Razor scrape assay, an alternative variation to wound and healing assays

• Main Authors: Jesús Antonio Lara-Reyes, Mariana Giezi Jimenez-Buendia, Gonzalo Emiliano Aranda-Abreu, Deissy Herrera-Covarrubias, Clara Luz Sampieri, Arnoldo Aquino-Galvez, Jorge Manzo-Denes, María Elena Hernández-Aguilar, Fausto Rojas-Durán
• Format: Article
• Language: English
• Published: Elsevier 2020-01-01
• Series: MethodsX
• Subjects: Cell culture techniques; Cell migration assays; Cell movement
• Online Access: http://www.sciencedirect.com/science/article/pii/S2215016120303551

Cell migration is the process by which cells move through tissues, and it is crucial to carry out a wide variety of physiological and pathological processes. The study methods to evaluate cell migration are very useful tools for biomedical research. Among these methods, the wound and healing assay is one of the simplest, most economical and is widely used in research. However, one of its disadvantages is that the width and shape of the wound can vary among experimental samples since the scraping is carried out manually, representing a difficult variable to control. In the present article a variant of the razor scrape assay is addressed, which eliminates this variation in the width of the wound, thus facilitating the measurement and comparison using the total area of cell migration. • A method that can be carried out under standard culture conditions. • Avoids the disadvantage of variation in width and shape of the wound. • It constitutes a simple, cheap option and multiple advantages over the traditional method.