Effects of Sphaeropsis Blight on Rhizosphere Soil Bacterial Community Structure and Soil Physicochemical Properties of <i>Pinus sylvestris</i> var. <i>mongolica</i> in Zhanggutai, China

• Main Authors: Saiyaremu Halifu, Xun Deng, Xiaoshuang Song, Yuning An, Ruiqing Song
• Format: Article
• Language: English
• Published: MDPI AG 2019-10-01
• Series: Forests
• Subjects: <i>pinus sylvestris</i> var. <i>mongolica</i>; rhizosphere soil; bacterial diversity; shoot blight pathology; miseq high-throughput sequencing
• Online Access: https://www.mdpi.com/1999-4907/10/11/954

<i>Pinus sylvestris</i> var. <i>mongolica</i> is an important tree species for ecological construction and environmental restoration owing to its rapid growth rate and excellent stress resistance. <i>Pinus sylvestris</i> var. <i>mongolica</i> sphaeropsis blight is a widespread disease caused by <i>Sphaeropsis sapinea</i>. This study was focused on non-infected (CK) and infected (SS) <i>Pinus sylvestris</i> var. <i>mongolica</i> plants in Zhanggutai area, Liaoning Province, China. Illumina high-throughput sequencing based on the templates of sequencing-by-synthesis working with reversible terminators is a widely used approach. In the present study, systematic differences in relationships among rhizosphere soil physicochemical properties, bacterial community structure, diverse bacterial genera, and alpha diversity indices between the two categories were evaluated. The current findings are as follows: (1) Shannon&#8217;s index of SS soil was significantly higher than CK, and it was significantly lower in May than July and September (<i>p</i> &lt; 0.05). (2) Non-metric multidimensional scaling (NMDS) showed a difference in bacterial community structure during May (spring), July (summer), and September. (3) At the phylum level, no significant difference was found in the bacterial genera between CK and SS soil for three seasons; however, at the genus level, there were about 19 different bacterial genera. The correlation studies between 19 different bacterial genera and environmental factors and &#945;-diversity indicated that bacterial genera of non-infected and infected <i>Pinus sylvestris</i> var. <i>mongolica</i> were distributed differently. The bacterial genera with CK were positively correlated with soil physicochemical properties, while a negative correlation was found for SS. In conclusion, the differences in nutrient and microbial community structure in the rhizosphere soil of <i>Pinus sylvestris</i> var. <i>mongolica</i> are the main causes of shoot blight disease.