Highly Selective Oxidation of 5-Hydroxymethylfurfural to 5-Hydroxymethyl-2-Furancarboxylic Acid by a Robust Whole-Cell Biocatalyst
Value-added utilization of biomass-derived 5-hydroxymethylfurfural (HMF) to produce useful derivatives is of great interest. In this work, extremely radiation resistant <i>Deinococcus wulumuqiensis</i> R12 was explored for the first time as a new robust biocatalyst for selective oxidatio...
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doaj-dfa38801489441439e29d6ca28cc58d82020-11-25T02:23:39ZengMDPI AGCatalysts2073-43442019-06-019652610.3390/catal9060526catal9060526Highly Selective Oxidation of 5-Hydroxymethylfurfural to 5-Hydroxymethyl-2-Furancarboxylic Acid by a Robust Whole-Cell BiocatalystRan Cang0Li-Qun Shen1Guang Yang2Zhi-Dong Zhang3He Huang4Zhi-Gang Zhang5School of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhu Road(S), Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhu Road(S), Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhu Road(S), Nanjing 211816, ChinaInstitute of Microbiology, Xinjiang Academy of Agricultural Sciences, 403 Nanchang Rd, Wulumuqi 830091, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhu Road(S), Nanjing 211816, ChinaSchool of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhu Road(S), Nanjing 211816, ChinaValue-added utilization of biomass-derived 5-hydroxymethylfurfural (HMF) to produce useful derivatives is of great interest. In this work, extremely radiation resistant <i>Deinococcus wulumuqiensis</i> R12 was explored for the first time as a new robust biocatalyst for selective oxidation of HMF to 5-hydroxymethylfuroic acid (HMFCA). Its resting cells exhibited excellent catalytic performance in a broad range of pH and temperature values, and extremely high tolerance to HMF and the HMFCA product. An excellent yield of HMFCA (up to 90%) was achieved when the substrate concentration was set to 300 mM under the optimized reaction conditions. In addition, 511 mM of product was obtained within 20 h by employing a fed-batch strategy, affording a productivity of 44 g/L per day. Of significant synthetic interest was the finding that the <i>D. wulumuqiensis</i> R12 cells were able to catalyze the selective oxidation of other structurally diverse aldehydes to their corresponding acids with good yield and high selectivity, indicating broad substrate scope and potential widespread applications in biotechnology and organic chemistry.https://www.mdpi.com/2073-4344/9/6/526biocatalysisextremophile5-hydroxymethylfurfural5-hydroxymethylfuroic acidplatform chemicalswhole cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ran Cang Li-Qun Shen Guang Yang Zhi-Dong Zhang He Huang Zhi-Gang Zhang |
spellingShingle |
Ran Cang Li-Qun Shen Guang Yang Zhi-Dong Zhang He Huang Zhi-Gang Zhang Highly Selective Oxidation of 5-Hydroxymethylfurfural to 5-Hydroxymethyl-2-Furancarboxylic Acid by a Robust Whole-Cell Biocatalyst Catalysts biocatalysis extremophile 5-hydroxymethylfurfural 5-hydroxymethylfuroic acid platform chemicals whole cells |
author_facet |
Ran Cang Li-Qun Shen Guang Yang Zhi-Dong Zhang He Huang Zhi-Gang Zhang |
author_sort |
Ran Cang |
title |
Highly Selective Oxidation of 5-Hydroxymethylfurfural to 5-Hydroxymethyl-2-Furancarboxylic Acid by a Robust Whole-Cell Biocatalyst |
title_short |
Highly Selective Oxidation of 5-Hydroxymethylfurfural to 5-Hydroxymethyl-2-Furancarboxylic Acid by a Robust Whole-Cell Biocatalyst |
title_full |
Highly Selective Oxidation of 5-Hydroxymethylfurfural to 5-Hydroxymethyl-2-Furancarboxylic Acid by a Robust Whole-Cell Biocatalyst |
title_fullStr |
Highly Selective Oxidation of 5-Hydroxymethylfurfural to 5-Hydroxymethyl-2-Furancarboxylic Acid by a Robust Whole-Cell Biocatalyst |
title_full_unstemmed |
Highly Selective Oxidation of 5-Hydroxymethylfurfural to 5-Hydroxymethyl-2-Furancarboxylic Acid by a Robust Whole-Cell Biocatalyst |
title_sort |
highly selective oxidation of 5-hydroxymethylfurfural to 5-hydroxymethyl-2-furancarboxylic acid by a robust whole-cell biocatalyst |
publisher |
MDPI AG |
series |
Catalysts |
issn |
2073-4344 |
publishDate |
2019-06-01 |
description |
Value-added utilization of biomass-derived 5-hydroxymethylfurfural (HMF) to produce useful derivatives is of great interest. In this work, extremely radiation resistant <i>Deinococcus wulumuqiensis</i> R12 was explored for the first time as a new robust biocatalyst for selective oxidation of HMF to 5-hydroxymethylfuroic acid (HMFCA). Its resting cells exhibited excellent catalytic performance in a broad range of pH and temperature values, and extremely high tolerance to HMF and the HMFCA product. An excellent yield of HMFCA (up to 90%) was achieved when the substrate concentration was set to 300 mM under the optimized reaction conditions. In addition, 511 mM of product was obtained within 20 h by employing a fed-batch strategy, affording a productivity of 44 g/L per day. Of significant synthetic interest was the finding that the <i>D. wulumuqiensis</i> R12 cells were able to catalyze the selective oxidation of other structurally diverse aldehydes to their corresponding acids with good yield and high selectivity, indicating broad substrate scope and potential widespread applications in biotechnology and organic chemistry. |
topic |
biocatalysis extremophile 5-hydroxymethylfurfural 5-hydroxymethylfuroic acid platform chemicals whole cells |
url |
https://www.mdpi.com/2073-4344/9/6/526 |
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