respectively). Besides, combination of P22-ELISA with cP22-ST or SICCT elicited a similar performance in the non-vaccination context (Se: 94 and 95%; Sp: 95 and 95%, respectively), but Sp was significantly higher for the combination with cP22-ST compared to SICCT in the TB vaccination context (95 and 79%, respectively). The combination of serological tests based on P22 and MPB83 showed higher complementarity and improved 13 percentage points the Se of P22-ELISA alone. These findings suggest that either cell-mediated or antibody-based diagnostic techniques, using the P22 antigen complex, can contribute to improve the immunodiagnostics of TB in goats under different TB control strategies.https://www.frontiersin.org/article/10.3389/fvets.2020.00374/fulltuberculosisdiagnosisgoatsbacille Calmette-Guérin (BCG)skin testinterferon-gamma release assay (IGRA) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Claudia Arrieta-Villegas José Antonio Infantes-Lorenzo Javier Bezos Javier Bezos Miriam Grasa Enric Vidal Irene Mercader Mahavir Singh Mariano Domingo Mariano Domingo Lucía de Juan Lucía de Juan Bernat Pérez de Val |
spellingShingle |
Claudia Arrieta-Villegas José Antonio Infantes-Lorenzo Javier Bezos Javier Bezos Miriam Grasa Enric Vidal Irene Mercader Mahavir Singh Mariano Domingo Mariano Domingo Lucía de Juan Lucía de Juan Bernat Pérez de Val Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated Goats Frontiers in Veterinary Science tuberculosis diagnosis goats bacille Calmette-Guérin (BCG) skin test interferon-gamma release assay (IGRA) |
author_facet |
Claudia Arrieta-Villegas José Antonio Infantes-Lorenzo Javier Bezos Javier Bezos Miriam Grasa Enric Vidal Irene Mercader Mahavir Singh Mariano Domingo Mariano Domingo Lucía de Juan Lucía de Juan Bernat Pérez de Val |
author_sort |
Claudia Arrieta-Villegas |
title |
Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated Goats |
title_short |
Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated Goats |
title_full |
Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated Goats |
title_fullStr |
Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated Goats |
title_full_unstemmed |
Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated Goats |
title_sort |
evaluation of p22 antigenic complex for the immuno-diagnosis of tuberculosis in bcg vaccinated and unvaccinated goats |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2020-07-01 |
description |
Current eradication strategies of tuberculosis (TB) in goats mainly rely on the single intradermal tuberculin test (SIT) and single intradermal cervical comparative tuberculin tests (SICCTs). TB vaccination has been proposed as a cost-effective option in high-prevalence herds or countries where economic compensation for the slaughter of positive animals is not affordable. However, TB vaccination compromises the efficiency of tuberculin-based diagnostic tests. In this study, the performance of a new diagnostic platform, based on the P22 antigenic complex, was assessed for skin test (ST), interferon-gamma release assay (IGRA), and serology under different TB scenarios. The sensitivity (Se) of diagnostic tests was assessed in TB-infected goats from the same farm (herd A, N = 77). The specificity (Sp) was assessed in two TB-negative farms (both vaccinated against paratuberculosis): one TB unvaccinated (herd B, N = 77) and another vaccinated with bacille Calmette-Guérin (BCG) (herd C, N = 68). The single (s) P22-IGRA showed the highest Se among IGRA tests (91%), and the comparative (c) P22-ST showed the highest Sp (100% in herd B and 98% in herd C). Combined interpretation of techniques enabled the best diagnostic performances. Combining the SICCT + sP22-IGRA improved Se (97%) compared to SICCT + tuberculin-based IGRA (95%), with a reduction of Sp (95 and 100%, respectively). Besides, combination of P22-ELISA with cP22-ST or SICCT elicited a similar performance in the non-vaccination context (Se: 94 and 95%; Sp: 95 and 95%, respectively), but Sp was significantly higher for the combination with cP22-ST compared to SICCT in the TB vaccination context (95 and 79%, respectively). The combination of serological tests based on P22 and MPB83 showed higher complementarity and improved 13 percentage points the Se of P22-ELISA alone. These findings suggest that either cell-mediated or antibody-based diagnostic techniques, using the P22 antigen complex, can contribute to improve the immunodiagnostics of TB in goats under different TB control strategies. |
topic |
tuberculosis diagnosis goats bacille Calmette-Guérin (BCG) skin test interferon-gamma release assay (IGRA) |
url |
https://www.frontiersin.org/article/10.3389/fvets.2020.00374/full |
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