The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer
As key cellular elements of hemostasis, platelets represent a primary target for thrombosis and bleeding management. Currently, therapeutic manipulations of platelet function (antithrombotic drugs) and count (platelet transfusion) are performed with limited or no real-time monitoring of the desired...
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doaj-df10c0bb071c44d595782f1a15127b4c2020-11-25T03:32:29ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01214117410.3390/ijms21041174ijms21041174The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet AggregometerYana Roka-Moiia0Silvia Bozzi1Chiara Ferrari2Gabriele Mantica3Annalisa Dimasi4Marco Rasponi5Andrea Santoleri6Mariangela Scavone7Filippo Consolo8Marco Cattaneo9Marvin J. Slepian10Alberto Redaelli11Departments of Medicine and Biomedical Engineering, College of Medicine, University of Arizona, Tucson, AZ 85724, USADepartment of Electronics, Information and Bioengineering, Politecnico di Milano, Milan 20133, ItalyDepartment of Electronics, Information and Bioengineering, Politecnico di Milano, Milan 20133, ItalyDepartment of Electronics, Information and Bioengineering, Politecnico di Milano, Milan 20133, ItalyDepartment of Electronics, Information and Bioengineering, Politecnico di Milano, Milan 20133, ItalyDepartment of Electronics, Information and Bioengineering, Politecnico di Milano, Milan 20133, ItalyDepartment of Electronics, Information and Bioengineering, Politecnico di Milano, Milan 20133, ItalyDivisione di Medicina, Ospedale San Paolo, Dipartimento di Scienze della Salute, Università degli Studi di Milano, Milan 20122, ItalyUniversità Vita Salute, San Raffaele, Milan 20132, ItalyDivisione di Medicina, Ospedale San Paolo, Dipartimento di Scienze della Salute, Università degli Studi di Milano, Milan 20122, ItalyDepartments of Medicine and Biomedical Engineering, College of Medicine, University of Arizona, Tucson, AZ 85724, USADepartment of Electronics, Information and Bioengineering, Politecnico di Milano, Milan 20133, ItalyAs key cellular elements of hemostasis, platelets represent a primary target for thrombosis and bleeding management. Currently, therapeutic manipulations of platelet function (antithrombotic drugs) and count (platelet transfusion) are performed with limited or no real-time monitoring of the desired outcome at the point-of-care. To address the need, we have designed and fabricated an easy-to-use, accurate, and portable impedance aggregometer called “MICELI” (MICrofluidic, ELectrical, Impedance). It improves on current platelet aggregation technology by decreasing footprint, assay complexity, and time to obtain results. The current study aimed to optimize the MICELI protocol; validate sensitivity to aggregation agonists and key blood parameters, i.e., platelet count and hematocrit; and verify the MICELI operational performance as compared to commercial impedance aggregometry. We demonstrated that the MICELI aggregometer could detect platelet aggregation in 250 μL of whole blood or platelet-rich plasma, stimulated by ADP, TRAP-6, collagen, epinephrine, and calcium ionophore. Using hirudin as blood anticoagulant allowed higher aggregation values. Aggregation values obtained by the MICELI strongly correlated with platelet count and were not affected by hematocrit. The operational performance comparison of the MICELI and the Multiplate<sup>®</sup> Analyzer demonstrated strong correlation and similar interdonor distribution of aggregation values obtained between these devices. With the proven reliability of the data obtained by the MICELI aggregometer, it can be further translated into a point-of-care diagnostic device aimed at monitoring platelet function in order to guide pharmacological hemostasis management and platelet transfusions.https://www.mdpi.com/1422-0067/21/4/1174platelet aggregationelectrical impedancewhole blood aggregometrypoint-of-careplatelet function testing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yana Roka-Moiia Silvia Bozzi Chiara Ferrari Gabriele Mantica Annalisa Dimasi Marco Rasponi Andrea Santoleri Mariangela Scavone Filippo Consolo Marco Cattaneo Marvin J. Slepian Alberto Redaelli |
spellingShingle |
Yana Roka-Moiia Silvia Bozzi Chiara Ferrari Gabriele Mantica Annalisa Dimasi Marco Rasponi Andrea Santoleri Mariangela Scavone Filippo Consolo Marco Cattaneo Marvin J. Slepian Alberto Redaelli The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer International Journal of Molecular Sciences platelet aggregation electrical impedance whole blood aggregometry point-of-care platelet function testing |
author_facet |
Yana Roka-Moiia Silvia Bozzi Chiara Ferrari Gabriele Mantica Annalisa Dimasi Marco Rasponi Andrea Santoleri Mariangela Scavone Filippo Consolo Marco Cattaneo Marvin J. Slepian Alberto Redaelli |
author_sort |
Yana Roka-Moiia |
title |
The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer |
title_short |
The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer |
title_full |
The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer |
title_fullStr |
The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer |
title_full_unstemmed |
The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer |
title_sort |
miceli (microfluidic, electrical, impedance): prototyping a point-of-care impedance platelet aggregometer |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-02-01 |
description |
As key cellular elements of hemostasis, platelets represent a primary target for thrombosis and bleeding management. Currently, therapeutic manipulations of platelet function (antithrombotic drugs) and count (platelet transfusion) are performed with limited or no real-time monitoring of the desired outcome at the point-of-care. To address the need, we have designed and fabricated an easy-to-use, accurate, and portable impedance aggregometer called “MICELI” (MICrofluidic, ELectrical, Impedance). It improves on current platelet aggregation technology by decreasing footprint, assay complexity, and time to obtain results. The current study aimed to optimize the MICELI protocol; validate sensitivity to aggregation agonists and key blood parameters, i.e., platelet count and hematocrit; and verify the MICELI operational performance as compared to commercial impedance aggregometry. We demonstrated that the MICELI aggregometer could detect platelet aggregation in 250 μL of whole blood or platelet-rich plasma, stimulated by ADP, TRAP-6, collagen, epinephrine, and calcium ionophore. Using hirudin as blood anticoagulant allowed higher aggregation values. Aggregation values obtained by the MICELI strongly correlated with platelet count and were not affected by hematocrit. The operational performance comparison of the MICELI and the Multiplate<sup>®</sup> Analyzer demonstrated strong correlation and similar interdonor distribution of aggregation values obtained between these devices. With the proven reliability of the data obtained by the MICELI aggregometer, it can be further translated into a point-of-care diagnostic device aimed at monitoring platelet function in order to guide pharmacological hemostasis management and platelet transfusions. |
topic |
platelet aggregation electrical impedance whole blood aggregometry point-of-care platelet function testing |
url |
https://www.mdpi.com/1422-0067/21/4/1174 |
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