Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells

Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells

Loss-of-function studies in human pluripotent stem cells (hPSCs) require efficient methodologies for lesion of genes of interest. Here, we introduce a donor-free paired gRNA-guided CRISPR/Cas9 knockout strategy (paired-KO) for efficient and rapid gene ablation in hPSCs. Through paired-KO, we succeed...

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Bibliographic Details
Main Authors: Zhongliang Liu, Yi Hui, Lei Shi, Zhenyu Chen, Xiangjie Xu, Liankai Chi, Beibei Fan, Yujiang Fang, Yang Liu, Lin Ma, Yiran Wang, Lei Xiao, Quanbin Zhang, Guohua Jin, Ling Liu, Xiaoqing Zhang
Format: Article
Language:English
Published: Elsevier 2016-09-01
Series:Stem Cell Reports
Subjects:
CRISPR/Cas9
human pluripotent stem cells
gene editing
non-coding RNA
Online Access:http://www.sciencedirect.com/science/article/pii/S221367111630145X

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http://www.sciencedirect.com/science/article/pii/S221367111630145X

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