Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.)

• Main Authors: Abhijit Daspute, B. Fakrudin
• Format: Article
• Language: English
• Published: Hanrimwon Publishing Company 2015-03-01
• Series: The Plant Pathology Journal
• Subjects: pigeonpea; random DNA markers coupling and repulsion phase; sterility mosaic disease
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/4356603

Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F₂ mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7₄₁₄) and a repulsion phase marker (IABTPPN7₉₈₃) co-segregating with PSMD reaction. Screening of 325 F₂ population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7₉₈₃, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7₄₁₄ did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7₄₁₄ (P<0.0001) recorded a significant association with the PSMD resistance and explained a phenotypic variance of 31 and 36% respectively in F₂ population. The repulsion phase marker, IABTPPN7₉₈₃, could be of use in Marker-Assisted Selection (MAS) in the PPSMV resistance breeding programmes of pigeonpea.