Syntrophic Partners Enhance Growth and Respiratory Dehalogenation of Hexachlorobenzene by Dehalococcoides mccartyi Strain CBDB1
This study investigated syntrophic interactions between chlorinated benzene respiring Dehalococcoides mccartyi strain CBDB1 and fermenting partners (Desulfovibrio vulgaris, Syntrophobacter fumaroxidans, and Geobacter lovleyi) during hexachlorobenzene respiration. Dechlorination rates in syntrophic c...
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doaj-df7d6f8ad8e247d3965df0f1bcf23c432020-11-24T23:39:28ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-08-01910.3389/fmicb.2018.01927383056Syntrophic Partners Enhance Growth and Respiratory Dehalogenation of Hexachlorobenzene by Dehalococcoides mccartyi Strain CBDB1Anh T. T. Chau0Anh T. T. Chau1Matthew Lee2Lorenz Adrian3Michael J. Manefield4Michael J. Manefield5College of Agriculture and Applied Biology, Cantho University, Can Tho, VietnamSchool of Civil and Environmental Engineering, University of New South Wales, Sydney, NSW, AustraliaSchool of Civil and Environmental Engineering, University of New South Wales, Sydney, NSW, AustraliaDepartment Isotope Biogeochemistry, Helmholtz Centre for Environmental Research – UFZ, Leipzig, GermanySchool of Civil and Environmental Engineering, University of New South Wales, Sydney, NSW, AustraliaSchool of Chemical Engineering, University of New South Wales, Sydney, NSW, AustraliaThis study investigated syntrophic interactions between chlorinated benzene respiring Dehalococcoides mccartyi strain CBDB1 and fermenting partners (Desulfovibrio vulgaris, Syntrophobacter fumaroxidans, and Geobacter lovleyi) during hexachlorobenzene respiration. Dechlorination rates in syntrophic co-cultures were enhanced 2-3 fold compared to H2 fed CBDB1 pure cultures (0.23 ± 0.04 μmol Cl− day−1). Syntrophic partners were also able to supply cobalamins to CBDB1, albeit with 3–10 fold lower resultant dechlorination activity compared to cultures receiving exogenous cyanocobalamin. Strain CBDB1 pure cultures accumulated ~1 μmol of carbon monoxide per 87.5 μmol Cl− released during hexachlorobenzene respiration resulting in decreases in dechlorination activity. The syntrophic partners investigated were shown to consume carbon monoxide generated by CBDB1, thus relieving carbon monoxide autotoxicity. Accumulation of lesser chlorinated chlorobenzene congeners (1,3- and 1,4-dichlorobenzene and 1,3,5-trichlorobenzene) also inhibited dechlorination activity and their removal from the headspace through adsorption to granular activated carbon was shown to restore activity. Proteomic analysis revealed co-culturing strain CBDB1 with Geobacter lovleyi upregulated CBDB1 genes associated with reductive dehalogenases, hydrogenases, formate dehydrogenase, and ribosomal proteins. These data provide insight into CBDB1 ecology and inform strategies for application of CBDB1 in ex situ hexachlorobenzene destruction technologies.https://www.frontiersin.org/article/10.3389/fmicb.2018.01927/fullsyntrophyhexachlorobenzeneorganohalide respirationDehalococcoides mccartyi strain CBDB1carbon monoxide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anh T. T. Chau Anh T. T. Chau Matthew Lee Lorenz Adrian Michael J. Manefield Michael J. Manefield |
spellingShingle |
Anh T. T. Chau Anh T. T. Chau Matthew Lee Lorenz Adrian Michael J. Manefield Michael J. Manefield Syntrophic Partners Enhance Growth and Respiratory Dehalogenation of Hexachlorobenzene by Dehalococcoides mccartyi Strain CBDB1 Frontiers in Microbiology syntrophy hexachlorobenzene organohalide respiration Dehalococcoides mccartyi strain CBDB1 carbon monoxide |
author_facet |
Anh T. T. Chau Anh T. T. Chau Matthew Lee Lorenz Adrian Michael J. Manefield Michael J. Manefield |
author_sort |
Anh T. T. Chau |
title |
Syntrophic Partners Enhance Growth and Respiratory Dehalogenation of Hexachlorobenzene by Dehalococcoides mccartyi Strain CBDB1 |
title_short |
Syntrophic Partners Enhance Growth and Respiratory Dehalogenation of Hexachlorobenzene by Dehalococcoides mccartyi Strain CBDB1 |
title_full |
Syntrophic Partners Enhance Growth and Respiratory Dehalogenation of Hexachlorobenzene by Dehalococcoides mccartyi Strain CBDB1 |
title_fullStr |
Syntrophic Partners Enhance Growth and Respiratory Dehalogenation of Hexachlorobenzene by Dehalococcoides mccartyi Strain CBDB1 |
title_full_unstemmed |
Syntrophic Partners Enhance Growth and Respiratory Dehalogenation of Hexachlorobenzene by Dehalococcoides mccartyi Strain CBDB1 |
title_sort |
syntrophic partners enhance growth and respiratory dehalogenation of hexachlorobenzene by dehalococcoides mccartyi strain cbdb1 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-08-01 |
description |
This study investigated syntrophic interactions between chlorinated benzene respiring Dehalococcoides mccartyi strain CBDB1 and fermenting partners (Desulfovibrio vulgaris, Syntrophobacter fumaroxidans, and Geobacter lovleyi) during hexachlorobenzene respiration. Dechlorination rates in syntrophic co-cultures were enhanced 2-3 fold compared to H2 fed CBDB1 pure cultures (0.23 ± 0.04 μmol Cl− day−1). Syntrophic partners were also able to supply cobalamins to CBDB1, albeit with 3–10 fold lower resultant dechlorination activity compared to cultures receiving exogenous cyanocobalamin. Strain CBDB1 pure cultures accumulated ~1 μmol of carbon monoxide per 87.5 μmol Cl− released during hexachlorobenzene respiration resulting in decreases in dechlorination activity. The syntrophic partners investigated were shown to consume carbon monoxide generated by CBDB1, thus relieving carbon monoxide autotoxicity. Accumulation of lesser chlorinated chlorobenzene congeners (1,3- and 1,4-dichlorobenzene and 1,3,5-trichlorobenzene) also inhibited dechlorination activity and their removal from the headspace through adsorption to granular activated carbon was shown to restore activity. Proteomic analysis revealed co-culturing strain CBDB1 with Geobacter lovleyi upregulated CBDB1 genes associated with reductive dehalogenases, hydrogenases, formate dehydrogenase, and ribosomal proteins. These data provide insight into CBDB1 ecology and inform strategies for application of CBDB1 in ex situ hexachlorobenzene destruction technologies. |
topic |
syntrophy hexachlorobenzene organohalide respiration Dehalococcoides mccartyi strain CBDB1 carbon monoxide |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.01927/full |
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