Water and Collagen Content Are High in Pancreatic Cancer: Implications for Quantitative Metabolic Imaging
In magnetic resonance metabolic imaging, signal from the water content is frequently used for normalization to derive quantitative or semi-quantitative values of metabolites in vivo or ex vivo tumors and tissues. Ex vivo high-resolution metabolic characterization of tumors with magnetic resonance sp...
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doaj-df3f5df137bf46df9a7f97537bcc475e2021-01-27T09:13:05ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-01-011010.3389/fonc.2020.599204599204Water and Collagen Content Are High in Pancreatic Cancer: Implications for Quantitative Metabolic ImagingMarie-France Penet0Marie-France Penet1Samata Kakkad2Flonné Wildes3Zaver M. Bhujwalla4Zaver M. Bhujwalla5Zaver M. Bhujwalla6Division of Cancer Imaging Research, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United StatesSidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDivision of Cancer Imaging Research, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDivision of Cancer Imaging Research, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDivision of Cancer Imaging Research, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United StatesSidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDepartment of Radiation Oncology and Molecular Radiation Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, United StatesIn magnetic resonance metabolic imaging, signal from the water content is frequently used for normalization to derive quantitative or semi-quantitative values of metabolites in vivo or ex vivo tumors and tissues. Ex vivo high-resolution metabolic characterization of tumors with magnetic resonance spectroscopy (MRS) provides valuable information that can be used to drive the development of noninvasive MRS biomarkers and to identify metabolic therapeutic targets. Variability in the water content between tumor and normal tissue can result in over or underestimation of metabolite concentrations when assuming a constant water content. Assuming a constant water content can lead to masking of differences between malignant and normal tissues both in vivo and ex vivo. There is a critical need to develop biomarkers to detect pancreatic cancer and to develop novel treatments. Our purpose here was to determine the differences in water content between pancreatic tumors and normal pancreatic tissue as well as other organs to accurately quantify metabolic differences when using the water signal for normalization. Our data identify the importance of factoring the differences in water content between tumors and organs. High-resolution proton spectra of tumors and pancreatic tissue extracts normalized to the water signal, assuming similar water content, did not reflect the significantly increased total choline observed in tumors in vivo without factoring the differences in water content. We identified significant differences in the collagen 1 content between Panc1 and BxPC3 pancreatic tumors and the pancreas that can contribute to the differences in water content that were observed.https://www.frontiersin.org/articles/10.3389/fonc.2020.599204/fullpancreatic cancertotal cholineMRScollagenwater content |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marie-France Penet Marie-France Penet Samata Kakkad Flonné Wildes Zaver M. Bhujwalla Zaver M. Bhujwalla Zaver M. Bhujwalla |
spellingShingle |
Marie-France Penet Marie-France Penet Samata Kakkad Flonné Wildes Zaver M. Bhujwalla Zaver M. Bhujwalla Zaver M. Bhujwalla Water and Collagen Content Are High in Pancreatic Cancer: Implications for Quantitative Metabolic Imaging Frontiers in Oncology pancreatic cancer total choline MRS collagen water content |
author_facet |
Marie-France Penet Marie-France Penet Samata Kakkad Flonné Wildes Zaver M. Bhujwalla Zaver M. Bhujwalla Zaver M. Bhujwalla |
author_sort |
Marie-France Penet |
title |
Water and Collagen Content Are High in Pancreatic Cancer: Implications for Quantitative Metabolic Imaging |
title_short |
Water and Collagen Content Are High in Pancreatic Cancer: Implications for Quantitative Metabolic Imaging |
title_full |
Water and Collagen Content Are High in Pancreatic Cancer: Implications for Quantitative Metabolic Imaging |
title_fullStr |
Water and Collagen Content Are High in Pancreatic Cancer: Implications for Quantitative Metabolic Imaging |
title_full_unstemmed |
Water and Collagen Content Are High in Pancreatic Cancer: Implications for Quantitative Metabolic Imaging |
title_sort |
water and collagen content are high in pancreatic cancer: implications for quantitative metabolic imaging |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2021-01-01 |
description |
In magnetic resonance metabolic imaging, signal from the water content is frequently used for normalization to derive quantitative or semi-quantitative values of metabolites in vivo or ex vivo tumors and tissues. Ex vivo high-resolution metabolic characterization of tumors with magnetic resonance spectroscopy (MRS) provides valuable information that can be used to drive the development of noninvasive MRS biomarkers and to identify metabolic therapeutic targets. Variability in the water content between tumor and normal tissue can result in over or underestimation of metabolite concentrations when assuming a constant water content. Assuming a constant water content can lead to masking of differences between malignant and normal tissues both in vivo and ex vivo. There is a critical need to develop biomarkers to detect pancreatic cancer and to develop novel treatments. Our purpose here was to determine the differences in water content between pancreatic tumors and normal pancreatic tissue as well as other organs to accurately quantify metabolic differences when using the water signal for normalization. Our data identify the importance of factoring the differences in water content between tumors and organs. High-resolution proton spectra of tumors and pancreatic tissue extracts normalized to the water signal, assuming similar water content, did not reflect the significantly increased total choline observed in tumors in vivo without factoring the differences in water content. We identified significant differences in the collagen 1 content between Panc1 and BxPC3 pancreatic tumors and the pancreas that can contribute to the differences in water content that were observed. |
topic |
pancreatic cancer total choline MRS collagen water content |
url |
https://www.frontiersin.org/articles/10.3389/fonc.2020.599204/full |
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