STRUKTUR POPULASI DAN SEJARAH KOLONISASI IKAN BOTIA (Chromobotia macracanthus BLEEKER) ASAL SUMATERA DAN KALIMANTAN BERDASARKAN SEKUEN INTRON DARI GEN ALDOLASE-B
Clown loach (Chromobotia macracanthus Bleeker) or botia is an endemic species of Indonesia belonging to Cobitidae family which has restricted distribution only in Sumatra in Pangabuang, Kwanten, Batanghari, Teluk Betung, Musi riverines, and in Kalimantan found in Barito, Kahayan, Kapuas, Bongan dan...
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Universitas Gadjah Mada
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doaj-df187611a36f4c5ca309102e4a5d7cd22020-11-25T00:29:19ZindUniversitas Gadjah MadaJurnal Perikanan Universitas Gadjah Mada0853-63842502-50662008-08-0110220321210.22146/jfs.88947290STRUKTUR POPULASI DAN SEJARAH KOLONISASI IKAN BOTIA (Chromobotia macracanthus BLEEKER) ASAL SUMATERA DAN KALIMANTAN BERDASARKAN SEKUEN INTRON DARI GEN ALDOLASE-BSudarto Sudarto0Laurent Pouyaud1Ruby V. Kusuma2Komplek Perikanan NoIRD- representativesFakultas Perikanan dan Ilmu Kelautan, IPBClown loach (Chromobotia macracanthus Bleeker) or botia is an endemic species of Indonesia belonging to Cobitidae family which has restricted distribution only in Sumatra in Pangabuang, Kwanten, Batanghari, Teluk Betung, Musi riverines, and in Kalimantan found in Barito, Kahayan, Kapuas, Bongan dan Mahakam rivers. The objectives of this study are to find the genetical population structure and the history of colonization of clown loach from Kalimantan (Kapuas river) dan Sumatra (Batanghari, Musi and Tulang Bawang rivers) based on intron sequence of Aldolase B gene. Cutting fins are collected from each location and preserved in ethanol solution and stored in deep freezer before digested for DNA analysis by proteinase-K method followed by CTAB (cethyl/hexadecyl trimethyl ammonium bromide) method; then amplified by EPIC-PCR (Exon Primed Intron Crossing Polymerase Chain Reaction) techniques. PCR product were Electrophoreted and DNA bands were sequenced. The results of sequences analysis showed 8 types of alleles, 5 types for type A alleles (A-1, A-2, A-3, A-4, A-5), 2 type B alleles (B-1 and B-2) and C-1. There was a genetic difference between Sumatra and Kalimantan populations.https://journal.ugm.ac.id/jfs/article/view/8894aldolase-B gene, Chromobotia macracanthus, EPIC-PCR, intron |
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Indonesian |
format |
Article |
sources |
DOAJ |
author |
Sudarto Sudarto Laurent Pouyaud Ruby V. Kusuma |
spellingShingle |
Sudarto Sudarto Laurent Pouyaud Ruby V. Kusuma STRUKTUR POPULASI DAN SEJARAH KOLONISASI IKAN BOTIA (Chromobotia macracanthus BLEEKER) ASAL SUMATERA DAN KALIMANTAN BERDASARKAN SEKUEN INTRON DARI GEN ALDOLASE-B Jurnal Perikanan Universitas Gadjah Mada aldolase-B gene, Chromobotia macracanthus, EPIC-PCR, intron |
author_facet |
Sudarto Sudarto Laurent Pouyaud Ruby V. Kusuma |
author_sort |
Sudarto Sudarto |
title |
STRUKTUR POPULASI DAN SEJARAH KOLONISASI IKAN BOTIA (Chromobotia macracanthus BLEEKER) ASAL SUMATERA DAN KALIMANTAN BERDASARKAN SEKUEN INTRON DARI GEN ALDOLASE-B |
title_short |
STRUKTUR POPULASI DAN SEJARAH KOLONISASI IKAN BOTIA (Chromobotia macracanthus BLEEKER) ASAL SUMATERA DAN KALIMANTAN BERDASARKAN SEKUEN INTRON DARI GEN ALDOLASE-B |
title_full |
STRUKTUR POPULASI DAN SEJARAH KOLONISASI IKAN BOTIA (Chromobotia macracanthus BLEEKER) ASAL SUMATERA DAN KALIMANTAN BERDASARKAN SEKUEN INTRON DARI GEN ALDOLASE-B |
title_fullStr |
STRUKTUR POPULASI DAN SEJARAH KOLONISASI IKAN BOTIA (Chromobotia macracanthus BLEEKER) ASAL SUMATERA DAN KALIMANTAN BERDASARKAN SEKUEN INTRON DARI GEN ALDOLASE-B |
title_full_unstemmed |
STRUKTUR POPULASI DAN SEJARAH KOLONISASI IKAN BOTIA (Chromobotia macracanthus BLEEKER) ASAL SUMATERA DAN KALIMANTAN BERDASARKAN SEKUEN INTRON DARI GEN ALDOLASE-B |
title_sort |
struktur populasi dan sejarah kolonisasi ikan botia (chromobotia macracanthus bleeker) asal sumatera dan kalimantan berdasarkan sekuen intron dari gen aldolase-b |
publisher |
Universitas Gadjah Mada |
series |
Jurnal Perikanan Universitas Gadjah Mada |
issn |
0853-6384 2502-5066 |
publishDate |
2008-08-01 |
description |
Clown loach (Chromobotia macracanthus Bleeker) or botia is an endemic species of Indonesia belonging to Cobitidae family which has restricted distribution only in Sumatra in Pangabuang, Kwanten, Batanghari, Teluk Betung, Musi riverines, and in Kalimantan found in Barito, Kahayan, Kapuas, Bongan dan Mahakam rivers. The objectives of this study are to find the genetical population structure and the history of colonization of clown loach from Kalimantan (Kapuas river) dan Sumatra (Batanghari, Musi and Tulang Bawang rivers) based on intron sequence of Aldolase B gene. Cutting fins are collected from each location and preserved in ethanol solution and stored in deep freezer before digested for DNA analysis by proteinase-K method followed by CTAB (cethyl/hexadecyl trimethyl ammonium bromide) method; then amplified by EPIC-PCR (Exon Primed Intron Crossing Polymerase Chain Reaction) techniques. PCR product were Electrophoreted and DNA bands were sequenced. The results of sequences analysis showed 8 types of alleles, 5 types for type A alleles (A-1, A-2, A-3, A-4, A-5), 2 type B alleles (B-1 and B-2) and C-1. There was a genetic difference between Sumatra and Kalimantan populations. |
topic |
aldolase-B gene, Chromobotia macracanthus, EPIC-PCR, intron |
url |
https://journal.ugm.ac.id/jfs/article/view/8894 |
work_keys_str_mv |
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