LncRNA LINC00511 Acts as an Oncogene in Colorectal Cancer via Sponging miR-29c-3p to Upregulate NFIA
Yu Hu,1,* Ying Zhang,2,* Meng Ding,2 Ruisi Xu2 1Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, People’s Republic of China; 2Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, People&...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Dove Medical Press
2021-01-01
|
Series: | OncoTargets and Therapy |
Subjects: | |
Online Access: | https://www.dovepress.com/lncrna-linc00511-acts-as-an-oncogene-in-colorectal-cancer-via-sponging-peer-reviewed-article-OTT |
id |
doaj-dee00102dca3489681548ae1e5155807 |
---|---|
record_format |
Article |
spelling |
doaj-dee00102dca3489681548ae1e51558072021-01-06T01:27:12ZengDove Medical PressOncoTargets and Therapy1178-69302021-01-01Volume 13134131342460868LncRNA LINC00511 Acts as an Oncogene in Colorectal Cancer via Sponging miR-29c-3p to Upregulate NFIAHu YZhang YDing MXu RYu Hu,1,* Ying Zhang,2,* Meng Ding,2 Ruisi Xu2 1Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, People’s Republic of China; 2Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, People’s Republic of China*These authors contributed equally to this workCorrespondence: Meng Ding; Ruisi XuChina-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, People’s Republic of ChinaEmail dingmeng@jlu.edu.cn; xuruisi@jlu.edu.cnBackground: Colorectal cancer (CRC), characterized by high mortality and incidence rate, is one of the most common types of rectum tumors in the gastrointestinal tract worldwide. An increasing number of investigations indicated that long noncoding RNAs (lncRNAs) have been implicated in the growth of a wide range of cancers. Although it has obtained general acceptance that lncRNA LINC00511 plays a significant role in numerous cancers, the regulatory mechanism of LINC00511 in CRC still needs to be explored.Materials and Methods: Bioinformatics analysis and a wide range of experiments of sphere formation assay, cell proliferation assay, RT-qPCR, colony formation assay, Transwell assay and Western blot assays investigated the function and mechanism of LINC00511 in CRC tissues and cells.Results: Our results manifested that the expression level of LINC00511 was obviously upregulated in CRC tissues and cells and it accelerated CRC development through facilitating cell proliferation, metastasis and stemness. Molecular mechanism exploration uncovered that LINC00511 acted as a ceRNA competing with NFIA to bind with miR-29c-3p. Through rescue experiments, we discovered that NFIA upregulation partly counteracted the inhibitive effect induced by LINC00511 silencing on CRC progression.Conclusion: These results revealed that LINC00511 participated in the progression of CRC by targeting the LINC00511/miR-29c-3p/NFIA axis, indicating that LINC00511 may be a potential therapeutic target for CRC treatment.Keywords: LINC00511, miR-29c-3p, NFIA, colorectal cancerhttps://www.dovepress.com/lncrna-linc00511-acts-as-an-oncogene-in-colorectal-cancer-via-sponging-peer-reviewed-article-OTTlinc00511mir-29c-3pnfiacolorectal cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hu Y Zhang Y Ding M Xu R |
spellingShingle |
Hu Y Zhang Y Ding M Xu R LncRNA LINC00511 Acts as an Oncogene in Colorectal Cancer via Sponging miR-29c-3p to Upregulate NFIA OncoTargets and Therapy linc00511 mir-29c-3p nfia colorectal cancer |
author_facet |
Hu Y Zhang Y Ding M Xu R |
author_sort |
Hu Y |
title |
LncRNA LINC00511 Acts as an Oncogene in Colorectal Cancer via Sponging miR-29c-3p to Upregulate NFIA |
title_short |
LncRNA LINC00511 Acts as an Oncogene in Colorectal Cancer via Sponging miR-29c-3p to Upregulate NFIA |
title_full |
LncRNA LINC00511 Acts as an Oncogene in Colorectal Cancer via Sponging miR-29c-3p to Upregulate NFIA |
title_fullStr |
LncRNA LINC00511 Acts as an Oncogene in Colorectal Cancer via Sponging miR-29c-3p to Upregulate NFIA |
title_full_unstemmed |
LncRNA LINC00511 Acts as an Oncogene in Colorectal Cancer via Sponging miR-29c-3p to Upregulate NFIA |
title_sort |
lncrna linc00511 acts as an oncogene in colorectal cancer via sponging mir-29c-3p to upregulate nfia |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2021-01-01 |
description |
Yu Hu,1,* Ying Zhang,2,* Meng Ding,2 Ruisi Xu2 1Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, People’s Republic of China; 2Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, People’s Republic of China*These authors contributed equally to this workCorrespondence: Meng Ding; Ruisi XuChina-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, People’s Republic of ChinaEmail dingmeng@jlu.edu.cn; xuruisi@jlu.edu.cnBackground: Colorectal cancer (CRC), characterized by high mortality and incidence rate, is one of the most common types of rectum tumors in the gastrointestinal tract worldwide. An increasing number of investigations indicated that long noncoding RNAs (lncRNAs) have been implicated in the growth of a wide range of cancers. Although it has obtained general acceptance that lncRNA LINC00511 plays a significant role in numerous cancers, the regulatory mechanism of LINC00511 in CRC still needs to be explored.Materials and Methods: Bioinformatics analysis and a wide range of experiments of sphere formation assay, cell proliferation assay, RT-qPCR, colony formation assay, Transwell assay and Western blot assays investigated the function and mechanism of LINC00511 in CRC tissues and cells.Results: Our results manifested that the expression level of LINC00511 was obviously upregulated in CRC tissues and cells and it accelerated CRC development through facilitating cell proliferation, metastasis and stemness. Molecular mechanism exploration uncovered that LINC00511 acted as a ceRNA competing with NFIA to bind with miR-29c-3p. Through rescue experiments, we discovered that NFIA upregulation partly counteracted the inhibitive effect induced by LINC00511 silencing on CRC progression.Conclusion: These results revealed that LINC00511 participated in the progression of CRC by targeting the LINC00511/miR-29c-3p/NFIA axis, indicating that LINC00511 may be a potential therapeutic target for CRC treatment.Keywords: LINC00511, miR-29c-3p, NFIA, colorectal cancer |
topic |
linc00511 mir-29c-3p nfia colorectal cancer |
url |
https://www.dovepress.com/lncrna-linc00511-acts-as-an-oncogene-in-colorectal-cancer-via-sponging-peer-reviewed-article-OTT |
work_keys_str_mv |
AT huy lncrnalinc00511actsasanoncogeneincolorectalcancerviaspongingmir29c3ptoupregulatenfia AT zhangy lncrnalinc00511actsasanoncogeneincolorectalcancerviaspongingmir29c3ptoupregulatenfia AT dingm lncrnalinc00511actsasanoncogeneincolorectalcancerviaspongingmir29c3ptoupregulatenfia AT xur lncrnalinc00511actsasanoncogeneincolorectalcancerviaspongingmir29c3ptoupregulatenfia |
_version_ |
1724347786974986240 |