Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus s...
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Format: | Article |
Language: | English |
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Elsevier
2021-03-01
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Series: | Veterinary and Animal Science |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2451943X20300764 |
id |
doaj-df8d0338a62a4bc1a45212f8bd174d4c |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rafael Delpiazzo Maila Barcellos Sofía Barros Laura Betancor Martín Fraga Jorge Gil Gregorio Iraola Claudia Morsella Fernando Paolicchi Ruben Pérez Franklin Riet-Correa Margarita Sanguinetti Alfonso Silva Caroline da Silva Silveira Lucía Calleros |
spellingShingle |
Rafael Delpiazzo Maila Barcellos Sofía Barros Laura Betancor Martín Fraga Jorge Gil Gregorio Iraola Claudia Morsella Fernando Paolicchi Ruben Pérez Franklin Riet-Correa Margarita Sanguinetti Alfonso Silva Caroline da Silva Silveira Lucía Calleros Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence Veterinary and Animal Science Campylobacter fetus Bovine genital campylobacteriosis qPCR Molecular diagnosis |
author_facet |
Rafael Delpiazzo Maila Barcellos Sofía Barros Laura Betancor Martín Fraga Jorge Gil Gregorio Iraola Claudia Morsella Fernando Paolicchi Ruben Pérez Franklin Riet-Correa Margarita Sanguinetti Alfonso Silva Caroline da Silva Silveira Lucía Calleros |
author_sort |
Rafael Delpiazzo |
title |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
title_short |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
title_full |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
title_fullStr |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
title_full_unstemmed |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
title_sort |
accurate and fast identification of campylobacter fetus in bulls by real-time pcr targeting a 16s rrna gene sequence |
publisher |
Elsevier |
series |
Veterinary and Animal Science |
issn |
2451-943X |
publishDate |
2021-03-01 |
description |
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. |
topic |
Campylobacter fetus Bovine genital campylobacteriosis qPCR Molecular diagnosis |
url |
http://www.sciencedirect.com/science/article/pii/S2451943X20300764 |
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doaj-df8d0338a62a4bc1a45212f8bd174d4c2021-03-07T04:30:03ZengElsevierVeterinary and Animal Science2451-943X2021-03-0111100163Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequenceRafael Delpiazzo0Maila Barcellos1Sofía Barros2Laura Betancor3Martín Fraga4Jorge Gil5Gregorio Iraola6Claudia Morsella7Fernando Paolicchi8Ruben Pérez9Franklin Riet-Correa10Margarita Sanguinetti11Alfonso Silva12Caroline da Silva Silveira13Lucía Calleros14Departamento de Salud de los Sistemas Pecuarios, Facultad de Veterinaria, Universidad de la República Oriental del Uruguay, Estación Experimental ''Dr. Mario A. Cassinoni'', Ruta 3 Km. 363, Paysandú, UruguaySección Genética Evolutiva, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Iguá 4225, Montevideo, UruguaySección Genética Evolutiva, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Iguá 4225, Montevideo, UruguayDepartamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Dr. Alfredo Navarro 3051, Montevideo, UruguayPlataforma de Investigación en Salud Animal, Estación Experimental INIA La Estanzuela. Ruta 50 Km. 11, Colonia, UruguayDepartamento de Salud de los Sistemas Pecuarios, Facultad de Veterinaria, Universidad de la República Oriental del Uruguay, Estación Experimental ''Dr. Mario A. Cassinoni'', Ruta 3 Km. 363, Paysandú, UruguayLaboratorio de Genómica Microbiana, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, Uruguay; Centro de Biología Integrativa, Facultad de Ciencias, Universidad Mayor, Camino La Pirámide 5750, Huechuraba, Santiago de Chile, Chile; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Saffron Walden CB10 1SA, United KingdomLaboratorio de Bacteriología, Estación Experimental Agropecuaria, INTA Balcarce. Ruta 226 Km. 73.5, Balcarce, Buenos Aires, ArgentinaLaboratorio de Bacteriología, Estación Experimental Agropecuaria, INTA Balcarce. Ruta 226 Km. 73.5, Balcarce, Buenos Aires, ArgentinaSección Genética Evolutiva, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Iguá 4225, Montevideo, UruguayPlataforma de Investigación en Salud Animal, Estación Experimental INIA La Estanzuela. Ruta 50 Km. 11, Colonia, UruguaySección Genética Evolutiva, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Iguá 4225, Montevideo, UruguaySección Genética Evolutiva, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Iguá 4225, Montevideo, UruguayPlataforma de Investigación en Salud Animal, Estación Experimental INIA La Estanzuela. Ruta 50 Km. 11, Colonia, UruguaySección Genética Evolutiva, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Iguá 4225, Montevideo, Uruguay; Corresponding author:Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.http://www.sciencedirect.com/science/article/pii/S2451943X20300764Campylobacter fetusBovine genital campylobacteriosisqPCRMolecular diagnosis |