Calcium imaging of adult-born neurons in freely moving mice

Calcium imaging of adult-born neurons in freely moving mice

Summary: Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca2+ imaging of ABN activity using a miniaturized fluores...

Full description

Bibliographic Details
Main Authors: Alvaro Carrier-Ruiz, Yuki Sugaya, Deependra Kumar, Pablo Vergara, Iyo Koyanagi, Sakthivel Srinivasan, Toshie Naoi, Masanobu Kano, Masanori Sakaguchi
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Microscopy
Model Organisms
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302252
id doaj-dfbb9671ba0b4bb0bbadaaac38163366
record_format Article
spelling doaj-dfbb9671ba0b4bb0bbadaaac381633662021-03-22T12:52:45ZengElsevierSTAR Protocols2666-16672021-03-0121100238Calcium imaging of adult-born neurons in freely moving miceAlvaro Carrier-Ruiz0Yuki Sugaya1Deependra Kumar2Pablo Vergara3Iyo Koyanagi4Sakthivel Srinivasan5Toshie Naoi6Masanobu Kano7Masanori Sakaguchi8Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan; International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo Institutes for Advanced Study (UTIAS), Tokyo 113-0033, Japan; Department of Neuroscience, Karolinska Institute, Stockholm 17165, SwedenDepartment of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan; International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo Institutes for Advanced Study (UTIAS), Tokyo 113-0033, Japan; Corresponding authorInternational Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki 305-0006, JapanInternational Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki 305-0006, JapanInternational Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki 305-0006, JapanInternational Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki 305-0006, JapanInternational Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki 305-0006, JapanDepartment of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan; International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo Institutes for Advanced Study (UTIAS), Tokyo 113-0033, JapanInternational Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki 305-0006, Japan; Corresponding authorSummary: Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca2+ imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings. As preparatory surgery and post-recording data processing can be major obstacles, we provide detailed descriptions and problem-solving tips.For complete details on the use and execution of this protocol, please refer to Kumar et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720302252MicroscopyModel OrganismsNeuroscience
collection DOAJ
language English
format Article
sources DOAJ
author Alvaro Carrier-Ruiz
Yuki Sugaya
Deependra Kumar
Pablo Vergara
Iyo Koyanagi
Sakthivel Srinivasan
Toshie Naoi
Masanobu Kano
Masanori Sakaguchi
spellingShingle Alvaro Carrier-Ruiz
Yuki Sugaya
Deependra Kumar
Pablo Vergara
Iyo Koyanagi
Sakthivel Srinivasan
Toshie Naoi
Masanobu Kano
Masanori Sakaguchi
Calcium imaging of adult-born neurons in freely moving mice
STAR Protocols
Microscopy
Model Organisms
Neuroscience
author_facet Alvaro Carrier-Ruiz
Yuki Sugaya
Deependra Kumar
Pablo Vergara
Iyo Koyanagi
Sakthivel Srinivasan
Toshie Naoi
Masanobu Kano
Masanori Sakaguchi
author_sort Alvaro Carrier-Ruiz
title Calcium imaging of adult-born neurons in freely moving mice
title_short Calcium imaging of adult-born neurons in freely moving mice
title_full Calcium imaging of adult-born neurons in freely moving mice
title_fullStr Calcium imaging of adult-born neurons in freely moving mice
title_full_unstemmed Calcium imaging of adult-born neurons in freely moving mice
title_sort calcium imaging of adult-born neurons in freely moving mice
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-03-01
description Summary: Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca2+ imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings. As preparatory surgery and post-recording data processing can be major obstacles, we provide detailed descriptions and problem-solving tips.For complete details on the use and execution of this protocol, please refer to Kumar et al. (2020).
topic Microscopy
Model Organisms
Neuroscience
url http://www.sciencedirect.com/science/article/pii/S2666166720302252
work_keys_str_mv AT alvarocarrierruiz calciumimagingofadultbornneuronsinfreelymovingmice
AT yukisugaya calciumimagingofadultbornneuronsinfreelymovingmice
AT deependrakumar calciumimagingofadultbornneuronsinfreelymovingmice
AT pablovergara calciumimagingofadultbornneuronsinfreelymovingmice
AT iyokoyanagi calciumimagingofadultbornneuronsinfreelymovingmice
AT sakthivelsrinivasan calciumimagingofadultbornneuronsinfreelymovingmice
AT toshienaoi calciumimagingofadultbornneuronsinfreelymovingmice
AT masanobukano calciumimagingofadultbornneuronsinfreelymovingmice
AT masanorisakaguchi calciumimagingofadultbornneuronsinfreelymovingmice
_version_ 1724207618183921664

Similar Items