Evaluation of sorghum [Sorghum bicolor (L.)] reference genes in various tissues and under abiotic stress conditions for quantitative real-time PCR data normalization

Evaluation of sorghum [Sorghum bicolor (L.)] reference genes in various tissues and under abiotic stress conditions for quantitative real-time PCR data normalization

Accurate and reliable gene expression data from qPCR depends on stable reference gene expression for potential gene functional analyses. In this study, 15 reference genes were selected and analyzed in various sample sets including abiotic stress treatments (salt, cold, water stress, heat and abscisi...

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Bibliographic Details
Main Authors: Palakolanu eSudhakar Reddy, Dumbala eSrinivas Reddy, Sivasakthi eKaliamoorthy, Pooja eBhatnagar-Mathur, Vincent eVadez, Kiran K Sharma
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-04-01
Series:Frontiers in Plant Science
Subjects:
qPCR
normalization
reference gene
Sorghum bicolor
Gene expression stability
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Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00529/full

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