Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins
Adipose tissue-derived mesenchymal stem cells (ADMSCs) are promising candidates for regenerative medicine, as they have good cell yield and can differentiate into several cell lines. When induced to the neuronal differentiation, they form neurospheres composed of neural precursors (NPs) that can be...
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doaj-df44e70d0a3b4890862602f8eb2af7f72021-09-26T00:40:22ZengMDPI AGMembranes2077-03752021-09-011168768710.3390/membranes11090687Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT ProteinsNathalia Barth de Oliveira0Ana Carolina Irioda1Priscila Elias Ferreira Stricker2Bassam Felipe Mogharbel3Nádia Nascimento da Rosa4Dilcele Silva Moreira Dziedzic5Katherine Athayde Teixeira de Carvalho6Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research and Pequeno Príncipe Faculties, Curitiba 80240-020, Paraná, BrazilAdvanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research and Pequeno Príncipe Faculties, Curitiba 80240-020, Paraná, BrazilAdvanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research and Pequeno Príncipe Faculties, Curitiba 80240-020, Paraná, BrazilAdvanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research and Pequeno Príncipe Faculties, Curitiba 80240-020, Paraná, BrazilAdvanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research and Pequeno Príncipe Faculties, Curitiba 80240-020, Paraná, BrazilAdvanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research and Pequeno Príncipe Faculties, Curitiba 80240-020, Paraná, BrazilAdvanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research and Pequeno Príncipe Faculties, Curitiba 80240-020, Paraná, BrazilAdipose tissue-derived mesenchymal stem cells (ADMSCs) are promising candidates for regenerative medicine, as they have good cell yield and can differentiate into several cell lines. When induced to the neuronal differentiation, they form neurospheres composed of neural precursors (NPs) that can be an alternative in treating neurodegenerative diseases. This study aimed to characterize NPs from neurospheres obtained after seeding ADMSCs on a natural polyisoprene-based membrane. The ADMSCs were isolated from adipose tissue by enzymatic dissociation, were subjected to trilineage differentiation, and were characterized by flow cytometry for specific ADMSC surface markers. For neuronal differentiation, the cells were seeded on polystyrene flasks coated with the membrane and were characterized by immunocytochemistry and RT-PCR. The results demonstrated that the isolated cells showed characteristics of ADMSCs. At 15 to 25 days, ADMSCs seeded on the natural membrane developed neurospheres. Then, after dissociation, the cells demonstrated characteristic neuronal markers expressed on NPs: nestin, ß-III tubulin, GFAP, NeuN, and the YAP1/AMOT in the cytoplasm. In conclusion, it was demonstrated that this membrane differentiates the ADMSCs to NPs without any induction factors, and suggests that their differentiation mechanisms are related to mechanotransduction regulated by the YAP and AMOT proteins.https://www.mdpi.com/2077-0375/11/9/687adipose-derived mesenchymal stem cellsmembraneneural precursorpolyisopreneYAPangiomotin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nathalia Barth de Oliveira Ana Carolina Irioda Priscila Elias Ferreira Stricker Bassam Felipe Mogharbel Nádia Nascimento da Rosa Dilcele Silva Moreira Dziedzic Katherine Athayde Teixeira de Carvalho |
spellingShingle |
Nathalia Barth de Oliveira Ana Carolina Irioda Priscila Elias Ferreira Stricker Bassam Felipe Mogharbel Nádia Nascimento da Rosa Dilcele Silva Moreira Dziedzic Katherine Athayde Teixeira de Carvalho Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins Membranes adipose-derived mesenchymal stem cells membrane neural precursor polyisoprene YAP angiomotin |
author_facet |
Nathalia Barth de Oliveira Ana Carolina Irioda Priscila Elias Ferreira Stricker Bassam Felipe Mogharbel Nádia Nascimento da Rosa Dilcele Silva Moreira Dziedzic Katherine Athayde Teixeira de Carvalho |
author_sort |
Nathalia Barth de Oliveira |
title |
Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins |
title_short |
Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins |
title_full |
Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins |
title_fullStr |
Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins |
title_full_unstemmed |
Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins |
title_sort |
natural membrane differentiates human adipose-derived mesenchymal stem cells to neurospheres by mechanotransduction related to yap and amot proteins |
publisher |
MDPI AG |
series |
Membranes |
issn |
2077-0375 |
publishDate |
2021-09-01 |
description |
Adipose tissue-derived mesenchymal stem cells (ADMSCs) are promising candidates for regenerative medicine, as they have good cell yield and can differentiate into several cell lines. When induced to the neuronal differentiation, they form neurospheres composed of neural precursors (NPs) that can be an alternative in treating neurodegenerative diseases. This study aimed to characterize NPs from neurospheres obtained after seeding ADMSCs on a natural polyisoprene-based membrane. The ADMSCs were isolated from adipose tissue by enzymatic dissociation, were subjected to trilineage differentiation, and were characterized by flow cytometry for specific ADMSC surface markers. For neuronal differentiation, the cells were seeded on polystyrene flasks coated with the membrane and were characterized by immunocytochemistry and RT-PCR. The results demonstrated that the isolated cells showed characteristics of ADMSCs. At 15 to 25 days, ADMSCs seeded on the natural membrane developed neurospheres. Then, after dissociation, the cells demonstrated characteristic neuronal markers expressed on NPs: nestin, ß-III tubulin, GFAP, NeuN, and the YAP1/AMOT in the cytoplasm. In conclusion, it was demonstrated that this membrane differentiates the ADMSCs to NPs without any induction factors, and suggests that their differentiation mechanisms are related to mechanotransduction regulated by the YAP and AMOT proteins. |
topic |
adipose-derived mesenchymal stem cells membrane neural precursor polyisoprene YAP angiomotin |
url |
https://www.mdpi.com/2077-0375/11/9/687 |
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