Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity
Zofia Kautzka, Sandhya Clement, Ewa M Goldys, Wei Deng Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, North Ryde, Sydney, NSW, Australia Abstract: We developed light-triggered liposomes incorporating 3–5 nm hydrophobic gold nanoparticles and Rose Bengal (...
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doaj-df9051e1a5fc4cc083edf5abdceafeaa2020-11-24T21:25:58ZengDove Medical PressInternational Journal of Nanomedicine1178-20132017-02-01Volume 1296997731135Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicityKautzka ZClement SGoldys EMDeng WZofia Kautzka, Sandhya Clement, Ewa M Goldys, Wei Deng Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, North Ryde, Sydney, NSW, Australia Abstract: We developed light-triggered liposomes incorporating 3–5 nm hydrophobic gold nanoparticles and Rose Bengal (RB), a well-known photosensitizer used for photodynamic therapy. Singlet oxygen generated by these liposomes with 532 nm light illumination was characterized for varying the molar ratio of lipids and gold nanoparticles while keeping the amount of RB constant. Gold nanoparticles were found to enhance the singlet oxygen generation rate, with a maximum enhancement factor of 1.75 obtained for the molar ratio of hydrogenated soy l-α-phosphatidylcholine:1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(hexanoylamine):gold of 57:5:17 compared with liposomes loaded with RB alone. The experimental results could be explained by the local electric field enhancement caused by gold nanoparticles. We further assessed cellular cytotoxicity of gold-loaded liposomes by encapsulating an antitumor drug, doxorubicin (Dox); such Dox-loaded liposomes were applied to human colorectal cancer cells (HCT116) and exposed to light. Gold-loaded liposomes containing RB and Dox where Dox release was triggered by light were found to exhibit higher cytotoxicity compared with the liposomes loaded with RB and Dox alone. Our results indicate that gold-loaded liposomes incorporating photosensitizers may serve as improved agents in photodynamic therapy and chemotherapy. Keywords: liposomes, Rose Bengal, gold nanoparticles, singlet oxygen generation, photodynamic therapyhttps://www.dovepress.com/light-triggered-liposomal-cargo-delivery-platform-incorporating-photos-peer-reviewed-article-IJNLiposomesRose BengalGold nanoparticlesSinglet oxygen generationPhotodynamic therapy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kautzka Z Clement S Goldys EM Deng W |
spellingShingle |
Kautzka Z Clement S Goldys EM Deng W Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity International Journal of Nanomedicine Liposomes Rose Bengal Gold nanoparticles Singlet oxygen generation Photodynamic therapy |
author_facet |
Kautzka Z Clement S Goldys EM Deng W |
author_sort |
Kautzka Z |
title |
Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity |
title_short |
Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity |
title_full |
Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity |
title_fullStr |
Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity |
title_full_unstemmed |
Light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity |
title_sort |
light-triggered liposomal cargo delivery platform incorporating photosensitizers and gold nanoparticles for enhanced singlet oxygen generation and increased cytotoxicity |
publisher |
Dove Medical Press |
series |
International Journal of Nanomedicine |
issn |
1178-2013 |
publishDate |
2017-02-01 |
description |
Zofia Kautzka, Sandhya Clement, Ewa M Goldys, Wei Deng Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, North Ryde, Sydney, NSW, Australia Abstract: We developed light-triggered liposomes incorporating 3–5 nm hydrophobic gold nanoparticles and Rose Bengal (RB), a well-known photosensitizer used for photodynamic therapy. Singlet oxygen generated by these liposomes with 532 nm light illumination was characterized for varying the molar ratio of lipids and gold nanoparticles while keeping the amount of RB constant. Gold nanoparticles were found to enhance the singlet oxygen generation rate, with a maximum enhancement factor of 1.75 obtained for the molar ratio of hydrogenated soy l-α-phosphatidylcholine:1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(hexanoylamine):gold of 57:5:17 compared with liposomes loaded with RB alone. The experimental results could be explained by the local electric field enhancement caused by gold nanoparticles. We further assessed cellular cytotoxicity of gold-loaded liposomes by encapsulating an antitumor drug, doxorubicin (Dox); such Dox-loaded liposomes were applied to human colorectal cancer cells (HCT116) and exposed to light. Gold-loaded liposomes containing RB and Dox where Dox release was triggered by light were found to exhibit higher cytotoxicity compared with the liposomes loaded with RB and Dox alone. Our results indicate that gold-loaded liposomes incorporating photosensitizers may serve as improved agents in photodynamic therapy and chemotherapy. Keywords: liposomes, Rose Bengal, gold nanoparticles, singlet oxygen generation, photodynamic therapy |
topic |
Liposomes Rose Bengal Gold nanoparticles Singlet oxygen generation Photodynamic therapy |
url |
https://www.dovepress.com/light-triggered-liposomal-cargo-delivery-platform-incorporating-photos-peer-reviewed-article-IJN |
work_keys_str_mv |
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