Gliding Associated Proteins Play Essential Roles during the Formation of the Inner Membrane Complex of Toxoplasma gondii.
The inner membrane complex (IMC) of apicomplexan parasites is a specialised structure localised beneath the parasite's plasma membrane, and is important for parasite stability and intracellular replication. Furthermore, it serves as an anchor for the myosin A motor complex, termed the glideosom...
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2016-02-01
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Series: | PLoS Pathogens |
Online Access: | https://doi.org/10.1371/journal.ppat.1005403 |
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doaj-dfa1e857c76443f4a33eab15eb97bdca2021-04-21T17:53:05ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742016-02-01122e100540310.1371/journal.ppat.1005403Gliding Associated Proteins Play Essential Roles during the Formation of the Inner Membrane Complex of Toxoplasma gondii.Clare R HardingSaskia EgarterMatthew GowElena Jiménez-RuizDavid J P FergusonMarkus MeissnerThe inner membrane complex (IMC) of apicomplexan parasites is a specialised structure localised beneath the parasite's plasma membrane, and is important for parasite stability and intracellular replication. Furthermore, it serves as an anchor for the myosin A motor complex, termed the glideosome. While the role of this protein complex in parasite motility and host cell invasion has been well described, additional roles during the asexual life cycle are unknown. Here, we demonstrate that core elements of the glideosome, the gliding associated proteins GAP40 and GAP50 as well as members of the GAPM family, have critical roles in the biogenesis of the IMC during intracellular replication. Deletion or disruption of these genes resulted in the rapid collapse of developing parasites after initiation of the cell cycle and led to redistribution of other glideosome components.https://doi.org/10.1371/journal.ppat.1005403 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Clare R Harding Saskia Egarter Matthew Gow Elena Jiménez-Ruiz David J P Ferguson Markus Meissner |
spellingShingle |
Clare R Harding Saskia Egarter Matthew Gow Elena Jiménez-Ruiz David J P Ferguson Markus Meissner Gliding Associated Proteins Play Essential Roles during the Formation of the Inner Membrane Complex of Toxoplasma gondii. PLoS Pathogens |
author_facet |
Clare R Harding Saskia Egarter Matthew Gow Elena Jiménez-Ruiz David J P Ferguson Markus Meissner |
author_sort |
Clare R Harding |
title |
Gliding Associated Proteins Play Essential Roles during the Formation of the Inner Membrane Complex of Toxoplasma gondii. |
title_short |
Gliding Associated Proteins Play Essential Roles during the Formation of the Inner Membrane Complex of Toxoplasma gondii. |
title_full |
Gliding Associated Proteins Play Essential Roles during the Formation of the Inner Membrane Complex of Toxoplasma gondii. |
title_fullStr |
Gliding Associated Proteins Play Essential Roles during the Formation of the Inner Membrane Complex of Toxoplasma gondii. |
title_full_unstemmed |
Gliding Associated Proteins Play Essential Roles during the Formation of the Inner Membrane Complex of Toxoplasma gondii. |
title_sort |
gliding associated proteins play essential roles during the formation of the inner membrane complex of toxoplasma gondii. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2016-02-01 |
description |
The inner membrane complex (IMC) of apicomplexan parasites is a specialised structure localised beneath the parasite's plasma membrane, and is important for parasite stability and intracellular replication. Furthermore, it serves as an anchor for the myosin A motor complex, termed the glideosome. While the role of this protein complex in parasite motility and host cell invasion has been well described, additional roles during the asexual life cycle are unknown. Here, we demonstrate that core elements of the glideosome, the gliding associated proteins GAP40 and GAP50 as well as members of the GAPM family, have critical roles in the biogenesis of the IMC during intracellular replication. Deletion or disruption of these genes resulted in the rapid collapse of developing parasites after initiation of the cell cycle and led to redistribution of other glideosome components. |
url |
https://doi.org/10.1371/journal.ppat.1005403 |
work_keys_str_mv |
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