Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent Extractor
Effects of varying temperatures with constant pressure of solvent on extraction efficiency of two chemically different alkaloids were studied. Camptothecin (CPT) from stem of Nothapodytes nimmoniana (Grah.) Mabb. and piperine from the fruits of Piper nigrum L. were extracted using Accelerated Solven...
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doaj-df60781241c3476190a9d5027b9f1a792020-11-24T21:32:32ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/932036932036Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent ExtractorVinayak Upadhya0Sandeep R. Pai1Ajay K. Sharma2Harsha V. Hegde3Sanjiva D. Kholkute4Rajesh K. Joshi5Regional Medical Research Centre, Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum, Karnataka 590 010, IndiaRegional Medical Research Centre, Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum, Karnataka 590 010, IndiaRegional Medical Research Centre, Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum, Karnataka 590 010, IndiaRegional Medical Research Centre, Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum, Karnataka 590 010, IndiaRegional Medical Research Centre, Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum, Karnataka 590 010, IndiaRegional Medical Research Centre, Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum, Karnataka 590 010, IndiaEffects of varying temperatures with constant pressure of solvent on extraction efficiency of two chemically different alkaloids were studied. Camptothecin (CPT) from stem of Nothapodytes nimmoniana (Grah.) Mabb. and piperine from the fruits of Piper nigrum L. were extracted using Accelerated Solvent Extractor (ASE). Three cycles of extraction for a particular sample cell at a given temperature assured complete extraction. CPT and piperine were determined and quantified by using a simple and efficient UFLC-PDA (245 and 343 nm) method. Temperature increased efficiency of extraction to yield higher amount of CPT, whereas temperature had diminutive effect on yield of piperine. Maximum yield for CPT was achieved at 80∘C and for piperine at 40∘C. Thus, the study determines compound specific extraction of CPT from N. nimmoniana and piperine from P. nigrum using ASE method. The present study indicates the use of this method for simple, fast, and accurate extraction of the compound of interest.http://dx.doi.org/10.1155/2014/932036 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vinayak Upadhya Sandeep R. Pai Ajay K. Sharma Harsha V. Hegde Sanjiva D. Kholkute Rajesh K. Joshi |
spellingShingle |
Vinayak Upadhya Sandeep R. Pai Ajay K. Sharma Harsha V. Hegde Sanjiva D. Kholkute Rajesh K. Joshi Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent Extractor Journal of Analytical Methods in Chemistry |
author_facet |
Vinayak Upadhya Sandeep R. Pai Ajay K. Sharma Harsha V. Hegde Sanjiva D. Kholkute Rajesh K. Joshi |
author_sort |
Vinayak Upadhya |
title |
Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent Extractor |
title_short |
Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent Extractor |
title_full |
Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent Extractor |
title_fullStr |
Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent Extractor |
title_full_unstemmed |
Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent Extractor |
title_sort |
compound specific extraction of camptothecin from nothapodytes nimmoniana and piperine from piper nigrum using accelerated solvent extractor |
publisher |
Hindawi Limited |
series |
Journal of Analytical Methods in Chemistry |
issn |
2090-8865 2090-8873 |
publishDate |
2014-01-01 |
description |
Effects of varying temperatures with constant pressure of solvent on extraction efficiency of two chemically different alkaloids were studied. Camptothecin (CPT) from stem of Nothapodytes nimmoniana (Grah.) Mabb. and piperine from the fruits of Piper nigrum L. were extracted using Accelerated Solvent Extractor (ASE). Three cycles of extraction for a particular sample cell at a given temperature assured complete extraction. CPT and piperine were determined and quantified by using a simple and efficient UFLC-PDA (245 and 343 nm) method. Temperature increased efficiency of extraction to yield higher amount of CPT, whereas temperature had diminutive effect on yield of piperine. Maximum yield for CPT was achieved at 80∘C and for piperine at 40∘C. Thus, the study determines compound specific extraction of CPT from N. nimmoniana and piperine from P. nigrum using ASE method. The present study indicates the use of this method for simple, fast, and accurate extraction of the compound of interest. |
url |
http://dx.doi.org/10.1155/2014/932036 |
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