Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin
A new procedure for the aggregation of human albumin and 99mTc-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in 99mTc-MAA were tested. Thus, 85 % methanol was used as the mobile phase in paper and ITL chro...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Serbian Chemical Society
2002-09-01
|
Series: | Journal of the Serbian Chemical Society |
Subjects: | |
Online Access: | http://www.shd.org.yu/HtDocs/SHD/Vol67/No8-9/V67-No8-9-04.pdf |
id |
doaj-df16eb6410ed41d6a6d8b125131e3648 |
---|---|
record_format |
Article |
spelling |
doaj-df16eb6410ed41d6a6d8b125131e36482020-11-24T23:21:40ZengSerbian Chemical Society Journal of the Serbian Chemical Society0352-51392002-09-01678-9573579Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin DIVNA DJOKICDRINA JANKOVICTATJANA MAKSINA new procedure for the aggregation of human albumin and 99mTc-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in 99mTc-MAA were tested. Thus, 85 % methanol was used as the mobile phase in paper and ITL chromatography with Whatman Noo 1 and ITLC-SA strips. A system of two solvents (acetone and 1 M NaCl or 0.9 % NaCl) was used for 3 MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3 MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Filtration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency (> 98.5 %) and percent radiochemical impurities of 99mTc-MAA. Determination of the particles number in a counter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000 350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes.http://www.shd.org.yu/HtDocs/SHD/Vol67/No8-9/V67-No8-9-04.pdfhuman albumin macroaggregates99mTc-labellingquality control |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
DIVNA DJOKIC DRINA JANKOVIC TATJANA MAKSIN |
spellingShingle |
DIVNA DJOKIC DRINA JANKOVIC TATJANA MAKSIN Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin Journal of the Serbian Chemical Society human albumin macroaggregates 99mTc-labelling quality control |
author_facet |
DIVNA DJOKIC DRINA JANKOVIC TATJANA MAKSIN |
author_sort |
DIVNA DJOKIC |
title |
Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin |
title_short |
Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin |
title_full |
Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin |
title_fullStr |
Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin |
title_full_unstemmed |
Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin |
title_sort |
radiochemical purity and particles number determinations of modified 99mtc-macroaggregated albumin |
publisher |
Serbian Chemical Society |
series |
Journal of the Serbian Chemical Society |
issn |
0352-5139 |
publishDate |
2002-09-01 |
description |
A new procedure for the aggregation of human albumin and 99mTc-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in 99mTc-MAA were tested. Thus, 85 % methanol was used as the mobile phase in paper and ITL chromatography with Whatman Noo 1 and ITLC-SA strips. A system of two solvents (acetone and 1 M NaCl or 0.9 % NaCl) was used for 3 MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3 MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Filtration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency (> 98.5 %) and percent radiochemical impurities of 99mTc-MAA. Determination of the particles number in a counter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000 350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes. |
topic |
human albumin macroaggregates 99mTc-labelling quality control |
url |
http://www.shd.org.yu/HtDocs/SHD/Vol67/No8-9/V67-No8-9-04.pdf |
work_keys_str_mv |
AT divnadjokic radiochemicalpurityandparticlesnumberdeterminationsofmodified99mtcmacroaggregatedalbumin AT drinajankovic radiochemicalpurityandparticlesnumberdeterminationsofmodified99mtcmacroaggregatedalbumin AT tatjanamaksin radiochemicalpurityandparticlesnumberdeterminationsofmodified99mtcmacroaggregatedalbumin |
_version_ |
1725570701617790976 |