Surface-Modified Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits
Poly(<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and...
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doaj-df882043441445988bb0509809dfbd562020-11-25T02:48:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-01217541754110.3390/ijms21207541Surface-Modified Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on RabbitsMałgorzata Krok-Borkowicz0Katarzyna Reczyńska1Łucja Rumian2Elżbieta Menaszek3Maciej Orzelski4Piotr Malisz5Piotr Silmanowicz6Piotr Dobrzyński7Elżbieta Pamuła8Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH—University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH—University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH—University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandDepartment of Cytobiology, Faculty of Pharmacy, Collegium Medicum, Jagiellonian University, ul. Medyczna 9, 30-688 Kraków, PolandDepartment and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences, ul. Głęboka 30, 20-612 Lublin, PolandDepartment of Electroradiology, Collegium Medicum, Faculty of Health Science, Jagiellonian University, ul. Michałowskiego 12, 31-126 Kraków, PolandDepartment and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences, ul. Głęboka 30, 20-612 Lublin, PolandCentre of Polymer and Carbon Materials, Polish Academy of Sciences, ul. Curie-Sklodowskiej 34, 41-800 Zabrze, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH—University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandPoly(<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and bone tissue regeneration potential. The scaffolds were fabricated using solvent casting/particulate leaching method. Their total porosity was 85% and the pore size was in the range of 250–320 µm. The physico-chemical properties of the scaffolds were evaluated using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), sessile drop, and compression tests. Three types of the scaffolds (unmodified PLGA, PLGA/coll, and PLGA/HAp) were implanted into the defects created in New Zealand rabbit femoral trochlears; empty defect acted as control. Samples were extracted after 1, 4, 12, and 26 weeks from the implantation, evaluated using micro-computed tomography (µCT), and stained by Masson–Goldner and hematoxylin-eosin. The results showed that the proposed method is suitable for fabrication of highly porous PLGA scaffolds. Effective deposition of both coll and HAp was confirmed on all surfaces of the pores through the entire scaffold volume. In the in vivo model, PLGA and PLGA/HAp scaffolds enhanced tissue ingrowth as shown by histological and morphometric analyses. Bone formation was the highest for PLGA/HAp scaffolds as evidenced by µCT. Neo-tissue formation in the defect site was well correlated with degradation kinetics of the scaffold material. Interestingly, around PLGA/coll extensive inflammation and inhibited tissue healing were detected, presumably due to immunological response of the host towards collagen of bovine origin. To summarize, PLGA scaffolds modified with HAp are the most promising materials for bone tissue regeneration.https://www.mdpi.com/1422-0067/21/20/7541PLGAscaffoldscollagenhydroxyapatitein vivo tests |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Małgorzata Krok-Borkowicz Katarzyna Reczyńska Łucja Rumian Elżbieta Menaszek Maciej Orzelski Piotr Malisz Piotr Silmanowicz Piotr Dobrzyński Elżbieta Pamuła |
spellingShingle |
Małgorzata Krok-Borkowicz Katarzyna Reczyńska Łucja Rumian Elżbieta Menaszek Maciej Orzelski Piotr Malisz Piotr Silmanowicz Piotr Dobrzyński Elżbieta Pamuła Surface-Modified Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits International Journal of Molecular Sciences PLGA scaffolds collagen hydroxyapatite in vivo tests |
author_facet |
Małgorzata Krok-Borkowicz Katarzyna Reczyńska Łucja Rumian Elżbieta Menaszek Maciej Orzelski Piotr Malisz Piotr Silmanowicz Piotr Dobrzyński Elżbieta Pamuła |
author_sort |
Małgorzata Krok-Borkowicz |
title |
Surface-Modified Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits |
title_short |
Surface-Modified Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits |
title_full |
Surface-Modified Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits |
title_fullStr |
Surface-Modified Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits |
title_full_unstemmed |
Surface-Modified Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits |
title_sort |
surface-modified poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) scaffolds for the treatment of osteochondral critical size defects—in vivo studies on rabbits |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-10-01 |
description |
Poly(<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and bone tissue regeneration potential. The scaffolds were fabricated using solvent casting/particulate leaching method. Their total porosity was 85% and the pore size was in the range of 250–320 µm. The physico-chemical properties of the scaffolds were evaluated using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), sessile drop, and compression tests. Three types of the scaffolds (unmodified PLGA, PLGA/coll, and PLGA/HAp) were implanted into the defects created in New Zealand rabbit femoral trochlears; empty defect acted as control. Samples were extracted after 1, 4, 12, and 26 weeks from the implantation, evaluated using micro-computed tomography (µCT), and stained by Masson–Goldner and hematoxylin-eosin. The results showed that the proposed method is suitable for fabrication of highly porous PLGA scaffolds. Effective deposition of both coll and HAp was confirmed on all surfaces of the pores through the entire scaffold volume. In the in vivo model, PLGA and PLGA/HAp scaffolds enhanced tissue ingrowth as shown by histological and morphometric analyses. Bone formation was the highest for PLGA/HAp scaffolds as evidenced by µCT. Neo-tissue formation in the defect site was well correlated with degradation kinetics of the scaffold material. Interestingly, around PLGA/coll extensive inflammation and inhibited tissue healing were detected, presumably due to immunological response of the host towards collagen of bovine origin. To summarize, PLGA scaffolds modified with HAp are the most promising materials for bone tissue regeneration. |
topic |
PLGA scaffolds collagen hydroxyapatite in vivo tests |
url |
https://www.mdpi.com/1422-0067/21/20/7541 |
work_keys_str_mv |
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